{"id":24391639,"url":"https://github.com/jumitti/transfectionratioxpert","last_synced_at":"2025-09-29T22:31:00.417Z","repository":{"id":223446003,"uuid":"757495361","full_name":"Jumitti/TransfectionRatioXpert","owner":"Jumitti","description":"Just a Streamlit tool to calculate ratio of DNA for transfection","archived":false,"fork":false,"pushed_at":"2024-06-05T19:46:26.000Z","size":196,"stargazers_count":1,"open_issues_count":0,"forks_count":0,"subscribers_count":1,"default_branch":"main","last_synced_at":"2024-06-06T20:00:03.078Z","etag":null,"topics":["bioinfor","biology","python","science","streamlit","streamlit-application","streamlit-webapp","transfection"],"latest_commit_sha":null,"homepage":"https://transfectionratioxpert.streamlit.app/","language":"Python","has_issues":true,"has_wiki":null,"has_pages":null,"mirror_url":null,"source_name":null,"license":"mit","status":null,"scm":"git","pull_requests_enabled":true,"icon_url":"https://github.com/Jumitti.png","metadata":{"files":{"readme":"README.md","changelog":null,"contributing":null,"funding":null,"license":"LICENSE","code_of_conduct":null,"threat_model":null,"audit":null,"citation":null,"codeowners":null,"security":null,"support":null,"governance":null,"roadmap":null,"authors":null,"dei":null,"publiccode":null,"codemeta":null}},"created_at":"2024-02-14T16:02:45.000Z","updated_at":"2024-06-06T20:00:03.078Z","dependencies_parsed_at":"2024-06-05T19:29:19.034Z","dependency_job_id":null,"html_url":"https://github.com/Jumitti/TransfectionRatioXpert","commit_stats":null,"previous_names":["jumitti/transfectionratioxpert"],"tags_count":1,"template":false,"template_full_name":null,"repository_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Jumitti%2FTransfectionRatioXpert","tags_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Jumitti%2FTransfectionRatioXpert/tags","releases_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Jumitti%2FTransfectionRatioXpert/releases","manifests_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/Jumitti%2FTransfectionRatioXpert/manifests","owner_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners/Jumitti","download_url":"https://codeload.github.com/Jumitti/TransfectionRatioXpert/tar.gz/refs/heads/main","host":{"name":"GitHub","url":"https://github.com","kind":"github","repositories_count":234670022,"owners_count":18869120,"icon_url":"https://github.com/github.png","version":null,"created_at":"2022-05-30T11:31:42.601Z","updated_at":"2022-07-04T15:15:14.044Z","host_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub","repositories_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories","repository_names_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repository_names","owners_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners"}},"keywords":["bioinfor","biology","python","science","streamlit","streamlit-application","streamlit-webapp","transfection"],"created_at":"2025-01-19T17:18:32.986Z","updated_at":"2025-09-29T22:31:00.109Z","avatar_url":"https://github.com/Jumitti.png","language":"Python","funding_links":[],"categories":[],"sub_categories":[],"readme":"# 🧬 Transfection Ratio Xpert\n\n### ⚠ THE PROJECT HAS BEEN COMPILED IN [LABMASTER](https://github.com/Jumitti/labmaster)\n\n**🌐 Application is accessible [HERE](https://labmaster.streamlit.app/)**\n\n**🖥️ Standalone application for WINDOWS is accessible [HERE](https://github.com/Jumitti/TransfectionRatioXpert/releases)**\n\n**📄 Full documentation [HERE](https://jumitti.notion.site/Transfection-Ratio-Xpert-c25f10537e454529a4397925fe9e68c5?pvs=74)**\n\nCellular transfection is a fundamental technique in molecular biology for introducing nucleic acids, such as DNA or RNA, \ninto living cells. This method plays a crucial role in many areas of research, including cell biology, biotechnology and \ngene therapy. By enabling targeted genetic manipulation and the study of cellular mechanisms, cell transfection offers \npromising opportunities for the understanding of biological processes and the development of new therapies.\n\nTransfection efficiency often depends on several factors, including the type of cells used, the transfection method, \nand optimal ratios of nucleic acids to transfection agents. Among the most commonly used transfection agents are \nLipofectamine 2000, Lipofectamine 3000 from Invitrogen, and jetPRIME from Polyplus-transfection. These reagents are \ndesigned to enhance the uptake and delivery of nucleic acids into cells, thereby facilitating the transfection process.\n\n\n## About Transfection Ratio Xpert\nThis application aims to simplify the process of calculating the necessary ratios between DNA and transfection agents \nduring cell transfection. By providing a user-friendly interface and advanced calculation tools, it allows researchers \nand scientists to optimize their transfection experiments, explore different parameters and generate personalized \nexperimental conditions. Please note that this application is intended for calculation assistance purposes only and \nmay contain errors. We recommend that you independently verify the results obtained before making decisions based on them.\n\n## Transfection Ratio Xpert Features\n**Full documentation, protocols and troubleshooting [HERE](https://jumitti.notion.site/Transfection-Ratio-Xpert-c25f10537e454529a4397925fe9e68c5?pvs=74)**\n- Instant preparation of multiple transfection conditions, without number restrictions.\n- Support for co-transfection with multiple DNAs, without limits.\n- Total flexibility to adjust the amount of DNA to transfect.\n- Selection of the transfectant among Lipofectamine and jetPRIME, with ratio DNA/transfectant.\n- Adaptation to a wide range of plate formats, from 96-well to 150 mm culture vessel.\n- Efficient calculation of the number of wells required based on the specified transfection parameters.\n\n### Credit\nCreated by [Minniti Julien](https://github.com/Jumitti) under [MIT licence](https://github.com/Jumitti/TransfectionRatioXpert/blob/main/LICENSE)\n","project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fjumitti%2Ftransfectionratioxpert","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Fjumitti%2Ftransfectionratioxpert","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fjumitti%2Ftransfectionratioxpert/lists"}