{"id":28323492,"url":"https://github.com/rraadd88/dseq","last_synced_at":"2025-06-24T00:31:00.745Z","repository":{"id":223165295,"uuid":"618961002","full_name":"rraadd88/dseq","owner":"rraadd88","description":"dseq: Deep sequencing analysis in dataframes","archived":false,"fork":false,"pushed_at":"2024-02-18T16:34:03.000Z","size":1035,"stargazers_count":2,"open_issues_count":0,"forks_count":0,"subscribers_count":2,"default_branch":"master","last_synced_at":"2025-06-10T07:06:52.555Z","etag":null,"topics":["deep-mutational-scanning","deep-sequencing"],"latest_commit_sha":null,"homepage":"https://rraadd88.github.io/dseq/","language":"Jupyter Notebook","has_issues":true,"has_wiki":null,"has_pages":null,"mirror_url":null,"source_name":null,"license":"apache-2.0","status":null,"scm":"git","pull_requests_enabled":true,"icon_url":"https://github.com/rraadd88.png","metadata":{"files":{"readme":"README.md","changelog":null,"contributing":null,"funding":null,"license":"LICENSE","code_of_conduct":null,"threat_model":null,"audit":null,"citation":"CITATION.cff","codeowners":null,"security":null,"support":null,"governance":null,"roadmap":null,"authors":null,"dei":null,"publiccode":null,"codemeta":null,"zenodo":null}},"created_at":"2023-03-25T21:07:47.000Z","updated_at":"2024-10-28T19:23:27.000Z","dependencies_parsed_at":"2024-02-18T18:40:42.349Z","dependency_job_id":"ccd6ddbc-5eeb-4dbc-bf3a-e303afa8670e","html_url":"https://github.com/rraadd88/dseq","commit_stats":null,"previous_names":["rraadd88/dseq"],"tags_count":0,"template":false,"template_full_name":null,"purl":"pkg:github/rraadd88/dseq","repository_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/rraadd88%2Fdseq","tags_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/rraadd88%2Fdseq/tags","releases_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/rraadd88%2Fdseq/releases","manifests_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/rraadd88%2Fdseq/manifests","owner_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners/rraadd88","download_url":"https://codeload.github.com/rraadd88/dseq/tar.gz/refs/heads/master","sbom_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories/rraadd88%2Fdseq/sbom","host":{"name":"GitHub","url":"https://github.com","kind":"github","repositories_count":261582513,"owners_count":23180603,"icon_url":"https://github.com/github.png","version":null,"created_at":"2022-05-30T11:31:42.601Z","updated_at":"2022-07-04T15:15:14.044Z","host_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub","repositories_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repositories","repository_names_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/repository_names","owners_url":"https://repos.ecosyste.ms/api/v1/hosts/GitHub/owners"}},"keywords":["deep-mutational-scanning","deep-sequencing"],"created_at":"2025-05-25T16:14:19.876Z","updated_at":"2025-06-24T00:31:00.737Z","avatar_url":"https://github.com/rraadd88.png","language":"Jupyter Notebook","funding_links":[],"categories":[],"sub_categories":[],"readme":"# ``dseq`` \u003ca href=\"#\"\u003e\u003cimg src=\"https://openmoji.org/data/color/svg/1F9F1.svg\" align=\"right\" width=\"20%\"\u003e\u003c/a\u003e\n\nDeep sequencing analysis in dataframes\n\u003c!-- [![Contributors][contributors-shield]][contributors-url]\n[![Forks][forks-shield]][forks-url]\n[![Stargazers][stars-shield]][stars-url] --\u003e\n\u003ca href=\"\"\u003e[![PyPI](https://img.shields.io/pypi/v/dseq?style=for-the-badge)\n![Python](https://img.shields.io/pypi/pyversions/dseq?style=for-the-badge)](https://pypi.org/project/dseq)\u003c/a\u003e\n\u003ca href=\"\"\u003e[![build](https://img.shields.io/github/actions/workflow/status/rraadd88/dseq/test.yaml?style=for-the-badge)](https://github.com/rraadd88/dseq/actions/workflows/test.yaml)\u003c/a\u003e\n\u003ca href=\"\"\u003e[![Issues](https://img.shields.io/github/issues/rraadd88/dseq.svg?style=for-the-badge)](https://github.com/rraadd88/dseq/issues)\u003c/a\u003e\n\u003ca href=\"\"\u003e[![Downloads](https://img.shields.io/pypi/dm/dseq?style=for-the-badge)](https://pepy.tech/project/dseq)\u003c/a\u003e\n\u003ca href=\"\"\u003e[![GNU License](https://img.shields.io/github/license/rraadd88/dseq.svg?style=for-the-badge)](https://github.com/rraadd88/dseq/blob/master/LICENSE)\u003c/a\u003e\n\n\u003c!