{"id":19998760,"url":"https://github.com/quantixed/p062p035","last_synced_at":"2026-02-04T12:05:06.911Z","repository":{"id":242100499,"uuid":"614311089","full_name":"quantixed/p062p035","owner":"quantixed","description":"Data and code for manuscript p062p035 - Shelford, Burgess et al., 2025","archived":false,"fork":false,"pushed_at":"2025-02-20T09:17:36.000Z","size":4656,"stargazers_count":1,"open_issues_count":0,"forks_count":0,"subscribers_count":1,"default_branch":"main","last_synced_at":"2025-07-09T09:51:05.470Z","etag":null,"topics":["paper"],"latest_commit_sha":null,"homepage":"","language":"HTML","has_issues":true,"has_wiki":null,"has_pages":null,"mirror_url":null,"source_name":null,"license":"gpl-3.0","status":null,"scm":"git","pull_requests_enabled":true,"icon_url":"https://github.com/quantixed.png","metadata":{"files":{"readme":"README.md","changelog":null,"contributing":null,"funding":null,"license":"LICENSE","code_of_conduct":null,"threat_model":null,"audit":null,"citation":null,"codeowners":null,"security":null,"support":null,"governance":null,"roadmap":null,"authors":null,"dei":null,"publiccode":null,"codemeta":null,"zenodo":null}},"created_at":"2023-03-15T10:32:54.000Z","updated_at":"2025-06-25T07:12:14.000Z","dependencies_parsed_at":null,"dependency_job_id":"a9f76543-7416-4bd3-bc53-626c4af6ebd0","html_url":"https://github.com/quantixed/p062p035","commit_stats":null,"previous_names":["quantixed/p062p035"],"tags_count":0,"template":false,"template_full_name":null,"purl":"pkg:github/quantixed/p062p035","repository_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/quantixed%2Fp062p035","tags_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/quantixed%2Fp062p035/tags","releases_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/quantixed%2Fp062p035/releases","manifests_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/quantixed%2Fp062p035/manifests","owner_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners/quantixed","download_url":"https://codeload.github.com/quantixed/p062p035/tar.gz/refs/heads/main","sbom_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/quantixed%2Fp062p035/sbom","scorecard":null,"host":{"name":"GitHub","url":"https://github.com","kind":"github","repositories_count":286080680,"owners_count":29084088,"icon_url":"https://github.com/github.png","version":null,"created_at":"2022-05-30T11:31:42.601Z","updated_at":"2026-02-04T03:31:03.593Z","status":"ssl_error","status_checked_at":"2026-02-04T03:29:50.742Z","response_time":62,"last_error":"SSL_connect returned=1 errno=0 peeraddr=140.82.121.5:443 state=error: unexpected eof while reading","robots_txt_status":"success","robots_txt_updated_at":"2025-07-24T06:49:26.215Z","robots_txt_url":"https://github.com/robots.txt","online":false,"can_crawl_api":true,"host_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub","repositories_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories","repository_names_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repository_names","owners_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners"}},"keywords":["paper"],"created_at":"2024-11-13T05:09:25.096Z","updated_at":"2026-02-04T12:05:06.895Z","avatar_url":"https://github.com/quantixed.png","language":"HTML","readme":"# p062p035\n\nData and code for manuscript `p062p035`\n\n**Structural characterization and inhibition of the interaction between ch-TOG and TACC3**\n\nJames Shelford\\*, Selena G. Burgess\\*, Elena Rostkova, Mark W. Richards, Gabrielle Larocque, Josephina Sampson, Christian Tiede, Alistair J. Fielding, Tina Daviter, Darren C. Tomlinson, Antonio N. Calabrese, Mark Pfuhl\\*\\*, Richard Bayliss\\*\\* and Stephen J. Royle\\*\\*\n\n*J Cell Biol* (2025) 224: e202407002, doi: [10.1083/jcb.202407002](https://doi.org/10.1083/jcb.202407002).\n\n\n## Code and data for recreation of plots\n\n### Main Figures\n\n- mCherry-Affimer IP quantification - `IP_Quantification`\n- Spindle ch-TOG quantification - `Spindle_Quantification`\n- Spindle tubulin quantification - `gl_tub`\n- Pericentrin quantification - `Pericentrin_quant`\n- Centrin-pericentrin quantification - `Centrin-pericentrin`\n- ch-TOG mutant pericentrin - `chTOG_mut_pcnt`\n- Sankey diagram - `Sankey`\n- Mitotic timing - `MitoticTiming`\n\n\n### Supplementary Figures\n\n- 3D Mitotic spindle analytics - `SpindleAnalytics`\n- Affimer knocksideways - `JS155`\n- Clathrin/TACC3 quantification - `gl_clat`\n- PCNT foci with nocodazole - `gl_noc`\n\n### Notes\n\nThe R/Igor code can be executed with data placed into the `Data` folder of the RProject.\n\nFiji scripts for image analysis are included in the `Script` folder of the respective project.\n\nRaw data (images) are not included for space reasons, only text outputs from image analysis steps.\n\n## Development\n\nSome code in this repo was developed elsewhere or is still under development.\nThe version in this repo reflects the state at the time the figures were assembled.\n\n[`https://github.com/james-shelford/Image_Analysis_Projects`](https://github.com/james-shelford/Image_Analysis_Projects)\n\n[`https://github.com/james-shelford/SpindleAnalytics`](https://github.com/james-shelford/SpindleAnalytics)\n\n[`https://github.com/quantixed/MitoticTiming`](https://github.com/quantixed/MitoticTiming)\n\n## Contributions\n\nR Code and ImageJ Scripts written by James Shelford.\nCode review and small changes for publication by Stephen Royle.\nIgor code by Stephen Royle.\n","funding_links":[],"categories":[],"sub_categories":[],"project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fquantixed%2Fp062p035","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Fquantixed%2Fp062p035","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fquantixed%2Fp062p035/lists"}