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release (latest)](https://img.shields.io/github/v/release/ablab/spades)](https://github.com/ablab/spades/releases/)\n[![GitHub Downloads](https://img.shields.io/github/downloads/ablab/spades/total.svg?style=social\u0026logo=github\u0026label=Download)](https://github.com/ablab/spades/releases)\n[![BioConda Downloads](https://anaconda.org/bioconda/spades/badges/downloads.svg)](https://anaconda.org/bioconda/spades)\n[![Contributor Covenant](https://img.shields.io/badge/Contributor%20Covenant-2.0-4baaaa.svg)](code_of_conduct.md)\n\n\n# About SPAdes\n\nSPAdes is a versatile toolkit designed for assembly and analysis of sequencing data.\nSPAdes is primarily developed for Illumina sequencing data, but can be used for IonTorrent as well. Most of SPAdes pipelines support hybrid mode, i.e. allow using long reads (PacBio and Oxford Nanopore) as a supplementary data.\n\nSPAdes package contains assembly pipelines for isolated and single-cell bacterial, as well as metagenomic and transcriptomic data.\nAdditional modes allow to discover bacterial plasmids and RNA viruses, as well as perform HMM-guided assembly.\nBesides, SPAdes package includes supplementary tools for efficient k-mer counting and k-mer-based read filtering, assembly graph construction and simplification, sequence-to-graph alignment and metagenomic binning refinement.\n\n- [Complete SPAdes user manual](https://ablab.github.io/spades/)\n\n- [SPAdes download page](https://github.com/ablab/spades/releases/latest/)\n\n- [Latest SPAdes publication](https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpbi.102)\n\nCurrent version: see `VERSION` file.\n\n# Quick start\n\n- Complete user manual can be found [here](https://ablab.github.io/spades/). Information below is provided merely for your convenience and cannot be considered as the user guide.\n\n- SPAdes assembler supports:\n  - Assembly of second-generation sequencing data (Illumina or IonTorrent);\n  - PacBio and Nanopore reads that are used as supplementary data only.\n\n- SPAdes allows to assemble genomes, metagenomes, transcriptomes, viral genomes etc.\n\n- Download SPAdes binaries for Linux or MacOS [here](https://github.com/ablab/spades/releases/latest/). You can also compile SPAdes from [source](https://github.com/ablab/spades/releases/latest/) (requires g++ 9.0+, cmake 3.16+, zlib and libbz2). SPAdes requires only Python 3.8+ to be installed.\n\n- Test your SPAdes installation by running\n\n```\n    bin/spades.py --test\n```\n\n- A single paired-end library (separate files, gzipped):\n\n```\n    bin/spades.py -1 left.fastq.gz -2 right.fastq.gz -o output_folder\n```\n\n\n- IonTorrent data:\n```\n    bin/spades.py --iontorrent -s it_reads.fastq -o output_folder\n```\n\n- A paired-end library coupled with long PacBio reads:\n\n```\n    bin/spades.py -1 left.fastq.gz -2 right.fastq.gz --pacbio pb.fastq -o output_folder\n```\n\n- Available assembly modes: `--isolate`, `--sc`, `--plasmid`, `--meta`, `--metaplasmid`, `--metaviral`, `--rna`, `--rnaviral`,  `--bio`, `--corona`, `--sewage`.\n\n- Standalone tools in SPAdes package: k-mer counting, k-mer cardinality estimation, k-mer-based read filtering, assembly graph construction, assembly graph simplification, alignment of long reads to an assembly graph, refinement of metagenomic binning.\n\n\n# Citation\nIf you use SPAdes in your research, please cite [our latest paper](https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpbi.102).\n\nIn case you perform hybrid assembly using  PacBio or Nanopore reads, you may also cite [Antipov et al., 2015](http://bioinformatics.oxfordjournals.org/content/early/2015/11/20/bioinformatics.btv688.short). If you use multiple paired-end and/or mate-pair libraries you may additionally cite papers describing SPAdes repeat resolution algorithms [Prjibelski et al., 2014](http://bioinformatics.oxfordjournals.org/content/30/12/i293.short) and [Vasilinetc et al., 2015](http://bioinformatics.oxfordjournals.org/content/31/20/3262.abstract). \n\nIf you use other pipelines, please cite the following papers:\n\n-   metaSPAdes: [Nurk et al., 2017](https://genome.cshlp.org/content/27/5/824.short).\n-   plasmidSPAdes: [Antipov et al., 2016](https://academic.oup.com/bioinformatics/article/32/22/3380/2525610).\n-   metaplasmidSPAdes / plasmidVerify: [Antipov et al., 2019](https://genome.cshlp.org/content/29/6/961.short)\n-   metaviralSPAdes / viralVerify: [Antipov et al., 2020](https://academic.oup.com/bioinformatics/article-abstract/36/14/4126/5837667)\n-   rnaSPAdes: [Bushmanova et al., 2019](https://academic.oup.com/gigascience/article/8/9/giz100/5559527).\n-   biosyntheticSPAdes: [Meleshko et al., 2019](https://genome.cshlp.org/content/early/2019/06/03/gr.243477.118?top=1).\n-   coronaSPAdes: [Meleshko et al., 2022](https://academic.oup.com/bioinformatics/article/38/1/1/6354349).\n\nYou may also include older papers [Nurk, Bankevich et al., 2013](http://link.springer.com/chapter/10.1007%2F978-3-642-37195-0_13) or [Bankevich, Nurk et al., 2012](http://online.liebertpub.com/doi/abs/10.1089/cmb.2012.0021), especially if you assemble single-cell data.\n\n\n# Feedback and bug reports\n\nPlease, leave your comments and bug reports at [our GitHub repository tracker](https://github.com/ablab/spades/issues). If you have any troubles running SPAdes, please attach `params.txt` and `spades.log` from the output folder.\n\n","funding_links":[],"categories":["Next Generation Sequencing","Popular Packages"],"sub_categories":["Assembly"],"project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fablab%2Fspades","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Fablab%2Fspades","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fablab%2Fspades/lists"}