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href=\"https://orcid.org/0000-0001-9834-9857\"\u003ehttps://orcid.org/0000-0001-9834-9857\u003c/a\u003e)\u003c/p\u003e\n\u003cp\u003e\u0026#x8907;\u0026#x6570;\u0026#x500B;\u0026#x4F53;\u0026#x304B;\u0026#x3089;\u0026#x4E00;\u0026#x62EC;\u0026#x3067;\u0026#x62BD;\u0026#x51FA;\u0026#x3055;\u0026#x308C;\u0026#x305F; DNA \u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#xFF08;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF; DNA \u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#xFF09;\u0026#x306B;\u0026#x5BFE;\u0026#x3059;\u0026#x308B;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x306E; \u0026#x394;\u0026#x394;Cq 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English\u003c/a\u003e\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u0026#x3057;\u0026#x3070;\u0026#x3089;\u0026#x304F; freqpcr \u0026#x306E;\u0026#x539F;\u0026#x7406;\u0026#x8AAC;\u0026#x660E;\u0026#x306A;\u0026#x306E;\u0026#x3067;\u0026#x3001;\u0026#x3068;\u0026#x308A;\u0026#x3042;\u0026#x3048;\u0026#x305A;\u0026#x52D5;\u0026#x304B;\u0026#x3057;\u0026#x3066;\u0026#x307F;\u0026#x305F;\u0026#x3044;\u0026#x4EBA;\u0026#x306F; \u003cstrong\u003e\u003ca href=\"#%E3%82%A4%E3%83%B3%E3%82%B9%E3%83%88%E3%83%BC%E3%83%AB\"\u003e\u0026#x30A4;\u0026#x30F3;\u0026#x30B9;\u0026#x30C8;\u0026#x30FC;\u0026#x30EB;\u003c/a\u003e\u003c/strong\u003e \u0026#x3078;\u0026#x9032;\u0026#x3093;\u0026#x3067;\u0026#x304F;\u0026#x3060;\u0026#x3055;\u0026#x3044;\u0026#x3002;\u003c/p\u003e\n\u003ch1 class=\"mume-header\" id=\"%E7%89%B9%E5%BE%B4\"\u003e\u0026#x7279;\u0026#x5FB4;\u003c/h1\u003e\n\n\u003cp\u003e\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306B;\u0026#x6E96;\u0026#x62E0;\u0026#x3057;\u0026#x305F;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304D;\u0026#x3001;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#xFF08;population allele frequency\u0026#xFF09;\u0026#x3092;\u0026#x533A;\u0026#x9593;\u0026#x63A8;\u0026#x5B9A;\u0026#x3059;\u0026#x308B; R \u0026#x30D1;\u0026#x30C3;\u0026#x30B1;\u0026#x30FC;\u0026#x30B8;\u0026#x3002;\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u0026#x52D5;\u0026#x7269;\u0026#x3084;\u0026#x690D;\u0026#x7269;\u0026#x306E;\u0026#x7279;\u0026#x5B9A;\u0026#x306E;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x554F;\u0026#x984C;\u0026#x3068;\u0026#x3059;\u0026#x308B;\u0026#x7279;\u0026#x5B9A;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#xFF08;\u0026#x5BFE;\u0026#x7ACB;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#xFF09;\u0026#x304C;\u0026#x305D;\u0026#x308C;\u0026#x4EE5;\u0026#x5916;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306B;\u0026#x5BFE;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x30EC;\u0026#x30D9;\u0026#x30EB;\u0026#x3067;\u0026#x3069;\u0026#x306E;\u0026#x7A0B;\u0026#x5EA6;\u0026#x306E;\u0026#x5272;\u0026#x5408;\u0026#x3067;\u0026#x5B58;\u0026#x5728;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x304B;\u0026#x3092;\u0026#x77E5;\u0026#x308A;\u0026#x305F;\u0026#x3044;\u0026#x3002;\u003c/li\u003e\n\u003cli\u003e\u0026#x5BFE;\u0026#x8C61;\u0026#x306E;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x8907;\u0026#x6570;\u0026#x306E;\u0026#x500B;\u0026#x4F53;\u0026#x3092;\u0026#x4E00;\u0026#x62EC;\u0026#x3057;\u0026#x3066;\u0026#x62BD;\u0026#x51FA;\u0026#x3055;\u0026#x308C;\u0026#x305F; DNA \u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#xFF08;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF; DNA \u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#xFF09;\u0026#x304C;\u0026#x8907;\u0026#x6570;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u003c/li\u003e\n\u003cli\u003e\u0026#x5404;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304F;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x304C;\u0026#x884C;\u0026#x308F;\u0026#x308C;\u0026#x3001;Cq \u0026#x5024;\u0026#x306E;\u0026#x7D44;\u0026#x304C;\u0026#x5229;\u0026#x7528;\u0026#x53EF;\u0026#x80FD;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/li\u003e\n\u003cli\u003e\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3054;\u0026#x3068;\u0026#x306B;\u0026#x3001;Cq \u0026#x5024;\u0026#x306E;\u0026#x7D44;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x3066;\u0026#x8A08;\u0026#x7B97;\u0026#x3055;\u0026#x308C;\u0026#x305F; \u0026#x394;\u0026#x394;Cq \u0026#x5024;\u0026#x304C;\u0026#x3001;\u0026#x305D;\u0026#x306E;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x304A;\u0026#x3051;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x6307;\u0026#x6A19;\u0026#x3068;\u0026#x306A;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x306A; PCR \u0026#x5206;\u0026#x6790;\u0026#x624B;\u0026#x6CD5;\u0026#xFF08;\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#xFF09;\u0026#x304C;\u0026#x7528;\u0026#x3044;\u0026#x3089;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u0026#x4E0A;\u0026#x8A18;\u0026#x306E;\u0026#x8981;\u0026#x4EF6;\u0026#x3067;\u0026#x3001;\u0026#x5404;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x4F55;\u0026#x500B;\u0026#x4F53;\u0026#x5206;\u0026#x306E; DNA \u0026#x304C;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x304B;\u0026#xFF08;N\u0026#xFF09;\u0026#x3068;\u0026#x3001;\u0026#x5404;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x6E2C;\u0026#x5B9A;\u0026#x3055;\u0026#x308C;\u0026#x305F; Cq \u0026#x5024;\u0026#x306E;\u0026#x7D44;\u0026#xFF08;\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306A;\u0026#x306E;\u0026#x3067; 4 \u0026#x3064;\u0026#x305A;\u0026#x3064;\u0026#x3042;\u0026#x308B;\u0026#x203B;\u0026#xFF09;\u0026#x3092;\u0026#x5165;\u0026#x529B;\u0026#x30C7;\u0026#x30FC;\u0026#x30BF;\u0026#x306B;\u0026#x53D6;\u0026#x308A;\u0026#x3001;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x30EC;\u0026#x30D9;\u0026#x30EB;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x6700;\u0026#x5C24;\u0026#x63A8;\u0026#x5B9A;\u0026#x5024;\u0026#x3001;\u0026#x304A;\u0026#x3088;\u0026#x3073;\u0026#x305D;\u0026#x306E;\u0026#x6F38;\u0026#x8FD1;\u0026#x4FE1;\u0026#x983C;\u0026#x533A;\u0026#x9593;\u0026#x3092;\u0026#x63A8;\u0026#x5B9A;\u0026#x3059;\u0026#x308B;\u0026#x30C4;\u0026#x30FC;\u0026#x30EB;\u0026#xFF08;\u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#xFF09;\u0026#x3092;\u0026#x63D0;\u0026#x4F9B;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x63A8;\u0026#x5B9A;\u0026#x306B;\u0026#x5148;\u0026#x7ACB;\u0026#x3063;\u0026#x3066;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x6642;\u0026#x306E;\u0026#x5897;\u0026#x5E45;\u0026#x52B9;\u0026#x7387;\u0026#x306A;\u0026#x3069;\u0026#x306E;\u0026#x3001;\u0026#x88DC;\u0026#x52A9;\u0026#x7684;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x304C;\u0026#x5FC5;\u0026#x8981;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x6301;\u0026#x3064;\u0026#x500B;\u0026#x4F53;\u0026#x3068;\u0026#x3001;\u0026#x305D;\u0026#x308C;\u0026#x4EE5;\u0026#x5916;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x6301;\u0026#x3064;\u0026#x500B;\u0026#x4F53;\u0026#x306B;\u0026#x7531;\u0026#x6765;\u0026#x3059;\u0026#x308B; DNA \u0026#x62BD;\u0026#x51FA;\u0026#x7269;\u0026#x3092;\u0026#x3001;\u0026#x65E2;\u0026#x77E5;\u0026#x983B;\u0026#x5EA6;\u0026#x3067;\u0026#x6DF7;\u0026#x5408;\u0026#x3057;\u0026#x305F;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x6EB6;\u0026#x6DB2;\u0026#x3092;\u0026#x4F7F;\u0026#x3063;\u0026#x3066;\u0026#x3001;\u0026#x88DC;\u0026#x52A9;\u0026#x7684;\u0026#x306A;\u0026#x5B9F;\u0026#x9A13;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x3092;\u0026#x4E8B;\u0026#x524D;\u0026#x306B;\u0026#x63A8;\u0026#x5B9A;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x95A2;\u0026#x6570;\u0026#xFF08;\u003ccode\u003eknownqpcr()\u003c/code\u003e \u0026#x304A;\u0026#x3088;\u0026#x3073; \u003ccode\u003eknownqpcr_unpaired()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#xFF09;\u0026#x3082;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u0026#x203B;\u0026#x30D0;\u0026#x30FC;\u0026#x30B8;\u0026#x30E7;\u0026#x30F3; 0.4.0 \u0026#x4EE5;\u0026#x964D;\u0026#x3067;\u0026#x306F; 2 \u0026#x3064;\u0026#x306E; Cq \u0026#x6E2C;\u0026#x5B9A;\u0026#x5024;\u0026#x306E;\u0026#x7D44;\u0026#xFF08;\u0026#x394;Cq \u0026#x6CD5;\u0026#xFF09;\u0026#x3067;\u0026#x3082;\u0026#x52D5;\u0026#x4F5C;\u0026#x3059;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x306B;\u0026#x306A;\u0026#x3063;\u0026#x305F;\u0026#x3002;\u003c/p\u003e\n\u003c/blockquote\u003e\n\u003ch1 class=\"mume-header\" id=\"%E5%8B%95%E4%BD%9C%E5%8E%9F%E7%90%86\"\u003e\u0026#x52D5;\u0026#x4F5C;\u0026#x539F;\u0026#x7406;\u003c/h1\u003e\n\n\u003ch2 class=\"mume-header\" id=\"%E8%A4%87%E6%95%B0%E5%80%8B%E4%BD%93%E3%81%AE%E6%B7%B7%E5%90%88-dna-%E6%BA%B6%E6%B6%B2%E3%81%AB%E5%90%AB%E3%81%BE%E3%82%8C%E3%82%8B%E3%82%A2%E3%83%AA%E3%83%AB%E5%AD%98%E5%9C%A8%E6%AF%94%E3%81%AE%E3%83%A2%E3%83%87%E3%83%AB%E5%8C%96\"\u003e\u0026#x8907;\u0026#x6570;\u0026#x500B;\u0026#x4F53;\u0026#x306E;\u0026#x6DF7;\u0026#x5408; DNA \u0026#x6EB6;\u0026#x6DB2;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x5B58;\u0026#x5728;\u0026#x6BD4;\u0026#x306E;\u0026#x30E2;\u0026#x30C7;\u0026#x30EB;\u0026#x5316;\u003c/h2\u003e\n\n\u003cp\u003e\u0026#x524D;\u0026#x63D0;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x3068;\u0026#x3059;\u0026#x308B;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x672A;\u0026#x77E5;\u0026#x983B;\u0026#x5EA6; p : (1 - p) \u0026#x3067; 2 \u0026#x7A2E;\u0026#x985E;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x542B;\u0026#x3080;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x304B;\u0026#x3089;\u0026#x3001;\u0026#x8907;\u0026#x6570;\u0026#x306E;\u0026#x500B;\u0026#x4F53;\u0026#x3092;\u0026#x30E9;\u0026#x30F3;\u0026#x30C0;\u0026#x30E0;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EA;\u0026#x30F3;\u0026#x30B0;\u0026#x3057;\u0026#x305F;\u0026#x3068;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x306A;\u0026#x304A;\u0026#x300C;2 \u0026#x7A2E;\u0026#x985E;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x300D;\u0026#x306F;\u0026#x300C;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x3068;\u0026#x305D;\u0026#x308C;\u0026#x4EE5;\u0026#x5916;\u0026#x306E;\u0026#x5168;\u0026#x7A2E;\u0026#x985E;\u0026#x300D;\u0026#x3067;\u0026#x3082;\u0026#x3044;\u0026#x3044;\u0026#x3002;PCR \u0026#x5206;\u0026#x6790;\u0026#x306B;\u0026#x7528;\u0026#x3044;\u0026#x308B;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x306F;\u0026#x3001;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x306B;\u0026#x5897;\u0026#x5E45;\u0026#x3059;\u0026#x308B;\u0026#x304B;\u0026#x3001;\u0026#x3042;\u0026#x308B;\u0026#x3044;\u0026#x306F;\u0026#x5F8C;\u0026#x8FF0;\u0026#x3059;\u0026#x308B;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x306B;\u0026#x3088;\u0026#x308A;\u0026#x3001;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x4EE5;\u0026#x5916;\u0026#x304C;\u0026#x5206;\u0026#x89E3;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x306B;\u0026#x8A2D;\u0026#x8A08;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x305F;\u0026#x3068;\u0026#x3048;\u0026#x3070;\u0026#x30D5;\u0026#x30A7;\u0026#x30ED;\u0026#x30E2;\u0026#x30F3;\u0026#x3092;\u0026#x8A98;\u0026#x5F15;\u0026#x6E90;\u0026#x3068;\u0026#x3057;\u0026#x305F;\u0026#x30C8;\u0026#x30E9;\u0026#x30C3;\u0026#x30D7;\u0026#x3067;\u0026#x3001;n \u0026#x500B;\u0026#x4F53;\u0026#x306E;\u0026#x5BB3;\u0026#x866B;\u0026#xFF08;\u0026#x305F;\u0026#x3060;\u0026#x3057;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x3092;\u0026#x4EEE;\u0026#x5B9A;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#xFF09;\u0026#x3092;\u0026#x63A1;\u0026#x96C6;\u0026#x3057;\u0026#x3001;\u0026#x3046;\u0026#x3061; m \u0026#x500B;\u0026#x4F53;\u0026#x304C;\u0026#x85AC;\u0026#x5264;\u0026#x62B5;\u0026#x6297;\u0026#x6027;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#xFF08;R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#xFF09;\u0026#x3092;\u0026#x3001;\u0026#x6B8B;\u0026#x308A; n - m \u0026#x500B;\u0026#x4F53;\u0026#x304C;\u0026#x611F;\u0026#x53D7;\u0026#x6027;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#xFF08;S \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#xFF09;\u0026#x3092;\u0026#x6301;\u0026#x3064;\u0026#x72B6;\u0026#x6CC1;\u0026#x3092;\u0026#x8003;\u0026#x3048;\u0026#x308B;\u0026#xFF08;Figure 1\u0026#xFF09;\u0026#x3002;\u0026#x3053;\u0026#x306E;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EA;\u0026#x30F3;\u0026#x30B0;\u0026#x904E;\u0026#x7A0B;\u0026#x306F;\u0026#x4E8C;\u0026#x9805;\u0026#x5206;\u0026#x5E03; m ~ Bin(n, p) \u0026#x3067;\u0026#x8868;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp align=\"center\"\u003e\n\u003cimg src=\"./vignettes/figures/freqpcr_vignette_fig01.png\" style=\"width: 75%; height: 75%\" alt=\"Figure 1. \u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EA;\u0026#x30F3;\u0026#x30B0;\u0026#x30C7;\u0026#x30B6;\u0026#x30A4;\u0026#x30F3;\"\u003e\n\u003c/p\u003e\n\u003cblockquote\u003e\n\u003cp\u003eFigure 1. \u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306E;\u0026#x751F;\u0026#x6210;\u0026#x904E;\u0026#x7A0B;\u0026#x3002;\u0026#x672A;\u0026#x77E5;\u0026#x983B;\u0026#x5EA6; p : (1 - p) \u0026#x3067; 2 \u0026#x7A2E;\u0026#x985E;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3001;\u0026#x305F;\u0026#x3068;\u0026#x3048;\u0026#x3070; R\u0026#xFF08;\u0026#x85AC;\u0026#x5264;\u0026#x62B5;\u0026#x6297;\u0026#x6027;\u0026#xFF09;\u0026#x3068; S\u0026#xFF08;\u0026#x611F;\u0026#x53D7;\u0026#x6027;\u0026#xFF09;\u0026#x3092;\u0026#x542B;\u0026#x3080;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x3092;\u0026#x5BFE;\u0026#x8C61;\u0026#x306B;\u0026#x3001;\u0026#x30C8;\u0026#x30E9;\u0026#x30C3;\u0026#x30D7;\u0026#x3092;\u0026#x4ED5;\u0026#x639B;\u0026#x3051;\u0026#x3066;\u0026#x500B;\u0026#x4F53;\u0026#x3092;\u0026#x63A1;\u0026#x96C6;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x3042;\u0026#x308B;\u0026#x30C8;\u0026#x30E9;\u0026#x30C3;\u0026#x30D7;\u0026#x3067; n \u0026#x500B;\u0026#x4F53;\u0026#x304C;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x305F;\u0026#x3068;\u0026#x304D;\u0026#x3001;\u0026#x3053;\u0026#x308C;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B; R \u0026#x500B;\u0026#x4F53;\u0026#x306E;\u0026#x6570;\u0026#x306F; m ~ Bin(n, p) \u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x78BA;\u0026#x7387;\u0026#x5909;\u0026#x6570;\u0026#x3067;\u0026#x8868;\u0026#x73FE;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x500B;\u0026#x4F53;\u0026#x5358;\u0026#x4F4D;\u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x304F;\u0026#x30C8;\u0026#x30E9;\u0026#x30C3;\u0026#x30D7;\u0026#x5358;\u0026#x4F4D;\u0026#x3067; DNA \u0026#x3092;\u0026#x4E00;\u0026#x62EC;\u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x3066;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x3092;\u0026#x884C;\u0026#x3046;\u0026#x3068;\u0026#x3001;\u0026#x500B;\u0026#x4F53;\u0026#x306E;\u0026#x5206;\u0026#x96E2;\u0026#x6BD4; m : (n - m) \u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x304F;\u0026#x3001;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x304A;\u0026#x3051;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB; DNA \u0026#x306E;\u0026#x542B;\u0026#x6709;\u0026#x91CF;\u0026#x3067;\u0026#x3042;\u0026#x308B; X_R, X_S \u0026#x304C;\u0026#x30C7;\u0026#x30FC;\u0026#x30BF;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x89B3;\u0026#x6E2C;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x305D;\u0026#x3053;\u0026#x3067; freqpcr \u0026#x3067;\u0026#x306F; p \u0026#x3068; X_R, X_S \u0026#x306E;\u0026#x95A2;\u0026#x4FC2;\u0026#x3092;\u0026#x3001;m \u0026#x3092;\u0026#x6F5C;\u0026#x5728;\u0026#x5909;\u0026#x6570;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x4ECB;\u0026#x3057;\u0026#x3064;\u0026#x3064;\u0026#x30E2;\u0026#x30C7;\u0026#x30EB;\u0026#x5316;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003c/blockquote\u003e\n\u003cp\u003e\u0026#x4EEE;\u0026#x306B;\u0026#x500B;\u0026#x4F53;\u0026#x5225;\u0026#x306B;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x8A3A;\u0026#x65AD;\u0026#x3059;\u0026#x308C;\u0026#x3070; m \u0026#x306E;\u0026#x5024;\u0026#x304C;\u0026#x76F4;\u0026#x63A5;\u0026#x6C42;\u0026#x307E;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x3001;\u0026#x983B;\u0026#x5EA6; p \u0026#x306E;\u0026#x63A8;\u0026#x5B9A;\u0026#x5024;\u0026#x3084;\u0026#x4FE1;\u0026#x983C;\u0026#x533A;\u0026#x9593;\u0026#x3092;\u0026#x4E8C;\u0026#x9805;\u0026#x5206;\u0026#x5E03;\u0026#x304B;\u0026#x3089;\u0026#x8A08;\u0026#x7B97;\u0026#x3067;\u0026#x304D;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x308C;\u0026#x304C;\u0026#x52B4;\u0026#x529B;\u0026#x7684;\u0026#x306B;\u0026#x53EF;\u0026#x80FD;\u0026#x306A;\u0026#x3089;\u0026#x3070;\u0026#x3001;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x3092;\u0026#x884C;\u0026#x3063;\u0026#x3066; \u003ccode\u003efreqpcr\u003c/code\u003e \u0026#x3092;\u0026#x4F7F;\u0026#x3046;\u0026#x5FC5;\u0026#x8981;\u0026#x306F;\u0026#x7279;\u0026#x6BB5;\u0026#x306A;\u0026#x3044;\u0026#x3002;\n\u0026#x3057;\u0026#x304B;\u0026#x3057; p \u0026#x304C;\u0026#x6975;\u0026#x3081;\u0026#x3066;\u0026#x5C0F;\u0026#x3055;\u0026#x3044;\u0026#x3053;\u0026#x3068;\u0026#x304C;\u0026#x60F3;\u0026#x5B9A;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x5834;\u0026#x5408;\u0026#x3001;\u0026#x305F;\u0026#x3068;\u0026#x3048;\u0026#x3070;\u0026#x6BBA;\u0026#x866B;\u0026#x5264;\u0026#x62B5;\u0026#x6297;\u0026#x6027;\u0026#x306E;\u0026#x767A;\u0026#x9054;\u0026#x521D;\u0026#x671F;\u0026#x6BB5;\u0026#x968E;\u0026#x306B;\u0026#x304A;\u0026#x3051;\u0026#x308B; R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x691C;\u0026#x51FA;\u0026#x3084;\u0026#x3001;\u0026#x7A40;\u0026#x7269;\u0026#x306E;\u0026#x8F38;\u0026#x5165;\u0026#x691C;\u0026#x75AB;\u0026#x306B;\u0026#x304A;\u0026#x3051;\u0026#x308B;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x7D44;\u0026#x63DB;\u0026#x3048;\u0026#x7A2E;\u0026#x5B50;\u0026#x306E;\u0026#x6DF7;\u0026#x5165;\u0026#x7387;\u0026#x8ABF;\u0026#x67FB;\u0026#x306A;\u0026#x3069;\u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x5341;\u0026#x5206;\u0026#x306B;\u0026#x5927;\u0026#x304D;\u0026#x306A;\u0026#x63A1;\u0026#x96C6;\u0026#x6570; n \u0026#x3092;\u0026#x78BA;\u0026#x4FDD;\u0026#x3057;\u0026#x306A;\u0026#x3044;\u0026#x3068;\u0026#x3001;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x5168;\u0026#x304F;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x304C;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x306A;\u0026#x3044;\u0026#xFF08;\u0026#x6709;\u0026#x52B9;\u0026#x306A;\u0026#x63A8;\u0026#x5B9A;\u0026#x5024;\u0026#x304C;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x306A;\u0026#x3044;\u0026#xFF09;\u0026#x53EF;\u0026#x80FD;\u0026#x6027;\u0026#x304C;\u0026#x9AD8;\u0026#x304F;\u0026#x306A;\u0026#x308B;\u0026#x3002;\u0026#x5FC5;\u0026#x8981;\u0026#x306A;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x30B5;\u0026#x30A4;\u0026#x30BA;\u0026#x304C;\u0026#x6570;\u0026#x767E;\u0026#x306B;\u0026#x53CA;\u0026#x3076;\u0026#x5834;\u0026#x5408;\u0026#x3001;\u0026#x500B;\u0026#x4F53;\u0026#x5225;\u0026#x306B; DNA \u0026#x3092;\u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x3066; PCR \u0026#x3067;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x8A3A;\u0026#x65AD;\u0026#x3092;\u0026#x884C;\u0026#x3046;\u0026#x624B;\u0026#x9593;\u0026#x304C;\u0026#x5897;\u0026#x5927;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x591A;\u0026#x6570;\u0026#x306E;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x304B;\u0026#x3089;\u0026#x7C21;\u0026#x6613;\u0026#x306B;\u0026#x4F4E;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x691C;\u0026#x51FA;\u0026#x3059;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x306B;\u0026#x3001;\u0026#x30B0;\u0026#x30EB;\u0026#x30FC;\u0026#x30D7;\u0026#x30C6;\u0026#x30B9;\u0026#x30C8;\u0026#xFF08;group testing\u0026#xFF09;\u0026#x3068;\u0026#x547C;\u0026#x3070;\u0026#x308C;\u0026#x308B;\u0026#x7D71;\u0026#x8A08;\u0026#x624B;\u0026#x6CD5;\u0026#x304C;\u0026#x5F93;\u0026#x6765;\u0026#x304B;\u0026#x3089;\u0026#x7528;\u0026#x3044;\u0026#x3089;\u0026#x308C;\u0026#x3066;\u0026#x304D;\u0026#x305F;\u0026#x3002;\u0026#x307E;\u0026#x305A;\u0026#x8907;\u0026#x6570;\u0026#x500B;\u0026#x4F53;\u0026#x304B;\u0026#x3089;\u0026#x4E00;\u0026#x62EC;\u0026#x3067; DNA \u0026#x3092;\u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#xFF08;bulk sample\u0026#xFF09;\u0026#x3068;\u0026#x547C;\u0026#x3070;\u0026#x308C;\u0026#x308B;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x6EB6;\u0026#x6DB2;\u0026#x3092;\u0026#x4F5C;\u0026#x308B;\u0026#x3002;\u0026#x901A;\u0026#x5E38;\u0026#x306E;\u0026#x30B0;\u0026#x30EB;\u0026#x30FC;\u0026#x30D7;\u0026#x30C6;\u0026#x30B9;\u0026#x30C8;\u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x5897;\u0026#x5E45;\u0026#x3059;\u0026#x308B; PCR \u0026#x53CD;\u0026#x5FDC;\u0026#x3092;\u0026#x884C;\u0026#x3044;\u0026#x3001;\u0026#x5404;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x5C11;\u0026#x306A;\u0026#x304F;\u0026#x3068;\u0026#x3082; 1 \u0026#x500B;\u0026#x4F53;\u0026#x306F;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x304C;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#xFF08;\u0026#x967D;\u0026#x6027;\u0026#xFF09;\u0026#x304B;\u0026#x3001;\u0026#x5168;\u0026#x304F;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x305A; PCR \u0026#x5897;\u0026#x5E45;\u0026#x3055;\u0026#x308C;\u0026#x306A;\u0026#x3044;\u0026#xFF08;\u0026#x9670;\u0026#x6027;\u0026#xFF09;\u0026#x304B;\u0026#x3092;\u0026#x5224;\u0026#x5B9A;\u0026#x3057;\u0026#x305F;\u0026#x7D50;\u0026#x679C;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u0026#x8907;\u0026#x6570;\u0026#x306E;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x967D;\u0026#x6027;\u0026#xFF0F;\u0026#x9670;\u0026#x6027;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x5272;\u0026#x5408;\u0026#x3092;\u0026#x6C42;\u0026#x3081;\u0026#x3001;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6; p \u0026#x306E;\u0026#x4FE1;\u0026#x983C;\u0026#x533A;\u0026#x9593;\u0026#x306E;\u0026#x4E0A;\u0026#x9650;\u0026#x3092;\u0026#x8A08;\u0026#x7B97;\u0026#x3059;\u0026#x308B;\u0026#x7D71;\u0026#x8A08;\u0026#x624B;\u0026#x6CD5;\u0026#x304C;\u0026#x3001;\u0026#x5E7E;\u0026#x3064;\u0026#x304B;\u0026#x306E;\u0026#x5148;\u0026#x884C;\u0026#x7814;\u0026#x7A76;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x63D0;\u0026#x5531;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#xFF08;\u0026#x305F;\u0026#x3068;\u0026#x3048;\u0026#x3070; Yamamura and Hino 2007\u0026#xFF09;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x4E00;\u0026#x65B9; \u003ccode\u003efreqpcr\u003c/code\u003e \u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x5404;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x3092;\u0026#x884C;\u0026#x3044;\u0026#x3001;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3054;\u0026#x3068;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B; DNA \u0026#x91CF;\u0026#xFF08;Figure 1\u0026#xFF1A;\u0026#x5909;\u0026#x6570; X_R, X_S\u0026#xFF09;\u0026#x3092;\u0026#x5B9A;\u0026#x91CF;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB; DNA \u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x306E;\u0026#x6307;\u0026#x6A19;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x5F93;\u0026#x6765;\u0026#x3088;\u0026#x308A;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x3067;\u0026#x7528;\u0026#x3044;\u0026#x3089;\u0026#x308C;\u0026#x3066;\u0026#x304D;\u0026#x305F; \u0026#x394;\u0026#x394;Cq \u0026#x5024;\u0026#x306E;\u0026#x6982;\u0026#x5FF5;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x3001;\u0026#x305D;\u0026#x308C;\u0026#x3089;\u0026#x306E;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x304C;\u0026#x7531;\u0026#x6765;\u0026#x3059;\u0026#x308B;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x63A8;\u0026#x5B9A;\u0026#x5024;\u0026#x3001;\u0026#x304A;\u0026#x3088;\u0026#x3073;\u0026#x4FE1;\u0026#x983C;\u0026#x533A;\u0026#x9593;\u0026#x306E;\u0026#x4E0A;\u0026#x9650;\u0026#x3068;\u0026#x4E0B;\u0026#x9650;\u0026#x3092;\u0026#x6700;\u0026#x5C24;\u0026#x63A8;\u0026#x5B9A;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x304C; p \u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3068;\u0026#x304D;\u0026#x306B; Cq \u0026#x5024;\u0026#x306E;\u0026#x89B3;\u0026#x6E2C;\u0026#x7D50;\u0026#x679C;\u0026#x3092;\u0026#x5F97;\u0026#x308B;\u0026#x78BA;\u0026#x7387;\u0026#x304C;\u0026#x3001;\u0026#x751F;\u0026#x6210;\u0026#x30E2;\u0026#x30C7;\u0026#x30EB;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x66F8;\u0026#x304D;\u0026#x4E0B;\u0026#x3055;\u0026#x308C;\u0026#x3001;\u0026#x3053;\u0026#x306E;\u0026#x3068;\u0026#x304D;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3054;\u0026#x3068;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x3067;\u0026#x3042;\u0026#x308D;\u0026#x3046;\u0026#x967D;\u0026#x6027;\u0026#x500B;\u0026#x4F53;\u0026#x306E;\u0026#x6570;\u0026#xFF08;m\u0026#xFF09;\u0026#x306F;\u0026#x3001;\u0026#x76F4;\u0026#x63A5;\u0026#x89B3;\u0026#x6E2C;\u0026#x3067;\u0026#x304D;\u0026#x306A;\u0026#x3044;\u0026#x6F5C;\u0026#x5728;\u0026#x5909;\u0026#x6570;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x6271;\u0026#x308F;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003ch3 class=\"mume-header\" id=\"%E5%80%8B%E4%BD%93%E3%81%82%E3%81%9F%E3%82%8A-dna-%E5%8F%8E%E9%87%8F%E3%81%AE%E3%81%B0%E3%82%89%E3%81%A4%E3%81%8D%E3%82%AC%E3%83%B3%E3%83%9E%E5%88%86%E5%B8%83%E3%81%8A%E3%82%88%E3%81%B3%E3%83%99%E3%83%BC%E3%82%BF%E5%88%86%E5%B8%83%E3%81%AB%E3%82%88%E3%82%8B%E8%BF%91%E4%BC%BC\"\u003e\u0026#x500B;\u0026#x4F53;\u0026#x3042;\u0026#x305F;\u0026#x308A; DNA \u0026#x53CE;\u0026#x91CF;\u0026#x306E;\u0026#x3070;\u0026#x3089;\u0026#x3064;\u0026#x304D;\u0026#xFF1A;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x304A;\u0026#x3088;\u0026#x3073;\u0026#x30D9;\u0026#x30FC;\u0026#x30BF;\u0026#x5206;\u0026#x5E03;\u0026#x306B;\u0026#x3088;\u0026#x308B;\u0026#x8FD1;\u0026#x4F3C;\u003c/h3\u003e\n\n\u003cp\u003e\u0026#x4F53;\u0026#x30B5;\u0026#x30A4;\u0026#x30BA;\u0026#x306E;\u0026#x5909;\u0026#x7570;\u0026#x3084;\u0026#xFF08;\u0026#x30C8;\u0026#x30E9;\u0026#x30C3;\u0026#x30D7;\u0026#x4E0A;\u0026#x3067;\u0026#x306E;\u0026#xFF09;\u0026#x6B7B;\u0026#x5F8C;\u0026#x306E; DNA \u0026#x5206;\u0026#x89E3;\u0026#x3068;\u0026#x3044;\u0026#x3063;\u0026#x305F;\u0026#x8981;\u0026#x56E0;\u0026#x306B;\u0026#x3088;\u0026#x308A;\u0026#x3001;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x500B;\u0026#x4F53;\u0026#x3042;\u0026#x305F;\u0026#x308A;\u0026#x306E; DNA \u0026#x53CE;\u0026#x91CF;\u0026#x306F;\u0026#x5927;\u0026#x304D;\u0026#x304F;\u0026#x3070;\u0026#x3089;\u0026#x3064;\u0026#x304F;\u0026#x3053;\u0026#x3068;\u0026#x304C;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003ccode\u003efreqpcr\u003c/code\u003e \u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x967D;\u0026#x6027;\u0026#x500B;\u0026#x4F53;\u0026#x6570;\u0026#x306E;\u0026#x4E0D;\u0026#x78BA;\u0026#x5B9F;\u0026#x6027;\u0026#xFF08;\u0026#x4E8C;\u0026#x9805;\u0026#x5206;\u0026#x5E03;\u0026#x306B;\u0026#x5F93;\u0026#x3046;\u0026#xFF09;\u0026#x306B;\u0026#x52A0;\u0026#x3048;\u0026#x3001;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3054;\u0026#x3068;\u0026#x306E; DNA \u0026#x53CE;\u0026#x91CF;\u0026#xFF08;Figure 1 \u0026#x304A;\u0026#x3088;\u0026#x3073; 2\u0026#xFF1A;\u0026#x5909;\u0026#x6570; X_R, X_S\u0026#xFF09;\u0026#x306E;\u0026#x4E0D;\u0026#x78BA;\u0026#x5B9F;\u0026#x6027;\u0026#x3092;\u0026#x3001;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x78BA;\u0026#x7387;\u0026#x5206;\u0026#x5E03;\u0026#x3067;\u0026#x8FD1;\u0026#x4F3C;\u0026#x3059;\u0026#x308B;\u0026#x624B;\u0026#x6CD5;\u0026#x3092;\u0026#x63A1;\u0026#x7528;\u0026#x3057;\u0026#x305F;\u0026#x3002;\u0026#x3053;\u0026#x306E;\u0026#x30A2;\u0026#x30A4;\u0026#x30C7;\u0026#x30A2;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x306F; \u003ca href=\"https://www.jstage.jst.go.jp/article/jpestics/46/2/46_D20-064/_article/-char/en\"\u003eSudo et al. (2021) Journal of Pesticide Science\u003c/a\u003e \u0026#x306B;\u0026#x8A73;\u0026#x3057;\u0026#x3044;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x306F;\u0026#x5F62;\u0026#x72B6;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x306E;\u0026#x8A2D;\u0026#x5B9A;\u0026#x6B21;\u0026#x7B2C;\u0026#x3067;\u0026#x3001;\u0026#x751F;\u0026#x7269;\u0026#x907A;\u0026#x4F53;\u0026#x7B49;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066; DNA \u0026#x304C;\u0026#x30E9;\u0026#x30F3;\u0026#x30C0;\u0026#x30E0;\u0026#x306B;\u0026#x5206;\u0026#x89E3;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x308A;\u0026#x3001;\u0026#x500B;\u0026#x4F53;\u0026#x3042;\u0026#x305F;\u0026#x308A;\u0026#x53CE;\u0026#x91CF;\u0026#x304C;\u0026#x5927;\u0026#x304D;\u0026#x304F;\u0026#x3070;\u0026#x3089;\u0026#x3064;\u0026#x304F;\u0026#x5834;\u0026#x5408;\u0026#xFF08;\u0026#x6307;\u0026#x6570;\u0026#x5206;\u0026#x5E03;\u0026#xFF09;\u0026#x304B;\u0026#x3089;\u0026#x3001;\u0026#x751F;\u0026#x4F53;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066; DNA \u0026#x53CE;\u0026#x91CF;\u0026#x304C;\u0026#x5E73;\u0026#x5747;\u0026#x5024;\u0026#x306E;\u0026#x5468;\u0026#x308A;\u0026#x306B;\u0026#x307B;\u0026#x307C;\u0026#x6B63;\u0026#x898F;\u0026#x5206;\u0026#x5E03;\u0026#x3059;\u0026#x308B;\u0026#x5834;\u0026#x5408;\u0026#x307E;\u0026#x3067;\u0026#x3092;\u0026#x3001;\u0026#x7D71;\u0026#x4E00;\u0026#x7684;\u0026#x306B;\u0026#x30E2;\u0026#x30C7;\u0026#x30EB;\u0026#x3067;\u0026#x8868;\u0026#x73FE;\u0026#x3067;\u0026#x304D;\u0026#x308B;\u0026#x3002;\u0026#x672C;\u0026#x30D1;\u0026#x30C3;\u0026#x30B1;\u0026#x30FC;\u0026#x30B8;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x308C;\u0026#x3070;\u0026#x3001;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x306E;\u0026#x5F62;\u0026#x72B6;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#xFF08;k\u0026#xFF09;\u0026#x3082;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6; p \u0026#x3068;\u0026#x540C;\u0026#x6642;\u0026#x306B;\u0026#x63A8;\u0026#x5B9A;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x30E6;\u0026#x30FC;\u0026#x30B6;\u0026#x30FC;\u0026#x306F;\u0026#x500B;\u0026#x4F53;\u0026#x3054;\u0026#x3068;\u0026#x306E; DNA \u0026#x91CF;\u0026#x306E;\u0026#x3070;\u0026#x3089;\u0026#x3064;\u0026#x304D;\u0026#x5EA6;\u0026#x5408;\u0026#x3044;\u0026#x3092;\u0026#x77E5;\u0026#x3089;\u0026#x306A;\u0026#x304F;\u0026#x3066;\u0026#x3082;\u0026#x3001;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x9811;\u0026#x5065;\u0026#x306A;\u0026#x63A8;\u0026#x5B9A;\u0026#x5024;\u0026#x3092;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x304C;\u0026#x6301;\u0026#x3064;\u0026#x597D;\u0026#x307E;\u0026#x3057;\u0026#x3044;\u0026#x6027;\u0026#x8CEA;\u0026#x306E; 1 \u0026#x3064;\u0026#x304C;\u0026#x300C;\u0026#x518D;\u0026#x751F;\u0026#x6027;\u0026#x300D;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x3042;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306B;\u0026#x7740;\u0026#x76EE;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x5404;\u0026#x500B;\u0026#x4F53;\u0026#x304C;\u0026#x6301;\u0026#x3064; DNA \u0026#x91CF;\u0026#x304C;\u0026#x72EC;\u0026#x7ACB;\u0026#x306B;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#xFF08;\u0026#x7B49;\u0026#x3057;\u0026#x3044;\u0026#x5C3A;\u0026#x5EA6;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x3092;\u0026#x6301;\u0026#x3064;\u0026#xFF09;\u0026#x306B;\u0026#x5F93;\u0026#x3046;\u0026#x306A;\u0026#x3089;\u0026#x3070;\u0026#x3001;\u0026#x8907;\u0026#x6570;\u0026#x500B;\u0026#x4F53;\u0026#x307E;\u0026#x3068;\u0026#x3081;\u0026#x305F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x5F53;\u0026#x8A72;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E; DNA \u0026#x91CF;\u0026#x3082;\u0026#x307E;\u0026#x305F;\u0026#x3001;\u0026#x5358;\u0026#x4E00;\u0026#x306E;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x306B;\u0026#x5F93;\u0026#x3046;\u0026#xFF08;Figure 2 \u0026#x5DE6;\u0026#xFF09;\u0026#x3002;\u003c/p\u003e\n\u003cp align=\"center\"\u003e\n\u003cimg src=\"./vignettes/figures/freqpcr_vignette_fig02.png\" style=\"width: 50%; height: 50%\" alt=\"Figure 2. \u0026#x500B;\u0026#x4F53; DNA \u0026#x53CE;\u0026#x91CF;\u0026#x306E;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x8FD1;\u0026#x4F3C;\u0026#x3068;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x306E;\u0026#x30D9;\u0026#x30FC;\u0026#x30BF;\u0026#x5206;\u0026#x5E03;\u0026#x306B;\u0026#x3088;\u0026#x308B;\u0026#x8868;\u0026#x73FE;\"\u003e\n\u003c/p\u003e\n\u003cblockquote\u003e\n\u003cp\u003eFigure 2.\u0026#xFF08;\u0026#x5DE6;\u0026#xFF09;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B; R \u0026#x306A;\u0026#x3044;\u0026#x3057; S \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x91CF;\u0026#x306F;\u0026#x3001;1 \u0026#x500B;\u0026#x4F53; 1 \u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x3042;\u0026#x305F;\u0026#x308A;\u0026#x306E; DNA \u0026#x53CE;\u0026#x91CF;\u0026#x3092;\u0026#x3001;\u0026#x5404;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x6301;\u0026#x3064;\u0026#x500B;\u0026#x4F53;\u0026#x6570;\u0026#x5206;\u0026#x3060;\u0026#x3051;\u0026#x8DB3;\u0026#x3057;\u0026#x5408;\u0026#x308F;\u0026#x305B;\u0026#x305F;\u0026#x91CF;\u0026#x306B;\u0026#x306A;\u0026#x308B;\u0026#x3002;\u0026#x56F3;\u0026#x306F;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x751F;\u0026#x7269; 6 \u0026#x500B;\u0026#x4F53;\u0026#x304B;\u0026#x3089; DNA \u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x305F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x3001;2 \u0026#x500B;\u0026#x4F53;\u0026#x304C; R \u0026#x3068;\u0026#x4EEE;\u0026#x5B9A;\u0026#x3057;\u0026#x305F;\u0026#x5834;\u0026#x5408;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x500B;\u0026#x4F53;\u0026#x3042;\u0026#x305F;\u0026#x308A;\u0026#x306E; DNA \u0026#x53CE;\u0026#x91CF;\u0026#x304C;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03; Ga(k = 1, \u0026#x3B8; = 10^(-6)) \u0026#x306B;\u0026#x5F93;\u0026#x3046;\u0026#x78BA;\u0026#x7387;\u0026#x5909;\u0026#x6570;\u0026#x306A;\u0026#x3089;\u0026#x3070;\u0026#x3001;2 \u0026#x500B;\u0026#x4F53;\u0026#x5206;\u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B; R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x91CF;\u0026#x306F; Ga(k = 2, \u0026#x3B8; = 10^(-6)) \u0026#x3067;\u0026#x8868;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x4E00;\u0026#x65B9;\u0026#x3001;4 \u0026#x500B;\u0026#x4F53;\u0026#x5206;\u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B; S \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x91CF;\u0026#x306F; Ga(k = 4, \u0026#x3B8; = 10^(-6)) \u0026#x306B;\u0026#x5F93;\u0026#x3046;\u0026#x3002;\u0026#xFF08;\u0026#x53F3;\u0026#xFF09;\u0026#x3053;\u0026#x306E;\u0026#x3068;\u0026#x304D;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x5360;\u0026#x3081;\u0026#x308B; R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x5272;\u0026#x5408;\u0026#x3001;\u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061; Y_R = X_R / (X_R + X_S) \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#x306F;\u0026#x3001;\u0026#x30D9;\u0026#x30FC;\u0026#x30BF;\u0026#x5206;\u0026#x5E03; Beta(shape1 = 2, shape2 = 4) \u0026#x306B;\u0026#x5F93;\u0026#x3046;\u0026#x3002;\u003c/p\u003e\n\u003c/blockquote\u003e\n\u003cp\u003e\u0026#x3055;\u0026#x3089;\u0026#x306B;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x306E;\u0026#x6027;\u0026#x8CEA;\u0026#x304B;\u0026#x3089;\u0026#x3001;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x4E2D;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B; 2 \u0026#x7A2E;\u0026#x985E;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x91CF;\u0026#x306E;\u0026#x6BD4;\u0026#x306F;\u0026#x3001;\u0026#x30D9;\u0026#x30FC;\u0026#x30BF;\u0026#x5206;\u0026#x5E03;\u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x78BA;\u0026#x7387;\u0026#x5206;\u0026#x5E03;\u0026#x306B;\u0026#x5F93;\u0026#x3046;\u0026#xFF08;Figure 2 \u0026#x53F3;\u0026#xFF09;\u0026#x3002;\u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x306E;\u0026#x6A19;\u0026#x6E96;\u0026#x8A2D;\u0026#x5B9A;\u0026#x3067;\u0026#x306F; 2 \u0026#x3064;\u0026#x306E;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x306E;\u0026#x4EE3;\u0026#x308F;\u0026#x308A;\u0026#x306B;\u0026#x3001;1 \u0026#x3064;\u0026#x306E;\u0026#x30D9;\u0026#x30FC;\u0026#x30BF;\u0026#x5206;\u0026#x5E03;\u0026#x3092;\u0026#x5229;\u0026#x7528;\u0026#x3057;\u0026#x3066;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x3092;\u0026#x8868;\u0026#x73FE;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x308C;\u0026#x306F;\u0026#x63A8;\u0026#x5B9A;\u0026#x306E;\u0026#x9AD8;\u0026#x901F;\u0026#x5316;\u0026#xFF08;\u0026#x6570;\u0026#x79D2;\u0026#x4EE5;\u0026#x5185;\u0026#x306B;\u0026#x8A08;\u0026#x7B97;\u0026#x304C;\u0026#x5B8C;\u0026#x4E86;\u0026#x3059;\u0026#x308B;\u0026#xFF09;\u0026#x306B;\u0026#x5BC4;\u0026#x4E0E;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x306A;\u0026#x304A; Figure 2 \u0026#x306B;\u0026#x793A;\u0026#x3057;\u0026#x305F; DNA \u0026#x53CE;\u0026#x91CF;\u0026#xFF08;X_R, X_S\u0026#xFF09;\u0026#x3084;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#xFF08;Y_R\u0026#xFF09;\u0026#x306F;\u0026#x3001;\u0026#x5404;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x578B;\u0026#x306B;\u0026#x8A72;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x500B;\u0026#x4F53;\u0026#x6570; m \u0026#x304C;\u0026#x56FA;\u0026#x5B9A;\u0026#x3055;\u0026#x308C;\u0026#x305F;\u0026#x3068;\u0026#x304D;\u0026#x306E;\u0026#x5024;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x306B;\u0026#x6CE8;\u0026#x610F;\u0026#x3092;\u0026#x8981;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x5B9F;\u0026#x969B;\u0026#x306B;\u0026#x306F; m \u0026#x3082;\u0026#x78BA;\u0026#x7387;\u0026#x5909;\u0026#x6570;\u0026#x306A;\u0026#x306E;\u0026#x3067; m = (1, 2, \u0026#x2026;, n-1) \u0026#x306E;\u0026#x305D;\u0026#x308C;\u0026#x305E;\u0026#x308C;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x3001;Beta(m, n - m) \u0026#x306E;\u0026#x4E0B;\u0026#x3067;\u0026#x5B9F;\u0026#x969B;\u0026#x306E; Cq \u0026#x5024;\u0026#x304C;\u0026#x89B3;\u0026#x6E2C;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x78BA;\u0026#x7387;\u0026#x3092;\u0026#x6C42;\u0026#x3081;\u0026#x3001;\u0026#x5C24;\u0026#x5EA6;\u0026#x95A2;\u0026#x6570;\u0026#x3092;\u0026#x5B9A;\u0026#x7FA9;\u0026#x3059;\u0026#x308B;\u0026#x306E;\u0026#x304C; \u003ccode\u003efreqpcr\u003c/code\u003e \u0026#x306E;\u0026#x4ED5;\u0026#x69D8;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x4EE5;\u0026#x4E0B;\u0026#x3001;Cq \u0026#x5024;\u0026#x306E;\u0026#x7D44;\u0026#x304C;\u0026#x3069;\u0026#x306E;\u0026#x3088;\u0026#x3046;\u0026#x306A;\u0026#x5B9F;\u0026#x9A13;\u0026#x30C7;\u0026#x30B6;\u0026#x30A4;\u0026#x30F3;\u0026#x3067;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x304B;\u0026#x3092;\u0026#x8FF0;\u0026#x3079;\u0026#x3066;\u0026#x3086;\u0026#x304F;\u0026#x3002;\u003c/p\u003e\n\u003chr\u003e\n\u003ch2 class=\"mume-header\" id=\"%E5%AE%9A%E9%87%8F-pcr-%E5%88%86%E6%9E%90%E3%82%92%E7%94%A8%E3%81%84%E3%81%9F%E3%82%A2%E3%83%AA%E3%83%AB%E9%A0%BB%E5%BA%A6%E6%8E%A8%E5%AE%9A%E3%81%AE%E5%8E%9F%E7%90%86%CE%B4%CE%B4cq-%E6%B3%95%E3%81%A8-red-%CE%B4%CE%B4cq-%E6%B3%95\"\u003e\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x63A8;\u0026#x5B9A;\u0026#x306E;\u0026#x539F;\u0026#x7406;\u0026#xFF1A;\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3068; RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u003c/h2\u003e\n\n\u003cp\u003e1 \u0026#x3064; 1 \u0026#x3064;\u0026#x306E;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B; 2 \u0026#x7A2E;\u0026#x985E;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x3001;\u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#xFF08;sample allele frequency\u0026#xFF09;\u0026#x3092;\u0026#x6E2C;\u0026#x5B9A;\u0026#x3059;\u0026#x308B;\u0026#x5B9F;\u0026#x9A13;\u0026#x624B;\u0026#x6CD5;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x73FE;\u0026#x5728;\u0026#x306E; \u003ccode\u003efreqpcr\u003c/code\u003e \u0026#x30D1;\u0026#x30C3;\u0026#x30B1;\u0026#x30FC;\u0026#x30B8;\u0026#x3067;\u0026#x306F;\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003eRED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#xFF1A;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x306B;\u0026#x3088;\u0026#x308B;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x524D;\u0026#x51E6;\u0026#x7406;\u0026#x3092;\u0026#x4F34;\u0026#x3046;\u0026#x5B9A;\u0026#x91CF; PCR\u0026#xFF08;Osakabe et al. 2017\u0026#xFF09;\u003c/li\u003e\n\u003cli\u003e\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x306A;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x306B;\u0026#x3088;\u0026#x308B; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#xFF08;Maeoka et al. 2020\u0026#xFF09;\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u0026#x306E; 2 \u0026#x7A2E;\u0026#x985E;\u0026#x306E;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x624B;\u0026#x6CD5;\u0026#xFF08;Figures 3, 4\u0026#xFF09;\u0026#x304C;\u0026#x516C;\u0026#x5F0F;\u0026#x306B;\u0026#x30B5;\u0026#x30DD;\u0026#x30FC;\u0026#x30C8;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u0026#x305D;\u0026#x306E;\u0026#x4ED6;\u0026#x306E; \u0026#x394;\u0026#x394;Cq \u0026#x3084; \u0026#x394;Cq \u0026#x306B;\u0026#x6E96;\u0026#x62E0;\u0026#x3057;\u0026#x305F;\u0026#x5206;\u0026#x6790;\u0026#x30D7;\u0026#x30ED;\u0026#x30C8;\u0026#x30B3;\u0026#x30EB;\u0026#x3067;\u0026#x3082;\u0026#x9069;\u0026#x7528;\u0026#x53EF;\u0026#x80FD;\u0026#x306A;\u0026#x3082;\u0026#x306E;\u0026#x306F;\u0026#x591A;\u0026#x3044;\u0026#x3068;\u0026#x601D;\u0026#x308F;\u0026#x308C;\u0026#x308B;\u0026#x306E;\u0026#x3067;\u0026#x3001;\u0026#x5171;\u0026#x540C;\u0026#x7814;\u0026#x7A76;\u0026#x306E;\u0026#x30AA;\u0026#x30D5;\u0026#x30A1;\u0026#x30FC;\u0026#x304C;\u0026#x3042;\u0026#x308C;\u0026#x3070;\u0026#x6B53;\u0026#x8FCE;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003ch3 class=\"mume-header\" id=\"red-%CE%B4%CE%B4cq-%E6%B3%95\"\u003eRED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u003c/h3\u003e\n\n\u003cp\u003eRED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#xFF08;\u0026#x394;\u0026#x394;Cq Method with Restriction Enzyme Digestion\u0026#xFF09;\u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x672A;\u0026#x77E5;\u0026#x983B;\u0026#x5EA6;\u0026#x3067;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x304C;\u0026#x6DF7;\u0026#x5408;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B; DNA \u0026#x6EB6;\u0026#x6DB2;\u0026#x304B;\u0026#x3089;\u0026#x3001;4 \u0026#x672C;\u0026#x306E;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3092;\u0026#x5206;\u0026#x6CE8;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x7570;\u0026#x306A;\u0026#x308B;\u0026#x51E6;\u0026#x7406;\u0026#x6761;\u0026#x4EF6;\u0026#x30FB;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x3067;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x3092;\u0026#x884C;\u0026#x3044;\u0026#x3001;\u0026#x394;\u0026#x394;Cq \u0026#x3092;\u0026#x8A08;\u0026#x7B97;\u0026#x3057;\u0026#x3066;\u0026#x5F53;\u0026#x8A72;\u0026#x6EB6;\u0026#x6DB2;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x6DF7;\u0026#x5408;\u0026#x6BD4;\u0026#x3092;\u0026#x6C42;\u0026#x3081;\u0026#x308B;\u0026#xFF08;Figure 3\u0026#xFF09;\u0026#x3002;Cq \u0026#x5024;\u0026#x3084; \u0026#x394;\u0026#x394;Cq \u0026#x5024;\u0026#x306E;\u0026#x5B9A;\u0026#x7FA9;\u0026#x81EA;\u0026#x4F53;\u0026#x306F;\u0026#x3001;\u0026#x4E00;\u0026#x822C;\u0026#x7684;\u0026#x306A;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x306E;\u0026#x305D;\u0026#x308C;\u0026#x3089;\u0026#xFF08;e.g. Livak \u0026amp; Schmittgen 2001; Vandesompele et al. 2002\u0026#xFF09;\u0026#x3068;\u0026#x540C;\u0026#x69D8;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003eRED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306E; PCR \u0026#x3067;\u0026#x306F;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x3092;\u0026#x3001;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3068;\u0026#x306F;\u0026#x7121;\u0026#x95A2;\u0026#x4FC2;\u0026#x306B;\u0026#x5897;\u0026#x5E45;\u0026#x3059;\u0026#x308B;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x304C;\u0026#x7528;\u0026#x3044;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x3088;\u0026#x3063;\u0026#x3066; DNA \u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x3066;\u0026#x5F97;\u0026#x305F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x6EB6;\u0026#x6DB2;\u0026#x3092;\u0026#x305D;\u0026#x306E;\u0026#x307E;\u0026#x307E; PCR \u0026#x306B;\u0026#x639B;\u0026#x3051;\u0026#x308B;\u0026#x306A;\u0026#x3089;\u0026#x3070;\u0026#x3001;(X_R + X_S) \u0026#x306E;\u0026#x548C;\u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#x306E;\u0026#x92F3;\u0026#x578B; DNA \u0026#x304C;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x308C;\u0026#x304C; control sample \u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x4E00;\u0026#x65B9;\u0026#x3001;\u0026#x5206;\u0026#x6CE8;\u0026#x3057;\u0026#x305F;\u0026#x6EB6;\u0026#x6DB2;\u0026#x306E;\u0026#x534A;\u0026#x5206;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x306F; PCR \u0026#x306E;\u0026#x524D;\u0026#x306B;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x3067;\u0026#x51E6;\u0026#x7406;\u0026#x3057;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x7247;\u0026#x65B9;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3060;\u0026#x3051;\u0026#x3092;\u0026#x6D88;\u0026#x53BB;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x3002;\u0026#x3053;\u0026#x3061;\u0026#x3089;\u0026#x304C; digested test sample \u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;S \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x3054;\u0026#x304F;\u0026#x4E00;\u0026#x90E8;\u0026#xFF08;z\u0026#xFF09;\u0026#x3060;\u0026#x3051;\u0026#x304C;\u0026#x6B8B;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x306B;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x3067;\u0026#x5207;\u0026#x65AD;\u0026#x3059;\u0026#x308B;\u0026#x3068;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x306E;\u0026#x92F3;\u0026#x578B;\u0026#x91CF;\u0026#x306F; (X_R + zX_S) \u0026#x76F8;\u0026#x5F53;\u0026#x3068;\u0026#x306A;\u0026#x308B;\u0026#x3002;\u0026#x5F93;\u0026#x3063;\u0026#x3066; digested test sample \u0026#x3068; control sample \u0026#x306E;\u0026#x92F3;\u0026#x578B;\u0026#x91CF;\u0026#x306E;\u0026#x5546;\u0026#x3067;\u0026#x3042;\u0026#x308B; (X_R + zX_S) / (X_R + X_S) \u0026#x304C;\u0026#x3001;z \u0026#x304C;\u0026#x5C0F;\u0026#x3055;\u0026#x3051;\u0026#x308C;\u0026#x3070; R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x304A;\u0026#x3088;\u0026#x305D;\u0026#x306E;\u0026#x5B58;\u0026#x5728;\u0026#x983B;\u0026#x5EA6;\u0026#x3068;\u0026#x306A;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u0026#x6CE8;\u0026#xFF1A;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x3067;\u0026#x5207;\u0026#x65AD;\u0026#x3059;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306F; R \u0026#x3067;\u0026#x3082; S \u0026#x3067;\u0026#x3082;\u0026#x3088;\u0026#x3044;\u0026#x3002;\u0026#x305F;\u0026#x3060;\u0026#x3057;\u0026#x63A8;\u0026#x5B9A;\u0026#x7CBE;\u0026#x5EA6;\u0026#x3092;\u0026#x4E0A;\u0026#x3052;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x306B;\u0026#x306F;\u0026#x3001;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x7A00;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3068;\u0026#x8003;\u0026#x3048;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x6B8B;\u0026#x3059;\u0026#xFF08;\u0026#x591A;\u0026#x3044;\u0026#x65B9;\u0026#x3092;\u0026#x5206;\u0026#x89E3;\u0026#x3059;\u0026#x308B;\u0026#xFF09;\u0026#x3068;\u0026#x3088;\u0026#x3044;\u0026#x3002;\u0026#x305F;\u0026#x3068;\u0026#x3048;\u0026#x3070;\u0026#x5730;\u0026#x57DF;\u0026#x306B;\u0026#x7A00;\u0026#x306B;\u0026#x5B58;\u0026#x5728;\u0026#x3059;\u0026#x308B;\u0026#x62B5;\u0026#x6297;\u0026#x6027;\u0026#x3092;\u0026#x691C;\u0026#x51FA;\u0026#x3057;\u0026#x305F;\u0026#x3051;\u0026#x308C;\u0026#x3070;\u0026#x3001;\u0026#x611F;\u0026#x53D7;\u0026#x6027;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3092;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x305D;\u0026#x306E;\u0026#x307B;\u0026#x3046;\u0026#x304C;\u0026#x5B9F;\u0026#x9A13;\u0026#x51E6;\u0026#x7406;\u0026#x9593;\u0026#x3067; Cq \u0026#x5024;\u0026#x306B;\u0026#x5927;\u0026#x304D;\u0026#x306A;\u0026#x5DEE;\u0026#x304C;\u0026#x73FE;\u0026#x308C;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x306B;\u0026#x306A;\u0026#x308A;\u0026#x3001;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x306E;\u0026#x6E2C;\u0026#x5B9A;\u0026#x8AA4;\u0026#x5DEE;\u0026#x306B;\u0026#x30DE;\u0026#x30B9;\u0026#x30AF;\u0026#x3055;\u0026#x308C;\u0026#x306B;\u0026#x304F;\u0026#x304F;\u0026#x306A;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003c/blockquote\u003e\n\u003cp\u003e\u0026#x305F;\u0026#x3068;\u0026#x3048;\u0026#x3070; Osakabe et al. (2017) \u0026#x306F;\u0026#x3001;\u0026#x30CA;\u0026#x30DF;\u0026#x30CF;\u0026#x30C0;\u0026#x30CB;\u0026#x306E;\u0026#x6BBA;\u0026#x30C0;\u0026#x30CB;\u0026#x5264;\u0026#xFF08;\u0026#x30A8;\u0026#x30C8;\u0026#x30AD;\u0026#x30B5;\u0026#x30BE;\u0026#x30FC;\u0026#x30EB;\u0026#xFF09;\u0026#x62B5;\u0026#x6297;\u0026#x6027;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3092;\u0026#x5B9A;\u0026#x91CF;\u0026#x3059;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x306B;\u0026#x3001;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x6EB6;\u0026#x6DB2;\u0026#x3092; 3 \u0026#x6642;\u0026#x9593;\u0026#x3001;Taq1 \u0026#x9175;\u0026#x7D20;\u0026#x3067;\u0026#x4E8B;\u0026#x524D;\u0026#x51E6;\u0026#x7406;\u0026#x3057;\u0026#x305F;\u0026#x3002;Taq1 \u0026#x306F; DNA \u0026#x4E0A;\u0026#x306E; TCGA \u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x5869;\u0026#x57FA;\u0026#x914D;\u0026#x5217;\u0026#x304C;\u0026#x3042;\u0026#x308B;\u0026#x7B87;\u0026#x6240;\u0026#x3092;\u0026#x8A8D;\u0026#x8B58;\u0026#x3057;\u0026#x3066;\u0026#x5207;\u0026#x65AD;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x30A8;\u0026#x30C8;\u0026#x30AD;\u0026#x30B5;\u0026#x30BE;\u0026#x30FC;\u0026#x30EB;\u0026#x62B5;\u0026#x6297;\u0026#x6027;\u0026#x306F;\u0026#x30AD;\u0026#x30C1;\u0026#x30F3;\u0026#x5408;\u0026#x6210;\u0026#x9175;\u0026#x7D20;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#xFF08;CHS1\u0026#xFF09;\u0026#x306E;\u0026#x5869;\u0026#x57FA;\u0026#x914D;\u0026#x5217; TCG \u003cem\u003e\u003cstrong\u003eA\u003c/strong\u003e\u003c/em\u003e TT \u0026#x304C; TCG \u003cem\u003e\u003cstrong\u003eT\u003c/strong\u003e\u003c/em\u003e TT \u0026#x306B;\u0026#x5909;\u0026#x7570;\u0026#x3057;\u0026#x3066;\u0026#x751F;\u0026#x3058;\u0026#x308B;\u0026#x306E;\u0026#x3067;\u0026#x3001;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x3059;\u0026#x308B;\u0026#x3068;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x611F;\u0026#x53D7;\u0026#x6027;\u0026#x500B;\u0026#x4F53;\u0026#x7531;\u0026#x6765;\u0026#x306E; DNA \u0026#x304C;\u0026#x5207;\u0026#x65AD;\u0026#x3055;\u0026#x308C;\u0026#x305F;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3092;\u0026#x5F97;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp align=\"center\"\u003e\n\u003cimg src=\"./vignettes/figures/freqpcr_vignette_fig03.png\" style=\"width: 50%; height: 50%\" alt=\"Figure 3. RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304F;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x4E2D;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x306E;\u0026#x6E2C;\u0026#x5B9A;\"\u003e\n\u003c/p\u003e\n\u003cblockquote\u003e\n\u003cp\u003eFigure 3. \u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x4E2D;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x306E;\u0026#x6E2C;\u0026#x5B9A; (1) RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3002;\u0026#x7570;\u0026#x306A;\u0026#x308B;\u0026#x4E8B;\u0026#x524D;\u0026#x51E6;\u0026#x7406;\u0026#xFF08;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#xFF09;\u0026#x3092;\u0026#x53D7;\u0026#x3051;\u0026#x305F;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x9593;\u0026#x3067;\u0026#x92F3;\u0026#x578B; DNA \u0026#x91CF;\u0026#x3092;\u0026#x6BD4;\u0026#x8F03;\u0026#x3059;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x306E;\u0026#x88DC;\u0026#x6B63;\u0026#x57FA;\u0026#x6E96;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x751F;\u0026#x7269;\u0026#x4F53;\u0026#x5185;\u0026#x306B;\u0026#x4E00;\u0026#x5B9A;\u0026#x91CF;\u0026#x304C;\u0026#x5B58;\u0026#x5728;\u0026#x3059;\u0026#x308B;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#xFF08;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#xFF09;\u0026#x3092;\u0026#x5BFE;\u0026#x8C61;\u0026#x3068;\u0026#x3057;\u0026#x305F; PCR \u0026#x5897;\u0026#x5E45;\u0026#x3092;\u0026#x4E26;\u0026#x884C;\u0026#x3057;\u0026#x3066;\u0026#x5B9F;\u0026#x65BD;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x306E;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x3082;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x3082;\u0026#x3001;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x306F;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x554F;\u0026#x308F;\u0026#x305A;\u0026#x306B;\u0026#x5897;\u0026#x5E45;\u0026#x3059;\u0026#x308B;\u0026#x3082;\u0026#x306E;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u0026#x305F;\u0026#x3060;\u0026#x3057;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x3092;\u0026#x53D7;\u0026#x3051;\u0026#x305F;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B; S \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x6301;\u0026#x3064; DNA \u0026#x5206;\u0026#x5B50;\u0026#x306E;\u0026#x307F;\u0026#x3001;\u0026#x30B5;\u0026#x30A4;\u0026#x30C8;\u0026#x304C;\u0026#x9014;\u0026#x4E2D;\u0026#x3067;\u0026#x5207;\u0026#x65AD;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x5897;\u0026#x5E45;\u0026#x5BFE;\u0026#x8C61;\u0026#x304B;\u0026#x3089;\u0026#x5916;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x306E;\u0026#x92F3;\u0026#x578B; DNA \u0026#x91CF;\u0026#x306E;\u0026#x9055;\u0026#x3044;\u0026#x304C; R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x983B;\u0026#x5EA6;\u0026#x3001;\u0026#x53B3;\u0026#x5BC6;\u0026#x306B;\u0026#x306F; (X_R + zX_S) / (X_R + X_S) = Y_R + z(1\u0026#x2212;Y_R) \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x6E2C;\u0026#x5B9A;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x3053;\u0026#x306E; (X_R + zX_S) / (X_R + X_S) \u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x5024;\u0026#x306F;\u0026#x56F3;\u0026#x4E2D;\u0026#x3067; (X^TD / X^HD) \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x8A08;\u0026#x7B97;\u0026#x3055;\u0026#x308C;\u0026#x308B; Cq \u0026#x5024;\u0026#x306E;\u0026#x5DEE;\u0026#x5206;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x304C;\u0026#x3001;\u0026#x4F59;\u0026#x8A08;\u0026#x306A;\u0026#x4FC2;\u0026#x6570; \u0026#x3B4;_T \u0026#x304C;\u0026#x5165;\u0026#x3063;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x308C;\u0026#x306F; (X^TD / X^HD) / (X^TW / X^HW) \u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x6307;\u0026#x6A19;\u0026#x3092;\u0026#x8A08;\u0026#x7B97;\u0026#x3059;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x3067;\u0026#x30AD;\u0026#x30E3;\u0026#x30F3;\u0026#x30BB;\u0026#x30EB;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x308C;\u0026#x3092;\u0026#x5BFE;\u0026#x6570;\u0026#x30B9;\u0026#x30B1;\u0026#x30FC;\u0026#x30EB;\u0026#xFF08;PCR \u0026#x306E;\u0026#x30B5;\u0026#x30A4;\u0026#x30AF;\u0026#x30EB;\u0026#x6570;\u0026#xFF09;\u0026#x3067;\u0026#x8868;\u0026#x73FE;\u0026#x3059;\u0026#x308B;\u0026#x3068; Cq \u0026#x5024;\u0026#x306E;\u0026#x300C;\u0026#x5DEE;\u0026#x5206;\u0026#x306E;\u0026#x5DEE;\u0026#x5206;\u0026#x300D;\u0026#x3068;\u0026#x306A;\u0026#x308A;\u0026#x3001;\u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061; \u0026#x394;\u0026#x394;Cq \u0026#x5024;\u0026#x306B;\u0026#x4ED6;\u0026#x306A;\u0026#x3089;\u0026#x306A;\u0026#x3044;\u0026#x3002;\u003c/p\u003e\n\u003c/blockquote\u003e\n\u003cp\u003e\u0026#x305F;\u0026#x3060;\u0026#x3057;\u0026#x3001;digested test sample \u0026#x3068; control sample \u0026#x3092;\u0026#x305D;\u0026#x308C;\u0026#x305E;\u0026#x308C;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x5897;\u0026#x5E45;\u0026#x3057;\u0026#x305F;\u0026#x7D50;\u0026#x679C;\u0026#x3092;\u0026#x3001;\u0026#x5358;\u0026#x7D14;\u0026#x306B;\u0026#x5272;\u0026#x308A;\u0026#x7B97;\u0026#x3059;\u0026#x308B;\u0026#x3060;\u0026#x3051;\u0026#xFF08;Figure 3 \u0026#x306E;\u0026#x92F3;\u0026#x578B;\u0026#x91CF;\u0026#x3067;\u0026#x3044;\u0026#x3046;\u0026#x3068; (X^TD / X^TW) \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#xFF09;\u0026#x3067;\u0026#x306F;\u0026#x4E0A;\u0026#x624B;\u0026#x304F;\u0026#x884C;\u0026#x304B;\u0026#x306A;\u0026#x3044;\u0026#x3002;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x306B;\u0026#x4F34;\u0026#x3063;\u0026#x3066;\u0026#x3001;\u0026#x6EB6;\u0026#x6DB2;\u0026#x306E;\u0026#x91CF;\u0026#x3084;\u0026#x6027;\u0026#x8CEA;\u0026#x304C;\u0026#x4E0D;\u0026#x968F;\u0026#x610F;\u0026#x306B;\u0026#x5909;\u0026#x5316;\u0026#x3057;\u0026#x3046;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x3060;\u0026#xFF08;Figure 3 \u0026#x3067;\u0026#x4FC2;\u0026#x6570; \u0026#x3B4;_B \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x8868;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x91CF;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#xFF09;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x305D;\u0026#x3053;\u0026#x3067;\u0026#x5185;\u0026#x90E8;\u0026#x6A19;\u0026#x6E96;\u0026#x3001;\u0026#x5178;\u0026#x578B;\u0026#x7684;\u0026#x306B;\u0026#x306F;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#xFF08;Osakabe et al. 2017 \u0026#x3067;\u0026#x306F; GAPDH\u0026#xFF09;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x5897;\u0026#x5E45;\u0026#x3057;\u0026#x305F;\u0026#x7D50;\u0026#x679C;\u0026#xFF08;X^HD \u0026#x3068; X^HW \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#xFF09;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x5148;\u0026#x306B;\u0026#x5404;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306E;\u0026#x92F3;\u0026#x578B;\u0026#x91CF;\u0026#x3092;\u0026#x88DC;\u0026#x6B63;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x3002;\u0026#x92F3;\u0026#x578B; DNA \u0026#x91CF;\u0026#x306E;\u0026#x6BD4;\u0026#x306F; Cq \u0026#x5024;\u0026#xFF08;\u0026#x30B5;\u0026#x30A4;\u0026#x30AF;\u0026#x30EB;\u0026#x6570;\u0026#x3002;\u0026#x5BFE;\u0026#x6570;\u0026#x30B9;\u0026#x30B1;\u0026#x30FC;\u0026#x30EB;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#xFF09;\u0026#x306E;\u0026#x5DEE;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x8868;\u0026#x73FE;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x306E;\u0026#x3067;\u0026#x3001;digested test sample \u0026#x3068; control sample \u0026#x306E;\u0026#x5404;\u0026#x3005;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x3001;(\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x306E; Cq \u0026#x5024;)\u0026#x2212;(\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x306E; Cq \u0026#x5024;) \u0026#x3092;\u0026#x8A08;\u0026#x7B97;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x3046;\u0026#x3057;\u0026#x305F; Cq \u0026#x5024;\u0026#x306E;\u0026#x5DEE;\u0026#x5206;\u0026#xFF08;digested test sample \u0026#x3067;\u0026#x306F; X^TD / X^HD\u0026#x3001;control sample \u0026#x3067;\u0026#x306F; X^TW / X^HW \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#xFF09;\u0026#x306E;\u0026#x3053;\u0026#x3068;\u0026#x3092; \u0026#x394;Cq \u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x540C;\u0026#x3058;\u0026#x51E6;\u0026#x7406;\u0026#x3092;\u0026#x53D7;\u0026#x3051;\u0026#x305F;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3067;\u0026#x3082;\u0026#x3001;\u0026#x751F;\u0026#x4F53;\u0026#x5185;\u0026#x3067;\u0026#x306E; DNA \u0026#x30B3;\u0026#x30D4;\u0026#x30FC;\u0026#x6570;\u0026#x306F;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x3088;\u0026#x3063;\u0026#x3066;\u0026#x7570;\u0026#x306A;\u0026#x308B;\u0026#x304B;\u0026#x3082;\u0026#x3057;\u0026#x308C;\u0026#x306A;\u0026#x3044;\u0026#x3002;\u0026#x305D;\u0026#x308C;\u0026#x305E;\u0026#x308C;\u0026#x6C42;\u0026#x3081;\u0026#x305F; \u0026#x394;Cq \u0026#x5024;\u0026#x306B;\u0026#x306F;\u0026#x3001;\u0026#x3053;\u0026#x306E;\u0026#x5DEE;\u0026#x304C;\u0026#x4FC2;\u0026#x6570; \u0026#x3B4;_T \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x6B8B;\u0026#x3063;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u0026#x4F59;\u0026#x5206;\u0026#x306A;\u0026#x4FC2;\u0026#x6570;\u0026#x3092;\u0026#x542B;\u0026#x307E;\u0026#x306A;\u0026#x3044; (X_R + zX_S) / (X_R + X_S) \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#x3092;\u0026#x5F97;\u0026#x308B;\u0026#x306B;\u0026#x306F;\u0026#x3001;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x3042;\u0026#x308A;\u0026#x30FB;\u0026#x306A;\u0026#x3057;\u0026#x306E;\u0026#x6761;\u0026#x4EF6;\u0026#x9593;\u0026#x3067; \u0026#x394;Cq \u0026#x5024;\u0026#x540C;\u0026#x58EB;\u0026#x306E;\u0026#x5DEE;\u0026#x5206;\u0026#x3092;\u0026#x53D6;\u0026#x308C;\u0026#x3070;\u0026#x3088;\u0026#x3044;\u0026#x3002;\u0026#x3053;\u0026#x3046;\u0026#x3057;\u0026#x3066;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x308B; \u0026#x394;\u0026#x394;Cq \u0026#x5024;\u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061; (X^TD / X^HD) / (X^TW / X^HW) \u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x3B4;_B \u0026#x3082; \u0026#x3B4;_T \u0026#x3082;\u0026#x30AD;\u0026#x30E3;\u0026#x30F3;\u0026#x30BB;\u0026#x30EB;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x3088;\u0026#x3063;\u0026#x3066; RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306F;\u0026#x691C;\u0026#x91CF;\u0026#x7DDA;\u0026#x3092;\u0026#x5FC5;\u0026#x8981;\u0026#x3068;\u0026#x305B;\u0026#x305A;\u0026#x3001;\u0026#x691C;\u0026#x67FB;\u0026#x5BFE;\u0026#x8C61;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x672A;\u0026#x77E5;\u0026#x6EB6;\u0026#x6DB2;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x3092;\u0026#x6E2C;\u0026#x5B9A;\u0026#x3067;\u0026#x304D;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x306A;\u0026#x304A; (X_R + zX_S) / (X_R + X_S) \u0026#x306F;\u0026#x3001;z \u0026#x304C;\u0026#x672A;\u0026#x77E5;\u0026#x306A;\u0026#x5834;\u0026#x5408;\u0026#x306F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x4E2D;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6; Y_R = X_R / (X_R + X_S) \u0026#x305D;\u0026#x306E;\u0026#x3082;\u0026#x306E;\u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x304F;\u0026#x3001;\u0026#x8FD1;\u0026#x4F3C;\u0026#x5024;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x305D;\u0026#x3053;\u0026#x3067; Osakabe et al. (2017) \u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x3092;\u0026#x9577;\u0026#x6642;\u0026#x9593;\u0026#x884C;\u0026#x3063;\u0026#x3066; z \u0026#x3092;\u0026#x6975;\u0026#x529B;\u0026#x5C0F;\u0026#x3055;\u0026#x304F;\u0026#x3059;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x3092;\u0026#x63A8;\u0026#x5968;\u0026#x3057;\u0026#x305F;\u0026#x3002;\u0026#x4E00;\u0026#x65B9; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x3066;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x3092;\u0026#x63A8;\u0026#x5B9A;\u0026#x3059;\u0026#x308B;\u0026#x5834;\u0026#x5408;\u0026#x3001;z \u0026#x306E;\u0026#x30B5;\u0026#x30A4;\u0026#x30BA;\u0026#x304C;\u0026#x591A;\u0026#x5C11;\u0026#x5927;\u0026#x304D;\u0026#x304F;\u0026#x3066;\u0026#x3082;\u0026#x65E2;\u0026#x77E5;\u0026#x3067;\u0026#x3055;\u0026#x3048;\u0026#x3042;\u0026#x308C;\u0026#x3070;\u0026#x63A8;\u0026#x5B9A;\u0026#x304C;\u0026#x53EF;\u0026#x80FD;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x3080;\u0026#x3057;\u0026#x308D; z \u0026#x304C;\u0026#x5C0F;\u0026#x3055;\u0026#x3059;\u0026#x304E;\u0026#x308B;\u0026#xFF08;10^{-5} \u0026#x4EE5;\u0026#x4E0B;\u0026#xFF09;\u0026#x3068;\u0026#x3001;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B; R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x304C;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x306A;\u0026#x3044;\u0026#x969B;\u0026#x306B;\u0026#x300C;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x3042;\u0026#x308A;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x300D;\u0026#x306E; qPCR \u0026#x306B;\u0026#x8981;\u0026#x3059;\u0026#x308B;\u0026#x30B5;\u0026#x30A4;\u0026#x30AF;\u0026#x30EB;\u0026#x304C;\u0026#x9577;\u0026#x3059;\u0026#x304E;\u0026#x3066;\u0026#x3001;\u0026#x53CD;\u0026#x5FDC;\u0026#x304C;\u0026#x7ACB;\u0026#x3061;\u0026#x4E0A;\u0026#x304C;\u0026#x3063;\u0026#x3066;\u0026#x3053;\u0026#x306A;\u0026#x3044;\u0026#x6050;\u0026#x308C;\u0026#x304C;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003ch3 class=\"mume-header\" id=\"%E3%82%A2%E3%83%AA%E3%83%AB%E7%89%B9%E7%95%B0%E7%9A%84%E3%83%97%E3%83%A9%E3%82%A4%E3%83%9E%E3%83%BC%E3%82%BB%E3%83%83%E3%83%88%E3%82%92%E7%94%A8%E3%81%84%E3%81%9F-%CE%B4%CE%B4cq-%E6%B3%95\"\u003e\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u003c/h3\u003e\n\n\u003cp\u003eOsakabe et al. (2017) \u0026#x306E; RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3092;\u0026#x9069;\u0026#x7528;\u0026#x3059;\u0026#x308B;\u0026#x306B;\u0026#x306F;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x7247;\u0026#x65B9;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306B;\u0026#x4F5C;\u0026#x7528;\u0026#x3059;\u0026#x308B;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x3092;\u0026#x898B;\u0026#x3064;\u0026#x3051;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x304C;\u0026#x8981;\u0026#x4EF6;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x306E;\u0026#x5236;\u0026#x7D04;\u0026#x3092;\u0026#x53D6;\u0026#x308A;\u0026#x9664;\u0026#x304F;\u0026#x305F;\u0026#x3081; Maeoka et al. (2020) \u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x306A;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306B;\u0026#x3088;\u0026#x308B;\u0026#x3001;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x6E2C;\u0026#x5B9A;\u0026#x624B;\u0026#x6CD5;\u0026#x304C;\u0026#x63D0;\u0026#x6848;\u0026#x3055;\u0026#x308C;\u0026#x305F;\u0026#xFF08;Figure 4\u0026#xFF09;\u0026#x3002;\u003c/p\u003e\n\u003cp align=\"center\"\u003e\n\u003cimg src=\"./vignettes/figures/freqpcr_vignette_fig04.png\" style=\"width: 50%; height: 50%\" alt=\"Figure 4. \u0026#x4E00;\u0026#x822C;\u0026#x7684;\u0026#x306A; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304F;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x4E2D;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x306E;\u0026#x6E2C;\u0026#x5B9A;\"\u003e\n\u003c/p\u003e\n\u003cblockquote\u003e\n\u003cp\u003eFigure 4. \u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x4E2D;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x306E;\u0026#x6E2C;\u0026#x5B9A;\u0026#xFF1A;(2) \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#xFF08;Maeoka et al. 2020\u0026#xFF09;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x3053;\u0026#x3061;\u0026#x3089;\u0026#x3082; (X^TD / X^HD) / (X^TW / X^HW) = Y_R + z(1 \u0026#x2212; Y_R) \u0026#x3067;\u0026#x62B5;\u0026#x6297;\u0026#x6027;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x3092;\u0026#x8FD1;\u0026#x4F3C;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x305F;\u0026#x3060;\u0026#x3057; RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3068;\u0026#x7570;\u0026#x306A;\u0026#x308A;\u0026#x3001;\u0026#x30C6;\u0026#x30B9;\u0026#x30C8;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3068; control sample \u0026#x3067;\u0026#x306F;\u0026#x7531;\u0026#x6765;\u0026#x304C;\u0026#x305D;\u0026#x3082;\u0026#x305D;\u0026#x3082;\u0026#x7570;\u0026#x306A;\u0026#x308B;\u0026#x305F;\u0026#x3081; \u0026#x3B4;_B \u0026#x306F;\u0026#x5B9A;\u0026#x7FA9;\u0026#x305B;\u0026#x305A;\u0026#x3001;\u0026#x4EE3;\u0026#x308F;\u0026#x308A;\u0026#x306B; control sample \u0026#x306E;\u0026#x92F3;\u0026#x578B; DNA \u0026#x91CF;\u0026#x3092; X\u0026#x2019;_R \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x8A18;\u0026#x53F7;\u0026#x81EA;\u0026#x4F53;\u0026#x3092;\u0026#x5909;\u0026#x3048;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003c/blockquote\u003e\n\u003cp\u003e\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x304C;\u0026#x4E0D;\u0026#x660E;\u0026#x306A; test sample\u0026#xFF08;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x306F;\u0026#x4E0D;\u0026#x8981;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#xFF09;\u0026#x306E; DNA \u0026#x6EB6;\u0026#x6DB2;\u0026#x304B;\u0026#x3089;\u0026#x3001;2 \u0026#x672C;\u0026#x307B;\u0026#x3069;\u0026#x5206;\u0026#x6CE8;\u0026#x3057;\u0026#x3066;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3068;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3067;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x5897;\u0026#x5E45;\u0026#x3057;\u0026#x3001;Cq \u0026#x5024;\u0026#x3092;\u0026#x6E2C;\u0026#x5B9A;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x305F;\u0026#x3060;\u0026#x3057;\u0026#x4ECA;\u0026#x5EA6;\u0026#x306F;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3092;\u0026#x5897;\u0026#x5E45;\u0026#x3059;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x306E;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x3092;\u0026#x3001;R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3060;\u0026#x3051;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x306B;\u0026#x5897;\u0026#x5E45;\u0026#x3059;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x306B;\u0026#x8A2D;\u0026#x8A08;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x3002;\u0026#x5F93;\u0026#x3063;\u0026#x3066;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3067;\u0026#x306F; X^HD = (X_R + X_S) \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#x304C;\u0026#x5897;\u0026#x5E45;\u0026#x5BFE;\u0026#x8C61;\u0026#x3060;\u0026#x304C;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3067;\u0026#x306F; X^TD = \u0026#x3B4;_T(X_R + zX_S) \u0026#x304C;\u0026#x5897;\u0026#x5E45;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x672C;\u0026#x6CD5;\u0026#x306B;\u0026#x304A;\u0026#x3051;\u0026#x308B; z \u0026#x306F;\u0026#x3001;\u0026#x50C5;\u0026#x304B;\u0026#x306B;\u0026#x751F;\u0026#x3058;\u0026#x308B;\u0026#x975E;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x306A;\u0026#x5897;\u0026#x5E45;\u0026#x306E;\u0026#x5BFE;\u0026#x8C61;\u0026#x3068;\u0026#x306A;\u0026#x308B; S \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x5272;\u0026#x5408;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e(X^TD / X^HD) \u0026#x306B;\u0026#x306F;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306E;\u0026#x9593;\u0026#x3067;\u0026#x306E;\u0026#x92F3;\u0026#x578B; DNA \u0026#x91CF;\u0026#x306E;\u0026#x6BD4;\u0026#x3001;\u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061; \u0026#x3B4;_T \u0026#x304C;\u0026#x672A;\u0026#x3060;\u0026#x4FC2;\u0026#x6570;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x639B;\u0026#x304B;\u0026#x3063;\u0026#x305F;\u0026#x307E;\u0026#x307E;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x3053;\u0026#x308C;\u0026#x3092; RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3068;\u0026#x540C;\u0026#x69D8;\u0026#x3001;control sample \u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x3066;\u0026#x30AD;\u0026#x30E3;\u0026#x30F3;\u0026#x30BB;\u0026#x30EB;\u0026#x3059;\u0026#x308B;\u0026#x3002;\n\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3067;\u0026#x306F;\u0026#x3001;RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3067;\u0026#x7528;\u0026#x3044;\u0026#x3089;\u0026#x308C;\u0026#x305F;\u0026#x30B3;\u0026#x30F3;\u0026#x30C8;\u0026#x30ED;\u0026#x30FC;\u0026#x30EB;\u0026#x3001;\u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x3092;\u0026#x4F34;\u0026#x308F;\u0026#x306A;\u0026#x3044;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x6EB6;\u0026#x6DB2;\u0026#x81EA;\u0026#x4F53;\u0026#x306E;\u0026#x4EE3;\u0026#x308F;\u0026#x308A;\u0026#x306B;\u0026#x3001;\u0026#x78BA;\u0026#x5B9F;\u0026#x306B; R \u0026#x3068;\u0026#x5206;\u0026#x304B;\u0026#x3063;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x500B;\u0026#x4F53;\u0026#x304B;\u0026#x3089;\u0026#x306E; DNA \u0026#x62BD;\u0026#x51FA;\u0026#x6DB2;\u0026#x3092; control sample \u0026#x306B;\u0026#x7528;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u0026#x5178;\u0026#x578B;\u0026#x7684;\u0026#x306B;\u0026#x306F;\u0026#x3001;\u0026#x5BA4;\u0026#x5185;\u0026#x98FC;\u0026#x80B2;\u0026#x3067;\u0026#x9078;\u0026#x629C;\u0026#x3057;\u0026#x305F;\u0026#x7D14;\u0026#x7CFB;\u0026#x306E; R \u0026#x500B;\u0026#x4F53;\u0026#x3092;\u0026#x4F7F;\u0026#x3046;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003eControl sample \u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3067;\u0026#x306F; X^HW = X\u0026#x2019;_R \u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3067;\u0026#x306F; X^TW = \u0026#x3B4;_T X\u0026#x2019;_R \u0026#x304C;\u0026#x5897;\u0026#x5E45;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#xFF08;\u0026#x53CD;\u0026#x5FDC;\u0026#x6DB2;\u0026#x306E;\u0026#x7531;\u0026#x6765;\u0026#x304C;\u0026#x7570;\u0026#x306A;\u0026#x308B;\u0026#x306E;\u0026#x3067; X \u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x304F; X\u0026#x2019; \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#xFF09;\u0026#x3002;\u0026#x3064;\u0026#x307E;\u0026#x308A; \u0026#x394;Cq \u0026#x5024;\u0026#x3092;\u0026#x8A08;\u0026#x7B97;\u0026#x3059;\u0026#x308C;\u0026#x3070;\u0026#x3001;\u0026#x305D;\u0026#x306E;\u0026#x307E;\u0026#x307E; \u0026#x3B4;_T \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#x304C;\u0026#x6C42;\u0026#x3081;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x6700;\u0026#x7D42;\u0026#x7684;\u0026#x306B; \u0026#x394;\u0026#x394;Cq \u0026#x306E;\u0026#x5F62;\u0026#x306B;\u0026#x6301;\u0026#x3061;\u0026#x8FBC;\u0026#x3080;\u0026#x3053;\u0026#x3068;\u0026#x3067;\u0026#x3001; (X^TD / X^HD) / (X^TW / X^HW) = (X_R + zX_S) / (X_R + X_S) \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#x3092;\u0026#x5F97;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003ch3 class=\"mume-header\" id=\"%CE%B4cq-%E6%B3%95%E3%81%AB%E3%82%88%E3%82%8B%E3%82%A2%E3%83%AA%E3%83%AB%E9%A0%BB%E5%BA%A6%E3%81%AE%E6%8E%A8%E5%AE%9A\"\u003e\u0026#x394;Cq \u0026#x6CD5;\u0026#x306B;\u0026#x3088;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x63A8;\u0026#x5B9A;\u003c/h3\u003e\n\n\u003cp\u003e\u0026#x306A;\u0026#x304A; \u0026#x3B4;_T \u0026#x306E;\u0026#x30B5;\u0026#x30A4;\u0026#x30BA;\u0026#x304C;\u0026#x65E2;\u0026#x77E5;\u0026#x3067;\u0026#x3042;\u0026#x308C;\u0026#x3070;\u0026#x3001;test sample \u0026#x3060;\u0026#x3051;\u0026#x3092;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3068;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3067;\u0026#x5897;\u0026#x5E45;\u0026#x3057;\u0026#x3066; X^TD \u0026#x3068; X^HD \u0026#x3092;\u0026#x5F97;\u0026#x3066;\u0026#x304B;\u0026#x3089;\u0026#x3001;\u0026#x3B4;_T \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x91CF;\u0026#x3092;\u0026#x5DEE;\u0026#x3057;\u0026#x5F15;\u0026#x304F;\u0026#x3053;\u0026#x3068;\u0026#x3067;\u0026#x3082;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x3092;\u0026#x63A8;\u0026#x5B9A;\u0026#x3067;\u0026#x304D;\u0026#x308B;\u0026#x3002;\u0026#x3064;\u0026#x307E;\u0026#x308A; \u0026#x394;Cq \u0026#x6CD5;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x30D0;\u0026#x30FC;\u0026#x30B8;\u0026#x30E7;\u0026#x30F3; 0.4.0 \u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x3001;X^TW \u0026#x3068; X^HW \u0026#x3092;\u0026#x6B20;\u0026#x3044;\u0026#x305F;\u0026#x30C7;\u0026#x30FC;\u0026#x30BF;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x3082;\u0026#x53D7;\u0026#x3051;\u0026#x5165;\u0026#x308C;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x4ED5;\u0026#x69D8;\u0026#x306B; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x304C;\u0026#x62E1;\u0026#x5F35;\u0026#x3055;\u0026#x308C;\u0026#x305F;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x3053;\u0026#x306E;\u0026#x5834;\u0026#x5408; \u0026#x3B4;_T\u0026#xFF08;\u0026#x5F15;\u0026#x6570; \u003ccode\u003etargetScale\u003c/code\u003e\u0026#xFF09;\u0026#x304A;\u0026#x3088;\u0026#x3073;\u0026#x3001;\u0026#x6E2C;\u0026#x5B9A;\u0026#x3055;\u0026#x308C;\u0026#x305F; Cq \u0026#x5024;\u0026#x304C;\u0026#x5F93;\u0026#x3046;\u0026#x6B63;\u0026#x898F;\u0026#x8AA4;\u0026#x5DEE;\u0026#x306E;\u0026#x6A19;\u0026#x6E96;\u0026#x504F;\u0026#x5DEE; \u0026#x3C3;_c\u0026#xFF08;\u003ccode\u003esdMeasure\u003c/code\u003e\u0026#xFF09;\u0026#x306F;\u0026#x3001;\u0026#x4E8B;\u0026#x524D;\u0026#x306B;\u0026#x65E2;\u0026#x77E5;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x6EB6;\u0026#x6DB2;\u0026#x3067;\u0026#x63A8;\u0026#x5B9A;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x3002;\u0026#x4E0B;\u0026#x306E;\u0026#x30BB;\u0026#x30AF;\u0026#x30B7;\u0026#x30E7;\u0026#x30F3;\u0026#x306B;\u0026#x3042;\u0026#x308B; \u003ccode\u003eknownqpcr()\u003c/code\u003e \u0026#x3082;\u0026#x3057;\u0026#x304F;\u0026#x306F; \u003ccode\u003eknownqpcr_unpaired()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x304C;\u0026#x4F7F;\u0026#x3048;\u0026#x308B;\u0026#x3002;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306E;\u0026#x500B;\u0026#x6570;\u0026#x304C;\u0026#x6975;\u0026#x3081;\u0026#x3066;\u0026#x591A;\u0026#x3051;\u0026#x308C;\u0026#x3070;\u0026#x3001;\u0026#x672A;\u0026#x77E5;\u0026#x6570;\u0026#x3068;\u0026#x3057;\u0026#x3066; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x5185;\u0026#x90E8;\u0026#x3067;\u0026#x63A8;\u0026#x5B9A;\u0026#x3059;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x306F;\u0026#x4E0D;\u0026#x53EF;\u0026#x80FD;\u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x3044;\u0026#x304C;\u0026#x3001;\u0026#x3088;\u0026#x307B;\u0026#x3069;\u0026#x306E;\u0026#x7406;\u0026#x7531;\u0026#x304C;\u0026#x306A;\u0026#x3044;\u0026#x9650;\u0026#x308A;\u0026#x56FA;\u0026#x5B9A;\u0026#x5024;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x4EE3;\u0026#x5165;\u0026#x3059;\u0026#x3079;\u0026#x304D;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x305F;\u0026#x3060;\u0026#x3067;\u0026#x3055;\u0026#x3048; X^TW\u0026#x3001; X^HW \u0026#x304C;\u0026#x7121;\u0026#x3044;\u0026#x3053;\u0026#x3068;\u0026#x306F;\u0026#x63A8;\u0026#x5B9A;\u0026#x6642;\u0026#x306E;\u0026#x30C7;\u0026#x30FC;\u0026#x30BF;\u0026#x70B9;\u0026#x304C;\u0026#x534A;\u0026#x5206;\u0026#x306B;\u0026#x306A;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x3092;\u0026#x610F;\u0026#x5473;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x308A;\u0026#x3001;\u0026#x672A;\u0026#x77E5;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x304C;\u0026#x591A;\u0026#x3044;\u0026#x3068;\u0026#x63A8;\u0026#x5B9A;\u0026#x7D50;\u0026#x679C;\u0026#x306B;\u0026#x5927;\u0026#x304D;\u0026#x306A;\u0026#x30D0;\u0026#x30A4;\u0026#x30A2;\u0026#x30B9;\u0026#x304C;\u0026#x751F;\u0026#x3058;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp align=\"center\"\u003e\n\u003cimg src=\"./vignettes/figures/freqpcr_vignette_fig05.png\" style=\"width: 50%; height: 50%\" alt=\"Figure 4. \u0026#x4E00;\u0026#x822C;\u0026#x7684;\u0026#x306A; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304F;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x4E2D;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x306E;\u0026#x6E2C;\u0026#x5B9A;\"\u003e\n\u003c/p\u003e\n\u003cblockquote\u003e\n\u003cp\u003eFigure 5. \u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x4E2D;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#x306E;\u0026#x6E2C;\u0026#x5B9A;\u0026#xFF1A;(3) \u0026#x394;Cq \u0026#x6CD5;\u0026#x3002;\u003c/p\u003e\n\u003c/blockquote\u003e\n\u003ch2 class=\"mume-header\" id=\"%E8%A4%87%E6%95%B0%E3%83%90%E3%83%AB%E3%82%AF%E3%82%B5%E3%83%B3%E3%83%97%E3%83%AB%E3%82%92%E7%94%A8%E3%81%84%E3%81%9F%E3%82%A2%E3%83%AA%E3%83%AB%E9%A0%BB%E5%BA%A6%E3%81%AE%E5%8C%BA%E9%96%93%E6%8E%A8%E5%AE%9A\"\u003e\u0026#x8907;\u0026#x6570;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x533A;\u0026#x9593;\u0026#x63A8;\u0026#x5B9A;\u003c/h2\u003e\n\n\u003cp\u003eOsakabe et al. (2017) \u0026#x3068; Maeoka et al. (2020) \u0026#x306E;\u0026#x5404;\u0026#x8AD6;\u0026#x6587;\u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x5404;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x69D8;\u0026#x3005;\u0026#x306A;\u0026#x6761;\u0026#x4EF6;\u0026#x3067;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x3092;\u0026#x884C;\u0026#x3044;\u0026#x3001;\u0026#x8A08;\u0026#x7B97;\u0026#x3057;\u0026#x305F; \u0026#x394;\u0026#x394;Cq \u0026#x5024;\u0026#x3092;\u0026#x3001;\u0026#x305D;\u0026#x306E;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x5360;\u0026#x3081;\u0026#x308B;\u0026#x5F53;\u0026#x8A72;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x542B;\u0026#x6709;\u0026#x6BD4;\u0026#xFF08;\u0026#x70B9;\u0026#x63A8;\u0026#x5B9A;\u0026#x5024;\u0026#xFF09;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u0026#x4E00;\u0026#x65B9; \u003ccode\u003efreqpcr\u003c/code\u003e \u0026#x30D1;\u0026#x30C3;\u0026#x30B1;\u0026#x30FC;\u0026#x30B8;\u0026#x306F; Cq \u0026#x5024;\u0026#x306B;\u0026#x57FA;\u0026#x3065;\u0026#x304F;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x306E;\u0026#x533A;\u0026#x9593;\u0026#x63A8;\u0026#x5B9A;\u0026#x3092;\u0026#x76EE;\u0026#x6307;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x304C;\u0026#x3001;\u0026#x305D;\u0026#x306E;\u0026#x305F;\u0026#x3081;\u0026#x306B;\u0026#x306F; 1 \u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x304B;\u0026#x3089;\u0026#x53D6;\u0026#x3089;\u0026#x308C;\u0026#x305F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x304C;\u0026#x8907;\u0026#x6570;\u0026#xFF08;N\u0026#xFF09;\u0026#x5FC5;\u0026#x8981;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x4E00;\u0026#x7B87;\u0026#x6240;\u0026#x306B;\u0026#x30C8;\u0026#x30E9;\u0026#x30C3;\u0026#x30D7;\u0026#x3092;\u0026#x8907;\u0026#x6570;\u0026#x8A2D;\u0026#x7F6E;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x30C8;\u0026#x30E9;\u0026#x30C3;\u0026#x30D7;\u0026#x3054;\u0026#x3068;\u0026#x306B; DNA \u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x305F;\u0026#x3082;\u0026#x306E;\u0026#x3092; 1 \u0026#x3064;\u0026#x306E;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3082;\u0026#x3088;\u0026#x3044;\u0026#x3057;\u0026#x3001;\u0026#x3042;\u0026#x308B;\u0026#x3044;\u0026#x306F;\u0026#x591A;\u0026#x6570;\u0026#x306E;\u0026#x6355;\u0026#x7372;\u0026#x500B;\u0026#x4F53;\u0026#x3092; n \u0026#x500B;\u0026#x4F53;\u0026#x305A;\u0026#x3064;\u0026#x307E;\u0026#x3068;\u0026#x3081;\u0026#x3066; DNA \u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x3066;\u0026#x3082;\u0026#x3088;\u0026#x3044;\u0026#x3002;\u003ccode\u003efreqpcr\u003c/code\u003e \u0026#x3067;\u0026#x306F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3054;\u0026#x3068;\u0026#x306B;\u0026#x500B;\u0026#x4F53;\u0026#x6570; n_h (h = 1, 2, \u0026#x2026;, N) \u0026#x304C;\u0026#x7570;\u0026#x306A;\u0026#x3063;\u0026#x3066;\u0026#x3082;\u0026#x3088;\u0026#x3044;\u0026#x304C;\u0026#x3001;\u0026#x5404;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306B;\u0026#x4F55;\u0026#x500B;\u0026#x4F53;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#x304B;\u0026#x306E;\u0026#x8A18;\u0026#x9332;\u0026#x306F;\u0026#x5FC5;\u0026#x9808;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x6355;\u0026#x7372;\u0026#x3057;\u0026#x305F;\u0026#x7DCF;\u0026#x500B;\u0026#x4F53;\u0026#x6570;\u0026#x304C;\u0026#x7B49;\u0026#x3057;\u0026#x3051;\u0026#x308C;\u0026#x3070;\u0026#x3001;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3092;\u0026#x591A;\u0026#x304F;\u0026#x5206;\u0026#x5272;\u0026#x3059;\u0026#x308B;\u0026#xFF08;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3042;\u0026#x305F;\u0026#x308A;\u0026#x306E;\u0026#x500B;\u0026#x4F53;\u0026#x6570;\u0026#x3092;\u0026#x5C11;\u0026#x306A;\u0026#x304F;\u0026#x3059;\u0026#x308B;\u0026#xFF09;\u0026#x307B;\u0026#x3069;\u0026#x4FE1;\u0026#x983C;\u0026#x533A;\u0026#x9593;\u0026#x306E;\u0026#x5E45;\u0026#x306F;\u0026#x72ED;\u0026#x304F;\u0026#x306A;\u0026#x308A;\u0026#x3001;\u0026#x9AD8;\u0026#x3044;\u0026#x63A8;\u0026#x5B9A;\u0026#x7CBE;\u0026#x5EA6;\u0026#x3092;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u0026#x3057;\u0026#x304B;\u0026#x3057;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x306E;\u0026#x691C;\u0026#x67FB;\u0026#x6570;\u0026#x304C;\u0026#x5897;\u0026#x3048;\u0026#x308B;\u0026#x306E;\u0026#x3067;\u0026#x30B3;\u0026#x30B9;\u0026#x30C8;\u0026#x306F;\u0026#x5897;\u0026#x5927;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306E;\u0026#x5206;\u0026#x5272;\u0026#x6570;\u0026#x306B;\u0026#x5FDC;\u0026#x3058;\u0026#x305F;\u0026#x63A8;\u0026#x5B9A;\u0026#x7CBE;\u0026#x5EA6;\u0026#x306E;\u0026#x30B7;\u0026#x30DF;\u0026#x30E5;\u0026#x30EC;\u0026#x30FC;\u0026#x30B7;\u0026#x30E7;\u0026#x30F3;\u0026#x7D50;\u0026#x679C;\u0026#x306F;\u0026#x3001;\u003ca