{"id":20837751,"url":"https://github.com/astrazeneca/fragler","last_synced_at":"2026-01-24T16:06:40.675Z","repository":{"id":44333615,"uuid":"510600299","full_name":"AstraZeneca/fragler","owner":"AstraZeneca","description":null,"archived":false,"fork":false,"pushed_at":"2022-07-06T12:11:23.000Z","size":20,"stargazers_count":4,"open_issues_count":0,"forks_count":0,"subscribers_count":2,"default_branch":"main","last_synced_at":"2025-09-09T11:50:58.340Z","etag":null,"topics":[],"latest_commit_sha":null,"homepage":null,"language":"Python","has_issues":true,"has_wiki":null,"has_pages":null,"mirror_url":null,"source_name":null,"license":"apache-2.0","status":null,"scm":"git","pull_requests_enabled":true,"icon_url":"https://github.com/AstraZeneca.png","metadata":{"files":{"readme":"README.md","changelog":null,"contributing":"CONTRIBUTING.md","funding":null,"license":"LICENSE.md","code_of_conduct":null,"threat_model":null,"audit":null,"citation":null,"codeowners":null,"security":null,"support":null}},"created_at":"2022-07-05T05:36:03.000Z","updated_at":"2025-04-30T13:44:51.000Z","dependencies_parsed_at":"2022-09-10T01:41:32.007Z","dependency_job_id":null,"html_url":"https://github.com/AstraZeneca/fragler","commit_stats":null,"previous_names":[],"tags_count":0,"template":false,"template_full_name":null,"purl":"pkg:github/AstraZeneca/fragler","repository_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/AstraZeneca%2Ffragler","tags_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/AstraZeneca%2Ffragler/tags","releases_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/AstraZeneca%2Ffragler/releases","manifests_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/AstraZeneca%2Ffragler/manifests","owner_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners/AstraZeneca","download_url":"https://codeload.github.com/AstraZeneca/fragler/tar.gz/refs/heads/main","sbom_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/AstraZeneca%2Ffragler/sbom","scorecard":null,"host":{"name":"GitHub","url":"https://github.com","kind":"github","repositories_count":286080680,"owners_count":28730916,"icon_url":"https://github.com/github.png","version":null,"created_at":"2022-05-30T11:31:42.601Z","updated_at":"2026-01-24T10:24:43.181Z","status":"ssl_error","status_checked_at":"2026-01-24T10:24:36.112Z","response_time":89,"last_error":"SSL_read: unexpected eof while reading","robots_txt_status":"success","robots_txt_updated_at":"2025-07-24T06:49:26.215Z","robots_txt_url":"https://github.com/robots.txt","online":false,"can_crawl_api":true,"host_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub","repositories_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories","repository_names_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repository_names","owners_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners"}},"keywords":[],"created_at":"2024-11-18T01:08:29.875Z","updated_at":"2026-01-24T16:06:40.656Z","avatar_url":"https://github.com/AstraZeneca.png","language":"Python","funding_links":[],"categories":[],"sub_categories":[],"readme":"![Maturity level-0](https://img.shields.io/badge/Maturity%20Level-ML--0-red)\n\n# Fragler\n\n## Overview\n\nThis tool implements an alogrithm to recycle fragments for modular DNA assembley. For details, see Öling et al (\"FRAGLER: A Fragment Recycler Application Enabling Rapid and Scalable Modular DNA Assembly\").\n\n## Dependencies\n\nThe tool uses [conda](https://docs.conda.io/en/latest/) to manage its dependencies. Follow installation instructions in either the official conda repository or any other conda flavour. We recommend [mamba](https://github.com/mamba-org/mamba) as a drop-in replacement for conda.\n\nThe tool is also dependent on acces to the GeneArt API (https://www.thermofisher.com/se/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis.html) and requires an access key. To obtain an access key, see instructions http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/geneart-gene-synthesis-api-presentation.pdf  and contact geneartapi@thermofisher.com \n\n## Installation instructions\n\n1. Clone the repo:\n```bash\ngit@github.com:DS-QuBi/golden-gate-cloning.git\n```\n2. Install dependencies\n```bash\ncd golden-gate-cloning\nconda env create -f environment.yaml -n golden-gate-cloning\n```\n\t\n## Usage\n1. Activate conda environment\n\n```bash\nconda activate golden-gate-cloning\n```\n\n2. Start the server\n```bash\nbash start_server.sh\n```\n3. Access the app through a browser using the url reported in the previous step\n","project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fastrazeneca%2Ffragler","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Fastrazeneca%2Ffragler","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fastrazeneca%2Ffragler/lists"}