{"id":39464170,"url":"https://github.com/gear-genomics/indigo","last_synced_at":"2026-01-18T04:48:35.765Z","repository":{"id":18371168,"uuid":"84113506","full_name":"gear-genomics/indigo","owner":"gear-genomics","description":"Indigo: SNV and InDel Discovery in Chromatogram traces obtained from Sanger sequencing of PCR products","archived":false,"fork":false,"pushed_at":"2025-11-18T14:10:18.000Z","size":1253,"stargazers_count":36,"open_issues_count":0,"forks_count":9,"subscribers_count":3,"default_branch":"main","last_synced_at":"2025-11-18T16:36:37.819Z","etag":null,"topics":["alignment","alleles","chromatogram-traces","crispr-analysis","crispr-cas9","gear-genomics","indel-discovery","indels","indigo","pcr","pcr-products","sanger-chromatograms","sanger-sequencing","sequencing","variant-calling","variants"],"latest_commit_sha":null,"homepage":"https://www.gear-genomics.com/indigo/","language":"JavaScript","has_issues":true,"has_wiki":null,"has_pages":null,"mirror_url":null,"source_name":null,"license":"gpl-3.0","status":null,"scm":"git","pull_requests_enabled":true,"icon_url":"https://github.com/gear-genomics.png","metadata":{"files":{"readme":"README.md","changelog":null,"contributing":null,"funding":null,"license":"LICENSE","code_of_conduct":null,"threat_model":null,"audit":null,"citation":null,"codeowners":null,"security":null,"support":null,"governance":null,"roadmap":null,"authors":null,"dei":null,"publiccode":null,"codemeta":null,"zenodo":null,"notice":null,"maintainers":null,"copyright":null,"agents":null,"dco":null,"cla":null}},"created_at":"2017-03-06T19:42:06.000Z","updated_at":"2025-11-18T14:10:20.000Z","dependencies_parsed_at":"2025-06-24T07:40:49.493Z","dependency_job_id":null,"html_url":"https://github.com/gear-genomics/indigo","commit_stats":null,"previous_names":[],"tags_count":0,"template":false,"template_full_name":null,"purl":"pkg:github/gear-genomics/indigo","repository_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/gear-genomics%2Findigo","tags_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/gear-genomics%2Findigo/tags","releases_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/gear-genomics%2Findigo/releases","manifests_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/gear-genomics%2Findigo/manifests","owner_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners/gear-genomics","download_url":"https://codeload.github.com/gear-genomics/indigo/tar.gz/refs/heads/main","sbom_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/gear-genomics%2Findigo/sbom","scorecard":null,"host":{"name":"GitHub","url":"https://github.com","kind":"github","repositories_count":286080680,"owners_count":28530281,"icon_url":"https://github.com/github.png","version":null,"created_at":"2022-05-30T11:31:42.601Z","updated_at":"2026-01-18T00:39:45.795Z","status":"online","status_checked_at":"2026-01-18T02:00:07.578Z","response_time":98,"last_error":null,"robots_txt_status":"success","robots_txt_updated_at":"2025-07-24T06:49:26.215Z","robots_txt_url":"https://github.com/robots.txt","online":true,"can_crawl_api":true,"host_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub","repositories_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories","repository_names_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repository_names","owners_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners"}},"keywords":["alignment","alleles","chromatogram-traces","crispr-analysis","crispr-cas9","gear-genomics","indel-discovery","indels","indigo","pcr","pcr-products","sanger-chromatograms","sanger-sequencing","sequencing","variant-calling","variants"],"created_at":"2026-01-18T04:48:35.655Z","updated_at":"2026-01-18T04:48:35.754Z","avatar_url":"https://github.com/gear-genomics.png","language":"JavaScript","readme":"\u003cp align=\"center\"\u003e\n  \u003cimg height=\"100\" src=\"https://raw.githubusercontent.com/tobiasrausch/indigo/master/indigo.png\"\u003e\n\u003c/p\u003e\n\n[Indigo](https://www.gear-genomics.com/indigo) is a rapid single-nucleotide variant (SNV) and insertion/deletion (InDel) discovery method in Chromatogram traces obtained from Sanger sequencing of PCR products. It can separate a mutated and wildtype allele and aligns both alleles against a reference sequence or wildtype chromatogram. Indigo discovers mutations generated by genome editing tools such as CRISPR/Cas9 or TALENs.\n\nIndigo Online Method\n--------------------\n\n[Indigo](https://www.gear-genomics.com/indigo) can be run online as a web application at [https://www.gear-genomics.com/indigo](https://www.gear-genomics.com/indigo).\n","funding_links":[],"categories":[],"sub_categories":[],"project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fgear-genomics%2Findigo","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Fgear-genomics%2Findigo","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fgear-genomics%2Findigo/lists"}