href=\"https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13554#men13554-sec-0015-title\"\u003e\u0026#x539F;\u0026#x8457;\u0026#x8AD6;\u0026#x6587;\u0026#x306E; Figure 4\u003c/a\u003e \u0026#x304A;\u0026#x3088;\u0026#x3073; \u003ca href=\"https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13554#support-information-section\"\u003eESM1\u003c/a\u003e \u0026#x306B;\u0026#x793A;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003chr\u003e\n\u003ch2 class=\"mume-header\" id=\"2-%E5%80%8D%E4%BD%93%E3%81%AE%E5%8F%96%E6%89%B1%E3%81%84%E3%81%AB%E3%81%A4%E3%81%84%E3%81%A6\"\u003e2 \u0026#x500D;\u0026#x4F53;\u0026#x306E;\u0026#x53D6;\u0026#x6271;\u0026#x3044;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u003c/h2\u003e\n\n\u003cp\u003e\u003ccode\u003efreqpcr\u003c/code\u003e \u0026#x306E;\u0026#x30E2;\u0026#x30C7;\u0026#x30EB;\u0026#x69CB;\u0026#x9020;\u0026#x306F;\u0026#x3001;\u0026#x57FA;\u0026#x672C;\u0026#x7684;\u0026#x306B;\u0026#x306F;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x751F;\u0026#x7269;\u0026#x3092;\u0026#x4EEE;\u0026#x5B9A;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3002;\u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061;\u0026#x5404;\u0026#x500B;\u0026#x4F53;\u0026#x3092;\u0026#x69CB;\u0026#x6210;\u0026#x3059;\u0026#x308B;\u0026#x5404;\u0026#x7D30;\u0026#x80DE;\u0026#x304C; 1 \u0026#x672C;\u0026#x306E;\u0026#x67D3;\u0026#x8272;\u0026#x4F53;\u0026#x3092;\u0026#x6301;\u0026#x3061;\u0026#x3001;1 \u0026#x3064;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB; DNA \u0026#x3092;\u0026#x542B;\u0026#x3080;\u0026#x3002;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6;\u0026#x63A8;\u0026#x5B9A;\u0026#x3092;\u0026#x884C;\u0026#x3046; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x306F; \u003ccode\u003ediploid\u003c/code\u003e \u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x30AA;\u0026#x30D7;\u0026#x30B7;\u0026#x30E7;\u0026#x30F3;\u0026#x5909;\u0026#x6570;\u0026#x3092;\u0026#x6301;\u0026#x3061;\u0026#x3001;\u0026#x521D;\u0026#x671F;\u0026#x8A2D;\u0026#x5B9A;\u0026#x306F; \u003ccode\u003ediploid = FALSE\u003c/code\u003e \u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x5404;\u0026#x500B;\u0026#x4F53;\u0026#x306E; DNA \u0026#x91CF;\u0026#x3092;\u0026#x30E2;\u0026#x30C7;\u0026#x30EA;\u0026#x30F3;\u0026#x30B0;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x901A;\u0026#x5E38;\u0026#x306E;\u0026#x6606;\u0026#x866B;\u0026#x3084;\u0026#x810A;\u0026#x690E;\u0026#x52D5;\u0026#x7269;\u0026#x306F; 2\u0026#x500D;\u0026#x4F53;\u0026#x3001;\u0026#x3059;\u0026#x306A;\u0026#x308F;\u0026#x3061;\u0026#x5404;\u0026#x7D30;\u0026#x80DE;\u0026#x304C; 2 \u0026#x672C;\u0026#x306E;\u0026#x67D3;\u0026#x8272;\u0026#x4F53;\u0026#x3092;\u0026#x6301;\u0026#x3064;\u0026#x3002;\u0026#x3053;\u0026#x306E;\u0026#x5834;\u0026#x5408;\u0026#x3001;\u003ccode\u003ediploid = TRUE\u003c/code\u003e \u0026#x3092;\u0026#x8A2D;\u0026#x5B9A;\u0026#x3057;\u0026#x3066; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x3092;\u0026#x5B9F;\u0026#x884C;\u0026#x3059;\u0026#x308C;\u0026#x3070;\u0026#x3001;\u0026#x7C21;\u0026#x6613;\u0026#x7684;\u0026#x306B; 2 \u0026#x500D;\u0026#x4F53;\u0026#x751F;\u0026#x7269;\u0026#x3092;\u0026#x53D6;\u0026#x308A;\u0026#x6271;\u0026#x3046;\u0026#x3053;\u0026#x3068;\u0026#x304C;\u0026#x3067;\u0026#x304D;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x3067;\u0026#x306F; \u003ccode\u003eN\u003c/code\u003e \u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x5909;\u0026#x6570;\u0026#x30D9;\u0026#x30AF;\u0026#x30C8;\u0026#x30EB;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3092;\u0026#x69CB;\u0026#x6210;\u0026#x3059;\u0026#x308B;\u0026#x500B;\u0026#x4F53;\u0026#x306E;\u0026#x6570;\u0026#x3092;\u0026#x4EE3;\u0026#x5165;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003ccode\u003ediploid = TRUE\u003c/code\u003e \u0026#x3067;\u0026#x3082; \u003ccode\u003ediploid = FALSE\u003c/code\u003e \u0026#x3067;\u0026#x3082;\u0026#x3001;\u003ccode\u003eN\u003c/code\u003e \u0026#x306F;\u0026#x67D3;\u0026#x8272;\u0026#x4F53;\u0026#x6570;\u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x304F;\u0026#x500B;\u0026#x4F53;\u0026#x6570;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x4EE3;\u0026#x5165;\u0026#x3059;\u0026#x308B;\u0026#x4ED5;\u0026#x69D8;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x305F;\u0026#x3068;\u0026#x3048;\u0026#x3070; \u003ccode\u003eN = c(8, 8, 8, 6)\u003c/code\u003e \u0026#x3068;\u0026#x4EE3;\u0026#x5165;\u0026#x3059;\u0026#x308B;\u0026#x3068;\u0026#x3001;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x3067;\u0026#x3042;\u0026#x3063;\u0026#x3066;\u0026#x3082; 2 \u0026#x500D;\u0026#x4F53;\u0026#x3067;\u0026#x3042;\u0026#x3063;\u0026#x3066;\u0026#x3082;\u0026#x5408;\u0026#x8A08; 30 \u0026#x500B;\u0026#x4F53;\u0026#x304C;\u0026#x63A1;\u0026#x96C6;\u0026#x3055;\u0026#x308C;\u0026#x3001;\u0026#x3053;\u0026#x308C;\u0026#x3092; 8 \u0026#x500B;\u0026#x4F53;\u0026#x305A;\u0026#x3064; DNA \u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x305F;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x6EB6;\u0026#x6DB2; 3 \u0026#x672C;\u0026#x3068;\u0026#x3001;\u0026#x6B8B;\u0026#x308B; 6 \u0026#x500B;\u0026#x4F53;\u0026#x3092; DNA \u0026#x62BD;\u0026#x51FA;\u0026#x3057;\u0026#x305F;\u0026#x6EB6;\u0026#x6DB2; 1 \u0026#x672C;\u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x69CB;\u0026#x6210;\u0026#x3067;\u0026#x5206;\u0026#x6790;\u0026#x3055;\u0026#x308C;\u0026#x305F;\u0026#x3053;\u0026#x3068;\u0026#x3092;\u0026#x610F;\u0026#x5473;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e2 \u0026#x500D;\u0026#x4F53;\u0026#x751F;\u0026#x7269;\u0026#x306B;\u0026#x304A;\u0026#x3051;\u0026#x308B;\u0026#x500B;\u0026#x4F53;\u0026#x3042;\u0026#x305F;\u0026#x308A; DNA \u0026#x53CE;\u0026#x91CF;\u0026#x306E;\u0026#x30E2;\u0026#x30C7;\u0026#x30EB;\u0026#x8868;\u0026#x73FE;\u0026#x3092;\u0026#x3001;\u0026#x73FE;\u0026#x884C;\u0026#x306E; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x3067;\u0026#x306F;\u0026#x7C21;\u0026#x7565;\u0026#x5316;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x306B;\u0026#x6CE8;\u0026#x610F;\u0026#x304C;\u0026#x5FC5;\u0026#x8981;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x8A73;\u0026#x7D30;\u0026#x306F;\u0026#x5143;\u0026#x8AD6;\u0026#x6587;\u0026#x306E; Appendix S1 \u0026#x306B;\u0026#x8A18;\u0026#x8FF0;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x304C;\u0026#x3001;\u0026#x7AEF;\u0026#x7684;\u0026#x306B;\u0026#x3044;\u0026#x3046;\u0026#x3068;\u0026#x540C;\u0026#x4E00;\u0026#x500B;\u0026#x4F53;\u0026#x304C;\u0026#x6301;\u0026#x3064; 2 \u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x5B9A;\u0026#x91CF;\u0026#x304C;\u0026#x53B3;\u0026#x5BC6;\u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x3044;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u0026#x672C;\u0026#x6765;\u0026#x3001;2 \u0026#x500D;\u0026#x4F53;\u0026#x306E; 1 \u0026#x500B;\u0026#x4F53;\u0026#x304B;\u0026#x3089;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB; DNA \u0026#x91CF;\u0026#x306F;\u0026#x3001;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x5F53;\u0026#x91CF;\u0026#x306E;\u0026#x3061;\u0026#x3087;\u0026#x3046;\u0026#x3069; 2 \u0026#x500D;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x3068;\u0026#x308A;\u0026#x308F;\u0026#x3051;\u0026#x30D8;\u0026#x30C6;\u0026#x30ED;\u0026#x500B;\u0026#x4F53;\u0026#x304C;\u0026#x6301;\u0026#x3064; R \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3068; S \u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E; DNA \u0026#x91CF;\u0026#x306F;\u0026#x540C;\u0026#x4E00;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3079;\u0026#x304D;\u0026#x3060;\u0026#x304C;\u0026#x3001;\u0026#x3053;\u0026#x306E;\u0026#x5236;\u0026#x7D04;\u0026#x6761;\u0026#x4EF6;\u0026#x3092;\u0026#x7F6E;\u0026#x304F;\u0026#x3068;\u0026#x30D7;\u0026#x30ED;\u0026#x30B0;\u0026#x30E9;\u0026#x30E0;\u0026#x306E;\u0026#x8A08;\u0026#x7B97;\u0026#x91CF;\u0026#x304C;\u0026#x8457;\u0026#x3057;\u0026#x304F;\u0026#x5897;\u0026#x5927;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x305D;\u0026#x3053;\u0026#x3067;\u0026#x300C;2 \u0026#x500D;\u0026#x4F53;\u0026#x751F;\u0026#x7269;\u0026#x3092; n \u0026#x500B;\u0026#x4F53;\u0026#x63A1;\u0026#x96C6;\u0026#x3059;\u0026#x308B;\u0026#x300D;\u0026#x4EE3;\u0026#x308F;\u0026#x308A;\u0026#x306B;\u0026#x3001;\u0026#x300C;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x751F;\u0026#x7269;\u0026#x3092; 2n \u0026#x500B;\u0026#x4F53;\u0026#x63A1;\u0026#x96C6;\u0026#x3057;\u0026#x305F;\u0026#x3053;\u0026#x3068;\u0026#x306B;\u0026#x3059;\u0026#x308B;\u0026#x300D;\u0026#x30C8;\u0026#x30EA;\u0026#x30C3;\u0026#x30AF;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F;\u0026#x3002;\u0026#x3053;\u0026#x308C;\u0026#x306B;\u0026#x3088;\u0026#x308A;\u0026#x3001;\u0026#x540C;\u0026#x4E00;\u0026#x500B;\u0026#x4F53;\u0026#x304B;\u0026#x3089;\u0026#x5F97;\u0026#x3089;\u0026#x308C;\u0026#x305F; 2 \u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x5206;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E; DNA \u0026#x91CF;\u0026#x304C;\u0026#x3001;\u0026#x305D;\u0026#x308C;\u0026#x305E;\u0026#x308C;\u0026#x306E;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u0026#x72EC;\u0026#x7ACB;\u0026#x540C;\u0026#x6642;\u0026#x306A;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x306B;\u0026#x5F93;\u0026#x3046;\u0026#x3088;\u0026#x3046;\u0026#x306B;\u0026#x306A;\u0026#x308A;\u0026#x3001;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x3068;\u0026#x540C;\u0026#x3058;\u0026#x30E2;\u0026#x30C7;\u0026#x30EB;\u0026#x3092;\u0026#x9069;\u0026#x7528;\u0026#x3067;\u0026#x304D;\u0026#x308B;\u0026#x3002;\u0026#x672C;\u0026#x7C21;\u0026#x6613;\u0026#x63A8;\u0026#x5B9A;\u0026#x6CD5;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x3067;\u0026#x3001;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x983B;\u0026#x5EA6; p \u0026#x306E;\u0026#x6700;\u0026#x5C24;\u0026#x63A8;\u0026#x5B9A;\u0026#x5024;\u0026#xFF08;\u0026#x70B9;\u0026#x63A8;\u0026#x5B9A;\u0026#x5024;\u0026#xFF09;\u0026#x306B;\u0026#x306F;\u0026#x5F71;\u0026#x97FF;\u0026#x3057;\u0026#x306A;\u0026#x3044;\u0026#x3068;\u0026#x8003;\u0026#x3048;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x304C;\u0026#x3001;\u0026#x4FE1;\u0026#x983C;\u0026#x533A;\u0026#x9593;\u0026#x304C;\u0026#x672C;\u0026#x6765;\u0026#x306E;\u0026#x7BC4;\u0026#x56F2;\u0026#x3088;\u0026#x308A;\u0026#x3082;\u0026#x50C5;\u0026#x304B;\u0026#x306B;\u0026#x5E83;\u0026#x304F;\u0026#x63A8;\u0026#x5B9A;\u0026#x3055;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003chr\u003e\n\u003ch2 class=\"mume-header\" id=\"%E9%80%A3%E7%B6%9A%E3%81%AA-dna-%E9%87%8F%E3%82%92%E4%BB%AE%E5%AE%9A%E3%81%97%E3%81%9F-freqpcr-%E3%83%A2%E3%83%87%E3%83%AB%E3%81%AB%E3%81%A4%E3%81%84%E3%81%A6\"\u003e\u0026#x9023;\u0026#x7D9A;\u0026#x306A; DNA \u0026#x91CF;\u0026#x3092;\u0026#x4EEE;\u0026#x5B9A;\u0026#x3057;\u0026#x305F; freqpcr \u0026#x30E2;\u0026#x30C7;\u0026#x30EB;\u0026#x306B;\u0026#x3064;\u0026#x3044;\u0026#x3066;\u003c/h2\u003e\n\n\u003cp\u003e\u0026#xFF08;\u0026#x57F7;\u0026#x7B46;\u0026#x4E2D;\u0026#xFF09;\u003c/p\u003e\n\u003chr\u003e\n\u003ch1 class=\"mume-header\" id=\"%E4%BE%9D%E5%AD%98%E3%83%91%E3%83%83%E3%82%B1%E3%83%BC%E3%82%B8\"\u003e\u0026#x4F9D;\u0026#x5B58;\u0026#x30D1;\u0026#x30C3;\u0026#x30B1;\u0026#x30FC;\u0026#x30B8;\u003c/h1\u003e\n\n\u003cul\u003e\n\u003cli\u003eR (\u0026gt;= 3.6)\u003c/li\u003e\n\u003cli\u003ecubature (\u003ca href=\"https://cran.r-project.org/package=cubature\"\u003ehttps://cran.r-project.org/package=cubature\u003c/a\u003e)\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u0026#x958B;\u0026#x767A;\u0026#x74B0;\u0026#x5883;\u0026#x306F; Windows 10 \u0026#x4E0A;\u0026#x306E; R 3.6 \u0026#x3060;\u0026#x304C;\u0026#x3001;R 4.1.2 \u0026#x3067;\u0026#x3082;\u0026#x52D5;\u0026#x4F5C;\u0026#x3057;\u0026#x305F;\u0026#x3068;\u0026#x306E;\u0026#x3053;\u0026#x3068;\u0026#x3002;\u003c/p\u003e\n\u003ch1 class=\"mume-header\" id=\"%E3%82%A4%E3%83%B3%E3%82%B9%E3%83%88%E3%83%BC%E3%83%AB\"\u003e\u0026#x30A4;\u0026#x30F3;\u0026#x30B9;\u0026#x30C8;\u0026#x30FC;\u0026#x30EB;\u003c/h1\u003e\n\n\u003cpre data-role=\"codeBlock\" data-info=\"R\" class=\"language-r\"\u003einstall.packages\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003e\u003cspan class=\"token string\"\u003e\u0026quot;freqpcr\u0026quot;\u003c/span\u003e\u003cspan class=\"token punctuation\"\u003e,\u003c/span\u003e dependencies\u003cspan class=\"token operator\"\u003e=\u003c/span\u003e\u003cspan class=\"token boolean\"\u003eTRUE\u003c/span\u003e\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\n\nlibrary\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003efreqpcr\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\npackageVersion\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003e\u003cspan class=\"token string\"\u003e\u0026quot;freqpcr\u0026quot;\u003c/span\u003e\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\n\u003c/pre\u003e\u003cp\u003e\u0026#x3082;\u0026#x3057;\u0026#x304F;\u0026#x306F;\u003c/p\u003e\n\u003cpre data-role=\"codeBlock\" data-info=\"R\" class=\"language-r\"\u003elibrary\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003eremotes\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\ninstall_github\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003e\u003cspan class=\"token string\"\u003e\u0026quot;sudoms/freqpcr\u0026quot;\u003c/span\u003e\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\n\nlibrary\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003efreqpcr\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\npackageVersion\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003e\u003cspan class=\"token