-- WARNING: THIS FILE WAS AUTOGENERATED! DO NOT EDIT! --\u003e\n\n## Install\n\n``` sh\npip install dseq\n```\n\n## How to use\n\n### Read the bam file as a pandas dataframe\n\n``` python\nfrom dseq.tools import to_df\ndf=to_df(\n    bam_path='inputs/test.bam', # path to the bam file\n    contig='14',                # i.e. reference name/ target\n    start=74846395,             # start position\n    end=74846545,               # end position\n    verbose=True,               # verbose\n)\ndf.head(1)\n```\n\n\u003cdiv\u003e\n\n\n|     | query id                                          | CIGAR | read position | reference position | reference | read |\n|-----|---------------------------------------------------|-------|---------------|--------------------|-----------|------|\n| 0   | M04377:211:000000000-D637H:1:1101:7912:5949::u... | 151M  | 0.0           | 74846395.0         | C         | C    |\n\n\u003c/div\u003e\n\n## How to calculate\n\n### Coverage per position (`QC`)\n\n``` python\ndata=df.dseq.get_coverage()\ndata.head(1)\n```\n\n\u003cdiv\u003e\n\n\n|     | reference position | reference | depth |\n|-----|--------------------|-----------|-------|\n| 0   | 74846395.0         | C         | 377   |\n\n\u003c/div\u003e\n\n``` python\n## plot\n_=data.plot.area(\n    x='reference position',\n    y='depth',\n    ylabel='depth',\n    ylim=(data['depth'].max()+10,0),\n    legend=False,\n)\n```\n\n\u003cimg src=\"index_files/figure-commonmark/cell-4-output-1.png\"\nclass=\"quarto-discovered-preview-image\" /\u003e\n\n### Number of reads per mutations (`QC`)\n\n``` python\ndata=df.dseq.get_counts_per_mutation()\ndata.head(1)\n```\n\n    4    1\n    dtype: int64\n\n``` python\n## plot\n_=data.plot.barh(xlabel='mutations',ylabel='reads')\n```\n\n![](index_files/figure-commonmark/cell-6-output-1.png)\n\n### Read depth per nucleotide mutations\n\n``` python\ndata=df.dseq.get_counts_per_nt_mutation()\ndata.head(1)\n```\n\n\u003cdiv\u003e\n\n\n|     | reference position | reference | read | depth |\n|-----|--------------------|-----------|------|-------|\n| 0   | 74846395.0         | C         | C    | NaN   |\n\n\u003c/div\u003e\n\n#### Mutation map\n\n``` python\ndata.dseq.get_map(value='depth',aggfunc='sum')\n```\n\n\n\n| reference position | 74846395.0 | 74846396.0 | 74846397.0 | 74846398.0 | 74846399.0 | 74846400.0 | 74846401.0 | 74846402.0 | 74846403.0 | 74846404.0 | 74846405.0 | 74846406.0 | 74846407.0 | 74846408.0 | 74846409.0 | 74846410.0 | 74846411.0 | 74846412.0 | 74846413.0 | 74846414.0 | 74846415.0 | 74846416.0 | 74846417.0 | 74846418.0 | 74846419.0 | 74846420.0 | 74846421.0 | 74846422.0 | 74846423.0 | 74846424.0 | 74846425.0 | 74846426.0 | 74846427.0 | 74846428.0 | 74846429.0 | 74846430.0 | 74846431.0 | 74846432.0 | 74846433.0 | 74846434.0 | 74846435.0 | 74846436.0 | 74846437.0 | 74846438.0 | 74846439.0 | 74846440.0 | 74846441.0 | 74846442.0 | 74846443.0 | 74846444.0 | 74846445.0 | 74846446.0 | 74846447.0 | 74846448.0 | 74846449.0 | 74846450.0 | 74846451.0 | 74846452.0 | 74846453.0 | 74846454.0 | 74846455.0 | 74846456.0 | 74846457.0 | 74846458.0 | 74846459.0 | 74846460.0 | 74846461.0 | 74846462.0 | 74846463.0 | 74846464.0 | 74846465.0 | 74846466.0 | 74846467.0 | 74846468.0 | 74846469.0 | 74846470.0 | 74846471.0 | 74846472.0 | 74846473.0 | 74846474.0 | 74846475.0 | 74846476.0 | 74846477.0 | 74846478.0 | 74846479.0 | 74846480.0 | 74846481.0 | 74846482.0 | 74846483.0 | 74846484.0 | 74846485.0 | 74846486.0 | 74846487.0 | 74846488.0 | 74846489.0 | 74846490.0 | 74846491.0 | 74846492.0 | 74846493.0 | 74846494.0 | 74846495.0 | 74846496.0 | 74846497.0 | 74846498.0 | 74846499.0 | 74846500.0 | 74846501.0 | 74846502.0 | 74846503.0 | 74846504.0 | 74846505.0 | 74846506.0 | 74846507.0 | 74846508.0 | 74846509.0 | 74846510.0 | 74846511.0 | 74846512.0 | 74846513.0 | 74846514.0 | 74846515.0 | 74846516.0 | 74846517.0 | 74846518.0 | 74846519.0 | 74846520.0 | 74846521.0 | 74846522.0 | 74846523.0 | 74846524.0 | 74846525.0 | 74846526.0 | 74846527.0 | 74846528.0 | 74846529.0 | 74846530.0 | 74846531.0 | 74846532.0 | 74846533.0 | 74846534.0 | 74846535.0 | 74846536.0 | 74846537.0 | 74846538.0 | 74846539.0 | 74846540.0 | 74846541.0 | 74846542.0 | 74846543.0 | 74846544.0 | 74846545.0 |\n|--------------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|\n| reference          | C          | C          | T          | A          | A          | G          | C          | C          | T          | T          | T          | A          | C          | G          | G          | T          | C          | A          | C          | T          | C          | A          | C          | C          | A          | C          | T          | C          | A          | C          | T          | C          | A          | C          | T          | C          | A          | C          | T          | C          | A          | C          | C          | C          | A          | G          | A          | G          | C          | A          | A          | C          | T          | T          | C          | C          | G          | G          | T          | C          | C          | T          | G          | C          | A          | A          | A          | C          | T          | C          | C          | C          | T          | T          | C          | A          | T          | A          | G          | T          | A          | A          | G          | T          | G          | C          | C          | C          | T          | A          | T          | A          | C          | A          | G          | G          | T          | G          | T          | A          | T          | A          | A          | T          | T          | A          | A          | A          | A          | A          | G          | A          | A          | A          | A          | T          | A          | T          | T          | T          | T          | A          | T          | A          | C          | C          | A          | T          | G          | G          | T          | T          | T          | T          | A          | C          | T          | G          | T          | A          | C          | C          | T          | C          | C          | A          | T          | G          | G          | T          | T          |\n| read               |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |\n| A                  | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 2          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          |\n| C                  | 0          | 0          | 2          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 2          | 0          | 1          | 0          | 2          | 0          | 0          | 0          | 0          | 2          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 3          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 3          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 3          | 0          | 0          | 0          | 1          | 0          | 0          | 2          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          |\n| G                  | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 3          | 0          | 0          | 0          | 1          | 0          | 0          | 2          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 0          | 0          | 0          | 0          | 1          | 0          | 2          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 1          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 22         | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 2          |\n| T                  | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 1          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 2          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 0          | 0          |\n\n#### Substitution map\n\n``` python\ndata.dseq.get_submap(value='depth',aggfunc='sum')\n```\n\n\n\n| reference | A   | C   | G   | T   |\n|-----------|-----|-----|-----|-----|\n| read      |     |     |     |     |\n| A         | 0   | 2   | 6   | 2   |\n| C         | 5   | 0   | 0   | 29  |\n| G         | 19  | 0   | 0   | 25  |\n| T         | 5   | 2   | 5   | 0   |\n\n### Read depth per codons\n\n``` python\ndata=df.dseq.get_counts_per_codon_mutation()\ndata.head(1)\n```\n\n\u003cdiv\u003e\n\n\n|     | reference position | codon reference | codon read | depth |\n|-----|--------------------|-----------------|------------|-------|\n| 0   | 74846395.0         | CCT             | CCC        | 2.0   |\n\n\u003c/div\u003e\n\n#### Mutation map\n\n``` python\ndata.dseq.get_map(value='depth',aggfunc='sum')\n```\n\n\n\n| reference position | 74846395.0 | 74846396.0 | 74846397.0 | 74846398.0 | 74846399.0 | 74846400.0 | 74846401.0 | 74846402.0 | 74846403.0 | 74846404.0 | 74846405.0 | 74846406.0 | 74846407.0 | 74846408.0 | 74846409.0 | 74846410.0 | 74846411.0 | 74846412.0 | 74846413.0 | 74846414.0 | 74846415.0 | 74846416.0 | 74846417.0 | 74846418.0 | 74846419.0 | 74846420.0 | 74846421.0 | 74846422.0 | 74846423.0 | 74846424.0 | 74846425.0 | 74846426.0 | 74846427.0 | 74846428.0 | 74846429.0 | 74846430.0 | 74846431.0 | 74846432.0 | 74846433.0 | 74846434.0 | 74846435.0 | 74846436.0 | 74846437.0 | 74846438.0 | 74846439.0 | 74846440.0 | 74846441.0 | 74846442.0 | 74846443.0 | 74846444.0 | 74846445.0 | 74846446.0 | 74846447.0 | 74846448.0 | 74846449.0 | 74846450.0 | 74846451.0 | 74846452.0 | 74846453.0 | 74846454.0 | 74846455.0 | 74846456.0 | 74846457.0 | 74846458.0 | 74846459.0 | 74846460.0 | 74846461.0 | 74846462.0 | 74846463.0 | 74846464.0 | 74846465.0 | 74846466.0 | 