{"id":29038698,"url":"https://github.com/adelia567/crispr","last_synced_at":"2025-06-26T14:02:17.793Z","repository":{"id":298681332,"uuid":"1000629096","full_name":"Adelia567/Crispr","owner":"Adelia567","description":"Crispr is a web app that simplifies the design and analysis of CRISPR guide RNAs, making it accessible for everyone. 🧬 With features like off-target scanning and AI reporting, users can quickly generate and evaluate their gRNA options. 🐙","archived":false,"fork":false,"pushed_at":"2025-06-26T10:36:12.000Z","size":162,"stargazers_count":0,"open_issues_count":0,"forks_count":0,"subscribers_count":0,"default_branch":"main","last_synced_at":"2025-06-26T11:42:04.131Z","etag":null,"topics":["amplicon-sequences","awesome","bioconductor-package","bioinformatics","cas","crispr-analysis","crispr-cas-systems","crispr-subtypes","grna","grna-sequence","nf-core","ngs","pipeline","python","quantification","sanger","sgrna-design","workflow"],"latest_commit_sha":null,"homepage":null,"language":"Python","has_issues":false,"has_wiki":null,"has_pages":null,"mirror_url":null,"source_name":null,"license":"apache-2.0","status":null,"scm":"git","pull_requests_enabled":true,"icon_url":"https://github.com/Adelia567.png","metadata":{"files":{"readme":"README.md","changelog":null,"contributing":"CONTRIBUTING.md","funding":null,"license":"LICENSE","code_of_conduct":null,"threat_model":null,"audit":null,"citation":null,"codeowners":null,"security":null,"support":null,"governance":null,"roadmap":null,"authors":null,"dei":null,"publiccode":null,"codemeta":null,"zenodo":null}},"created_at":"2025-06-12T04:51:29.000Z","updated_at":"2025-06-26T10:36:15.000Z","dependencies_parsed_at":"2025-06-26T11:40:33.491Z","dependency_job_id":null,"html_url":"https://github.com/Adelia567/Crispr","commit_stats":null,"previous_names":["adelia567/crispr"],"tags_count":1,"template":false,"template_full_name":null,"purl":"pkg:github/Adelia567/Crispr","repository_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Adelia567%2FCrispr","tags_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Adelia567%2FCrispr/tags","releases_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Adelia567%2FCrispr/releases","manifests_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Adelia567%2FCrispr/manifests","owner_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners/Adelia567","download_url":"https://codeload.github.com/Adelia567/Crispr/tar.gz/refs/heads/main","sbom_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Adelia567%2FCrispr/sbom","host":{"name":"GitHub","url":"https://github.com","kind":"github","repositories_count":262081072,"owners_count":23255657,"icon_url":"https://github.com/github.png","version":null,"created_at":"2022-05-30T11:31:42.601Z","updated_at":"2022-07-04T15:15:14.044Z","host_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub","repositories_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories","repository_names_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repository_names","owners_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners"}},"keywords":["amplicon-sequences","awesome","bioconductor-package","bioinformatics","cas","crispr-analysis","crispr-cas-systems","crispr-subtypes","grna","grna-sequence","nf-core","ngs","pipeline","python","quantification","sanger","sgrna-design","workflow"],"created_at":"2025-06-26T14:00:59.016Z","updated_at":"2025-06-26T14:02:17.769Z","avatar_url":"https://github.com/Adelia567.png","language":"Python","funding_links":[],"categories":[],"sub_categories":[],"readme":"# Crispr: AI-Powered CRISPR gRNA Design and Analysis 🌱🧬\n\n![Crispr Logo](https://via.placeholder.com/150)\n\nWelcome to the **Crispr** repository! This project aims to accelerate DNA gene editing projects through intuitive, AI-powered design and analysis of CRISPR gRNA. Whether you're a researcher, student, or hobbyist, this tool simplifies the complexities of gene editing, allowing you to focus on what matters most—your research.