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`moclo` [![Stars](https://img.shields.io/github/stars/althonos/moclo.svg?style=social\u0026maxAge=3600\u0026label=Star)](https://github.com/althonos/moclo/stargazers)\n\n*A Python implementation of the [MoClo](https://www.addgene.org/cloning/moclo/) system logic.*\n\n[![Source](https://img.shields.io/badge/source-GitHub-303030.svg?maxAge=3600\u0026style=flat-square)](https://github.com/althonos/moclo)\n[![Build](https://img.shields.io/github/workflow/status/althonos/moclo/Test?style=flat-square\u0026maxAge=3600)](https://github.com/althonos/moclo/actions)\n[![Docs](https://img.shields.io/readthedocs/moclo.svg?maxAge=3600\u0026style=flat-square)](https://moclo.readthedocs.io/)\n[![Codecov](https://img.shields.io/codecov/c/github/althonos/moclo/master.svg?style=flat-square\u0026maxAge=600)](https://codecov.io/gh/althonos/moclo)\n[![Codacy](https://img.shields.io/codacy/grade/5b29a9c0d91f4e82944a46997bd9a480/master.svg?style=flat-square\u0026maxAge=300)](https://www.codacy.com/app/althonos/moclo)\n[![License](https://img.shields.io/pypi/l/moclo.svg?style=flat-square\u0026maxAge=300)](https://choosealicense.com/licenses/mit/)\n[![DOI](https://img.shields.io/badge/doi-10.5281%2Fzenodo.1401815-blue.svg?style=flat-square\u0026maxAge=31536000)](https://zenodo.org/badge/latestdoi/138012703)\n\n## 📚 Documentation\n\nThe documentation is hosted on [ReadTheDocs](https://moclo.readthedocs.org),\nand built against the latest commit of the development repository. It contains\na comprehensive API reference as well as examples compiled from Jupyter\nnotebooks at each build.\n\n\n## 🔩 Base module\n\nThe base logic is handled by the core [`moclo`](https://github.com/althonos/moclo/tree/master/moclo)\nmodule. It embeds an object model of the MoClo system logic, but does not enforce\nany specific sequence structure, and is not usable alone. You must install a kit\n(listed below) to be able to validate and compute assemblies.\n\n\n## 🧰 Kits\n\nAdditional kits can be installed separately depending on what's needed. The\nfollowing implementations are available:\n\n* [Original MoClo (`moclo-moclo`)](https://github.com/althonos/moclo/tree/master/moclo-moclo)\n* [Yeast ToolKit and Pichia ToolKit (`moclo-ytk`)](https://github.com/althonos/moclo/tree/master/moclo-ytk)\n* [CIDAR Kit (`moclo-cidar`)](https://github.com/althonos/moclo/tree/master/moclo-cidar)\n* [EcoFlex Kit (`moclo-ecoflex`)](https://github.com/althonos/moclo/tree/master/moclo-ecoflex)\n\nOnce installed, kits are available in the `moclo.kits` namespace module.\n[Kit-specific documentation](https://moclo.readthedocs.io/en/latest/#kits) is\navailable as well.\n\n\n## 🗂️ Registries\n\nKit-specific modules and vectors are distributed with the library files, so that\neach library provides the base parts needed to create an assembly. They can be\nfound in the `moclo.registry` namespace. See also the documentation of each\n`moclo.registry` submodule for a detail of how sequences were obtained. The\nembedded sequences are distributed in GenBank format with the source distributions\nof each plugin.\n\n\n## 🗒️ Notebook\n\n[![Docker Build Status](https://img.shields.io/docker/build/althonos/moclo.svg?style=flat-square\u0026maxAge=3600)](https://hub.docker.com/r/althonos/moclo/builds/) [![Docker Pulls](https://img.shields.io/docker/pulls/althonos/moclo.svg?style=flat-square\u0026maxAge=3600)](https://hub.docker.com/r/althonos/moclo/)\n\nThis repository provides a YTK-specific Jupyter notebook as a Docker image,\nwhich can be used to generate a protocol for YTK MoClo assembly. Run it locally\nusing the following command:\n```console\ndocker run --rm -it -p 8888:8888 althonos/moclo\n```\nand visit [https://localhost:8888/](https://localhost:8888/) to start interacting\nwith the notebook.\n\n\n## ⚖️ License\n\nThis project is licensed under the [MIT License](http://choosealicense.com/licenses/mit/).\n\n*This project is in no way affiliated, sponsored, or otherwise endorsed by [Addgene](https://www.addgene.org) or any of the MoClo toolkit creators.\nIt was developed by [Martin Larralde](https://github.com/althonos/pyhmmer)\nduring a placement at the [InBio team](https://research.pasteur.fr/en/team/experimental-and-computational-methods-for-modeling-cellular-processes/)\nat the Institut Pasteur of Paris during the summer of 2018.*\n","project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Falthonos%2Fmoclo","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Falthonos%2Fmoclo","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Falthonos%2Fmoclo/lists"}