74846467.0 | 74846468.0 | 74846469.0 | 74846470.0 | 74846471.0 | 74846472.0 | 74846473.0 | 74846474.0 | 74846475.0 | 74846476.0 | 74846477.0 | 74846478.0 | 74846479.0 | 74846480.0 | 74846481.0 | 74846482.0 | 74846483.0 | 74846484.0 | 74846485.0 | 74846486.0 | 74846487.0 | 74846488.0 | 74846489.0 | 74846490.0 | 74846491.0 | 74846492.0 | 74846493.0 | 74846494.0 | 74846495.0 | 74846496.0 | 74846497.0 | 74846498.0 | 74846499.0 | 74846500.0 | 74846501.0 | 74846502.0 | 74846503.0 | 74846504.0 | 74846505.0 | 74846506.0 | 74846507.0 | 74846508.0 | 74846509.0 | 74846510.0 | 74846511.0 | 74846512.0 | 74846513.0 | 74846514.0 | 74846515.0 | 74846516.0 | 74846517.0 | 74846518.0 | 74846519.0 | 74846520.0 | 74846521.0 | 74846522.0 | 74846523.0 | 74846524.0 | 74846525.0 | 74846526.0 | 74846527.0 | 74846528.0 | 74846529.0 | 74846530.0 | 74846531.0 | 74846532.0 | 74846533.0 | 74846534.0 | 74846535.0 | 74846536.0 | 74846537.0 | 74846538.0 | 74846539.0 | 74846540.0 | 74846541.0 | 74846542.0 | 74846543.0 | 74846544.0 |\n|--------------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|------------|\n| codon reference    | CCT        | CCT        | CCT        | AAG        | AAG        | AAG        | CCT        | CCT        | CCT        | TTA        | TTA        | TTA        | CGG        | CGG        | CGG        | TCA        | TCA        | TCA        | CTC        | CTC        | CTC        | ACC        | ACC        | ACC        | ACT        | ACT        | ACT        | CAC        | CAC        | CAC        | TCA        | TCA        | TCA        | CTC        | CTC        | CTC        | ACT        | ACT        | ACT        | CAC        | CAC        | CAC        | CCA        | CCA        | CCA        | GAG        | GAG        | GAG        | CAA        | CAA        | CAA        | CTT        | CTT        | CTT        | CCG        | CCG        | CCG        | GTC        | GTC        | GTC        | CTG        | CTG        | CTG        | CAA        | CAA        | CAA        | ACT        | ACT        | ACT        | CCC        | CCC        | CCC        | TTC        | TTC        | TTC        | ATA        | ATA        | ATA        | GTA        | GTA        | GTA        | AGT        | AGT        | AGT        | GCC        | GCC        | GCC        | CTA        | CTA        | CTA        | TAC        | TAC        | TAC        | AGG        | AGG        | AGG        | TGT        | TGT        | TGT        | ATA        | ATA        | ATA        | ATT        | ATT        | ATT        | AAA        | AAA        | AAA        | AAG        | AAG        | AAG        | AAA        | AAA        | AAA        | ATA        | ATA        | ATA        | TTT        | TTT        | TTT        | TAT        | TAT        | TAT        | ACC        | ACC        | ACC        | ATG        | ATG        | ATG        | GTT        | GTT        | GTT        | TTA        | TTA        | TTA        | CTG        | CTG        | CTG        | TAC        | TAC        | TAC        | CTC        | CTC        | CTC        | CAT        | CAT        | CAT        | GGT        | GGT        | GGT        |\n| codon read         |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |            |\n| AAA                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| AAC                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| AAG                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| ACA                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| ACC                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 2          | 2          | 2          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 3          | 3          | 3          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| ACT                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 2          | 2          | 2          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| AGA                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| AGC                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| AGG                | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          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| 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| AGT                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 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         | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 1          | 1          | 1          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          |\n| ATC                | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0          | 0   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