\n\n## Table of Contents\n\n1. [Introduction](#introduction)\n2. [Features](#features)\n3. [Installation](#installation)\n4. [Usage](#usage)\n5. [Technologies Used](#technologies-used)\n6. [Contributing](#contributing)\n7. [License](#license)\n8. [Releases](#releases)\n9. [Contact](#contact)\n\n## Introduction\n\nCRISPR technology has revolutionized genetic engineering. With our tool, you can design guide RNAs (gRNAs) quickly and efficiently. The AI-powered features analyze DNA sequences and suggest optimal gRNA candidates, making the process more straightforward than ever. \n\nTo get started, visit our [Releases](https://github.com/Adelia567/Crispr/releases) section to download the latest version of the tool.\n\n## Features\n\n- **AI-Powered gRNA Design**: Our intelligent algorithms suggest the best gRNA sequences based on your input DNA.\n- **User-Friendly Interface**: Built with Streamlit, the app offers an intuitive web interface for easy navigation.\n- **Comprehensive Analysis**: Evaluate the effectiveness of your gRNA with built-in analytical tools.\n- **Customizable Options**: Tailor the gRNA design parameters to fit your specific project needs.\n- **Guide RNA Library**: Access a curated library of pre-designed gRNAs for common genes.\n\n## Installation\n\nTo set up the Crispr tool, follow these steps:\n\n1. **Clone the Repository**:\n   ```bash\n   git clone https://github.com/Adelia567/Crispr.git\n   ```\n\n2. **Navigate to the Directory**:\n   ```bash\n   cd Crispr\n   ```\n\n3. **Install Dependencies**:\n   Use pip to install the required packages:\n   ```bash\n   pip install -r requirements.txt\n   ```\n\n4. **Run the Application**:\n   Start the Streamlit app with:\n   ```bash\n   streamlit run app.py\n   ```\n\n## Usage\n\nOnce the application is running, follow these steps to design your gRNA:\n\n1. **Input DNA Sequence**: Paste your target DNA sequence into the designated field.\n2. **Select Parameters**: Adjust any optional parameters for gRNA design.\n3. **Analyze Results**: Click the 'Design gRNA' button to generate suggestions.\n4. **Review Suggestions**: The tool will display the recommended gRNAs along with their scores and potential off-target effects.\n\nFor detailed instructions and examples, check the user guide included in the repository.\n\n## Technologies Used\n\n- **Python**: The primary programming language for the application.\n- **Streamlit**: A powerful library for building web applications.\n- **Biopython**: For handling biological data.\n- **NumPy and Pandas**: For data manipulation and analysis.\n- **SciPy**: For scientific computations.\n\n## Contributing\n\nWe welcome contributions from the community! If you want to help improve Crispr, follow these steps:\n\n1. **Fork the Repository**: Create your own copy of the project.\n2. **Create a New Branch**: Work on your feature or bug fix in a separate branch.\n   ```bash\n   git checkout -b feature-name\n   ```\n3. **Commit Your Changes**: Make sure to write clear commit messages.\n   ```bash\n   git commit -m \"Add new feature\"\n   ```\n4. **Push to Your Fork**: Send your changes to your GitHub repository.\n   ```bash\n   git push origin feature-name\n   ```\n5. **Create a Pull Request**: Submit your changes for review.\n\n## License\n\nThis project is licensed under the MIT License. See the [LICENSE](LICENSE) file for details.\n\n## Releases\n\nFor the latest updates and versions, visit our [Releases](https://github.com/Adelia567/Crispr/releases) section. Download the latest release and follow the installation instructions to get started.\n\n## Contact\n\nFor questions, suggestions, or issues, please open an issue in the repository or contact the maintainers:\n\n- **Adelia**: [Adelia567](https://github.com/Adelia567)\n\n---\n\nThank you for your interest in Crispr! We hope this tool helps you in your gene editing projects. Your feedback is invaluable, and we look forward to your contributions!","project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fadelia567%2Fcrispr","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Fadelia567%2Fcrispr","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fadelia567%2Fcrispr/lists"}