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[Installation](https://github.com/crisprVerse/Tutorials/tree/master/Installation)\n2. [Building a genome index for off-target alignment](https://github.com/crisprVerse/Tutorials/tree/master/Building_Genome_Indices)\n3. [Specifying a custom nuclease](https://github.com/crisprVerse/Tutorials/tree/master/Building_Custom_Nuclease)\n4. [Building a gene annotation object](https://github.com/crisprVerse/Tutorials/tree/master/Building_Gene_Annotation)\n\n### Common gRNA design workflows\n\n5. [CRISPR knockout (CRISPRko) design with Cas9](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRko_Cas9)\n6. [CRISPR knockout (CRISPRko) design with Cas12a](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRko_Cas12a)\n7. [CRISPR knockdown (CRISPRkd) design with Cas13d](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRkd_CasRx)\n8. [CRISPR knockdown (CRISPRkd) design with the Csm complex](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRkd_Csm)\n9. [CRISPR base editing (CRISPRbe) design with BE4max](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRbe)\n10. [CRISPR activation (CRISPRa) design](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRa)\n11. [CRISPR interference (CRISPRi) design](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRi)\n\n\n### Less common gRNA design workflows\n\n12. [Paired gRNA design](https://github.com/crisprVerse/Tutorials/tree/master/Design_PairedGuides)\n13. [Working with custom DNA sequences](https://github.com/crisprVerse/Tutorials/tree/master/Design_Custom_Sequence)\n14. [Design for optical pooled screening (OPS)](https://github.com/crisprVerse/Tutorials/tree/master/Design_OPS)\n\n### Miscellaneous\n\n15. [Mapping gRNAs across species](https://github.com/crisprVerse/Tutorials/tree/master/Design_Cross_Reactivity)\n16. [Working with minor and major alleles](https://github.com/crisprVerse/Tutorials/tree/master/Design_Minor_Major_Allele)\n17. [Validating existing gRNA libraries](https://github.com/crisprVerse/Tutorials/tree/master/Validating_Existing_gRNA_Libraries)\n18. [Building a gRNA database across all protein-coding genes](https://github.com/crisprVerse/Tutorials/tree/master/Building_Database_Human_Cas9)\n\n","project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fcrisprverse%2Ftutorials","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Fcrisprverse%2Ftutorials","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fcrisprverse%2Ftutorials/lists"}