string\"\u003e\u0026quot;freqpcr\u0026quot;\u003c/span\u003e\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\n\u003c/pre\u003e\u003cp\u003e\u0026#x4EE5;\u0026#x4E0B;\u0026#x306E;\u0026#x3088;\u0026#x3046;\u0026#x306A;\u0026#x30A8;\u0026#x30E9;\u0026#x30FC;\u0026#x30E1;\u0026#x30C3;\u0026#x30BB;\u0026#x30FC;\u0026#x30B8;\u0026#x304C;\u0026#x51FA;\u0026#x3066;\u0026#x3001;\u0026#x30A4;\u0026#x30F3;\u0026#x30B9;\u0026#x30C8;\u0026#x30FC;\u0026#x30EB;\u0026#x306B;\u0026#x5931;\u0026#x6557;\u0026#x3059;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x304C;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cpre data-role=\"codeBlock\" data-info=\"R\" class=\"language-r\"\u003e\u003cspan class=\"token operator\"\u003e*\u003c/span\u003e\u003cspan class=\"token operator\"\u003e*\u003c/span\u003e byte\u003cspan class=\"token operator\"\u003e-\u003c/span\u003ecompile and prepare package \u003cspan class=\"token keyword keyword-for\"\u003efor\u003c/span\u003e lazy loading\nError\u003cspan class=\"token operator\"\u003e:\u003c/span\u003e \u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003econverted from warning\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e package \u003cspan class=\"token string\"\u003e\u0026apos;cubature\u0026apos;\u003c/span\u003e was built under R version \u003cspan class=\"token number\"\u003e3.6\u003c/span\u003e\u003cspan class=\"token number\"\u003e.3\u003c/span\u003e\n\u003c/pre\u003e\u003cp\u003e\u0026#x4F9D;\u0026#x5B58;\u0026#x30D1;\u0026#x30C3;\u0026#x30B1;\u0026#x30FC;\u0026#x30B8; \u0026#x2018;cubature\u0026#x2019; \u0026#x304C;\u0026#x3001;\u003ca href=\"https://github.com/r-lib/remotes/issues/403\"\u003e\u0026#x30E6;\u0026#x30FC;\u0026#x30B6;\u0026#x30FC;\u0026#x306E;\u0026#x74B0;\u0026#x5883;\u0026#x3088;\u0026#x308A;\u0026#x3082;\u0026#x65B0;\u0026#x3057;\u0026#x3044;\u0026#x30D0;\u0026#x30FC;\u0026#x30B8;\u0026#x30E7;\u0026#x30F3;\u0026#x306E; R \u0026#x3067;\u0026#x30B3;\u0026#x30F3;\u0026#x30D1;\u0026#x30A4;\u0026#x30EB;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u003c/a\u003e\u0026#x5834;\u0026#x5408;\u0026#x306B;\u0026#x3001;\u0026#x3053;\u0026#x306E;\u0026#x30A8;\u0026#x30E9;\u0026#x30FC;\u0026#x304C;\u0026#x51FA;\u0026#x308B;\u0026#x3002;\nR \u0026#x306E;\u0026#x30D0;\u0026#x30FC;\u0026#x30B8;\u0026#x30E7;\u0026#x30F3;\u0026#x3092;\u0026#x7D20;\u0026#x76F4;\u0026#x306B;\u0026#x6700;\u0026#x65B0;\u0026#x7248;\u0026#x306B;\u0026#x3059;\u0026#x308B;\u0026#x3001;\u0026#x3082;\u0026#x3057;\u0026#x304F;\u0026#x306F;\u0026#x74B0;\u0026#x5883;\u0026#x5909;\u0026#x6570; \u003ccode\u003eR_REMOTES_NO_ERRORS_FROM_WARNINGS=\u0026quot;true\u0026quot;\u003c/code\u003e \u0026#x3092;\u0026#x8A2D;\u0026#x5B9A;\u0026#x5F8C;\u0026#x3001;\u003ccode\u003einstall_github()\u003c/code\u003e \u0026#x3092;\u0026#x518D;\u0026#x5EA6;\u0026#x8D70;\u0026#x3089;\u0026#x305B;\u0026#x308B;\u0026#x3053;\u0026#x3068;\u0026#x3067;\u0026#x89E3;\u0026#x6D88;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cpre data-role=\"codeBlock\" data-info=\"R\" class=\"language-r\"\u003eSys.setenv\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003eR_REMOTES_NO_ERRORS_FROM_WARNINGS\u003cspan class=\"token operator\"\u003e=\u003c/span\u003e\u003cspan class=\"token string\"\u003e\u0026quot;true\u0026quot;\u003c/span\u003e\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\ninstall_github\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003e\u003cspan class=\"token string\"\u003e\u0026quot;sudoms/freqpcr\u0026quot;\u003c/span\u003e\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\n\u003c/pre\u003e\u003ch1 class=\"mume-header\" id=\"%E4%BD%BF%E7%94%A8%E6%B3%95\"\u003e\u0026#x4F7F;\u0026#x7528;\u0026#x6CD5;\u003c/h1\u003e\n\n\u003cpre data-role=\"codeBlock\" data-info=\"R\" class=\"language-r\"\u003elibrary\u003cspan class=\"token punctuation\"\u003e(\u003c/span\u003efreqpcr\u003cspan class=\"token punctuation\"\u003e)\u003c/span\u003e\n\u003c/pre\u003e\u003ch2 class=\"mume-header\" id=\"%E5%A4%89%E6%95%B0%E3%83%91%E3%83%A9%E3%83%A1%E3%83%BC%E3%82%BF%E3%81%AE%E4%B8%80%E8%A6%A7\"\u003e\u0026#x5909;\u0026#x6570;\u0026#x30FB;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x306E;\u0026#x4E00;\u0026#x89A7;\u003c/h2\u003e\n\n\u003cp\u003eP:\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u0026#x63A8;\u0026#x5B9A;\u0026#x5BFE;\u0026#x8C61;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x306B;\u0026#x5360;\u0026#x3081;\u0026#x308B;\u0026#x983B;\u0026#x5EA6;\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003eK:\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u0026#x5BFE;\u0026#x8C61;\u0026#x500B;\u0026#x4F53;\u0026#x7FA4;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x3001;1 \u0026#x500B;\u0026#x4F53;\u0026#xFF08;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x5F53;\u0026#x91CF;\u0026#xFF09;\u0026#x3042;\u0026#x305F;\u0026#x308A;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB; DNA \u0026#x53CE;\u0026#x91CF;\u0026#x3092;\u0026#x8FD1;\u0026#x4F3C;\u0026#x3059;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x306B;\u0026#x7528;\u0026#x3044;\u0026#x3089;\u0026#x308C;\u0026#x308B;\u0026#x30AC;\u0026#x30F3;\u0026#x30DE;\u0026#x5206;\u0026#x5E03;\u0026#x306E;\u0026#x5F62;\u0026#x72B6;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u0026#x4EE5;\u0026#x4E0B;\u0026#x306F;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x306E;\u0026#x5B9F;\u0026#x9A13;\u0026#x7CFB;\u0026#x306B;\u0026#x4F9D;\u0026#x5B58;\u0026#x3059;\u0026#x308B;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x6B21;\u0026#x306B;\u0026#x8AAC;\u0026#x660E;\u0026#x3059;\u0026#x308B; \u003ccode\u003eknownqpcr()\u003c/code\u003e \u0026#x304A;\u0026#x3088;\u0026#x3073; \u003ccode\u003eknownqpcr_unpaired()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x306E;\u0026#x3044;\u0026#x305A;\u0026#x308C;\u0026#x304B;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x4E88;\u0026#x3081;\u0026#x63A8;\u0026#x5B9A;\u0026#x3057;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x3002;\u0026#x306A;\u0026#x304A; \u003cstrong\u003e\u0026#x592A;\u0026#x5B57;\u003c/strong\u003e \u0026#x306F; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x306E;\u0026#x52D5;\u0026#x4F5C;\u0026#x6642;\u0026#x306B;\u0026#x30E6;\u0026#x30FC;\u0026#x30B6;\u0026#x30FC;\u0026#x306B;\u0026#x3088;\u0026#x308B;\u0026#x5024;\u0026#x306E;\u0026#x6307;\u0026#x5B9A;\u0026#x304C;\u0026#x5FC5;\u0026#x9808;\u0026#x306E;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x3001; \u003cem\u003e\u003cstrong\u003e\u0026#x592A;\u0026#x5B57;\u0026#x304B;\u0026#x3064;\u0026#x659C;\u0026#x4F53;\u003c/strong\u003e\u003c/em\u003e \u0026#x306F;\u0026#x5FC5;\u0026#x9808;\u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x3044;\u0026#x304C;\u0026#x3001;\u0026#x53EF;\u0026#x80FD;\u0026#x306A;\u0026#x9650;\u0026#x308A;\u0026#x6307;\u0026#x5B9A;\u0026#x3059;\u0026#x3079;\u0026#x304D;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x7279;\u0026#x306B;\u0026#x30D0;\u0026#x30EB;\u0026#x30AF;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x306E;\u0026#x6570;\u0026#x304C;\u0026#x5C11;\u0026#x306A;\u0026#x3044;\u0026#x6642;\u0026#x306B;\u0026#x3001;\u003ccode\u003esdMeasure\u003c/code\u003e \u0026#x3092;\u0026#x6307;\u0026#x5B9A;\u0026#x3057;\u0026#x306A;\u0026#x3044;\u0026#x3068;\u0026#x63A8;\u0026#x5B9A;\u0026#x7D50;\u0026#x679C;\u0026#x304C;\u0026#x5927;\u0026#x304D;\u0026#x304F;\u0026#x305A;\u0026#x308C;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEPCR\u003c/strong\u003e:\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003ePCR \u0026#x30B5;\u0026#x30A4;\u0026#x30AF;\u0026#x30EB;\u0026#x3054;\u0026#x3068;\u0026#x306E;\u0026#x5897;\u0026#x5E45;\u0026#x52B9;\u0026#x7387;\u0026#x3092;\u0026#x8868;\u0026#x3059;\u0026#x6B63;\u0026#x306E;\u0026#x5B9F;\u0026#x6570;\u0026#x3002;\u003c/li\u003e\n\u003cli\u003eEPCR = 1 \u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3068;\u0026#x304D;\u0026#x3001;\u0026#x92F3;\u0026#x578B; DNA \u0026#x304C; PCR 1 \u0026#x30B5;\u0026#x30A4;\u0026#x30AF;\u0026#x30EB;\u0026#x3042;\u0026#x305F;\u0026#x308A; 2 \u0026#x500D;\u0026#x305A;\u0026#x3064;\u0026#x5897;\u0026#x3048;\u0026#x308B;\u0026#xFF08;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x306E;\u0026#x6163;\u0026#x7FD2;\u0026#x306B;\u0026#x5F93;\u0026#x3044;\u0026#x3001;EPCR + 1 = 2 \u0026#x306A;\u0026#x306E;\u0026#x3067; 2 \u0026#x500D;\u0026#x3068;\u0026#x5B9A;\u0026#x7FA9;\u0026#x3057;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#xFF09;\u0026#x3002;\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u003cstrong\u003ezeroAmount\u003c/strong\u003e:\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u0026#xFF08;RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#xFF09;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x3068;\u0026#x3059;\u0026#x308B;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x3067;\u0026#x306A;\u0026#x3044;\u0026#x65B9;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x3092;\u0026#x5206;\u0026#x89E3;\u0026#x3059;\u0026#x308B;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x53CD;\u0026#x5FDC;\u0026#x3067;\u0026#x3001;\u0026#x53CD;\u0026#x5FDC;\u0026#x5F8C;\u0026#x3082;\u0026#x306A;\u0026#x304A;\u0026#x5206;\u0026#x89E3;\u0026#x3055;\u0026#x308C;\u0026#x306A;\u0026#x304B;\u0026#x3063;\u0026#x305F;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB; DNA \u0026#x306E;\u0026#x6B8B;\u0026#x5B58;\u0026#x7387;\u0026#x3002;\u003c/p\u003e\n\u003c/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u0026#xFF08;\u0026#x4E00;\u0026#x822C;\u0026#x7684;\u0026#x306A; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#xFF09;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x7279;\u0026#x7570;\u0026#x7684;\u0026#x306A; PCR \u0026#x30D7;\u0026#x30E9;\u0026#x30A4;\u0026#x30DE;\u0026#x30FC;\u0026#x30BB;\u0026#x30C3;\u0026#x30C8;\u0026#x3092;\u0026#x7528;\u0026#x3044;\u0026#x305F; PCR \u0026#x53CD;\u0026#x5FDC;\u0026#x3067;\u0026#x3001;\u0026#x6975;\u0026#x50C5;\u0026#x304B;\u0026#x306B;\u0026#x5897;\u0026#x5E45;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3057;\u0026#x307E;\u0026#x3046;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x3067;\u0026#x306A;\u0026#x3044;\u0026#x65B9;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x5272;\u0026#x5408;\u0026#xFF08;\u0026#x691C;\u0026#x51FA;\u0026#x5BFE;\u0026#x8C61;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x92F3;\u0026#x578B;\u0026#x91CF;\u0026#x3092; 1 \u0026#x3068;\u0026#x3057;\u0026#x305F;\u0026#x3001;\u0026#x92F3;\u0026#x578B;\u0026#x5024;\u0026#x306E;\u0026#x76F8;\u0026#x5BFE;\u0026#x5024;\u0026#xFF09;\u0026#x3002;\u003c/p\u003e\n\u003c/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x306E;\u0026#x5B9F;\u0026#x884C;\u0026#x6642;\u0026#x306B;\u0026#x306F; 0 \u0026#x4EE5;\u0026#x4E0A; 1 \u0026#x4EE5;\u0026#x4E0B;\u0026#x306E;\u0026#x5B9F;\u0026#x6570;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x3001;\u0026#x30E6;\u0026#x30FC;\u0026#x30B6;\u0026#x30FC;\u0026#x304C;\u0026#x6307;\u0026#x5B9A;\u0026#x3059;\u0026#x308B;\u0026#x5FC5;\u0026#x8981;\u0026#x304C;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u003c/p\u003e\n\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003esdMeasure\u003c/strong\u003e\u003c/em\u003e:\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x5206;\u0026#x6790;\u0026#x306B;\u0026#x304A;\u0026#x3051;\u0026#x308B;\u0026#x5404; Cq (Ct) \u0026#x5024;\u0026#x304C;\u0026#x5F93;\u0026#x3046;\u0026#x30E9;\u0026#x30F3;\u0026#x30C0;\u0026#x30E0;\u0026#x8AA4;\u0026#x5DEE;\u0026#xFF08;\u0026#x6A19;\u0026#x6E96;\u0026#x504F;\u0026#x5DEE;\u0026#x306E;\u0026#x5927;\u0026#x304D;\u0026#x3055;\u0026#xFF09;\u0026#x3002;\u0026#x5143;\u0026#x8AD6;\u0026#x6587;\u0026#x3067; \u0026#x3C3;_c \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x8868;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x5909;\u0026#x6570;\u0026#x3002;\u0026#x30EA;\u0026#x30A2;\u0026#x30EB;\u0026#x30BF;\u0026#x30A4;\u0026#x30E0; PCR \u0026#x306E;\u0026#x5B9F;\u0026#x9A13;\u0026#x8AA4;\u0026#x5DEE;\u0026#x306F; Cq \u0026#x5024;\u0026#x306E;\u0026#x30B9;\u0026#x30B1;\u0026#x30FC;\u0026#x30EB;\u0026#x3067;\u0026#x6982;\u0026#x306D;\u0026#x6B63;\u0026#x898F;\u0026#x5206;\u0026#x5E03;\u0026#x3059;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#xFF08;\u0026#x672C;\u0026#x7814;\u0026#x7A76;\u0026#x306E;\u003ca href=\"https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13554#men13554-sec-0010-title\"\u003e\u0026#x539F;\u0026#x8457;\u0026#x8AD6;\u0026#x6587;\u0026#x306E;\u0026#x5B9F;\u0026#x9A13; 1\u003c/a\u003e \u0026#x3092;\u0026#x53C2;\u0026#x7167;\u0026#x305B;\u0026#x3088;\u0026#xFF09;\u0026#x3002;\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003etargetScale\u003c/strong\u003e\u003c/em\u003e:\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u0026#x540C;\u0026#x4E00;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x304B;\u0026#x3089;\u0026#x5206;\u0026#x6CE8;\u0026#x3057;\u0026#x305F;\u0026#x7B49;\u0026#x91CF;\u0026#x306E; DNA \u0026#x6EB6;\u0026#x6DB2;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x3068;\u0026#x3059;\u0026#x308B;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x5EA7;\u0026#x306E; DNA \u0026#x304C;\u0026#xFF08;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306B;\u0026#x95A2;\u0026#x4FC2;\u0026#x306A;\u0026#x304F;\u0026#xFF09;\u0026#x3001;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x306E;\u0026#x4F55;\u0026#x500D;\u0026#x5B58;\u0026#x5728;\u0026#x3059;\u0026#x308B;\u0026#x304B;\u0026#x3002;\u0026#x5143;\u0026#x8AD6;\u0026#x6587;\u0026#x3067; \u0026#x3B4;_T \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x8868;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x5909;\u0026#x6570;\u0026#x3002;\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003escaleDNA:\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e1 \u0026#x500B;\u0026#x4F53;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x308B;\u0026#xFF08;\u0026#x5358;\u0026#x6570;\u0026#x4F53;\u0026#x5F53;\u0026#x91CF;\u0026#x306E;\u0026#xFF09;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x306E;\u0026#x5E73;\u0026#x5747;\u0026#x7684;\u0026#x306A; DNA \u0026#x91CF;\u0026#x304C;\u0026#x3001;PCR \u0026#x53CD;\u0026#x5FDC;\u0026#x306E;\u0026#x30B9;\u0026#x30EC;\u0026#x30C3;\u0026#x30B7;\u0026#x30E5;\u0026#x30DB;\u0026#x30FC;\u0026#x30EB;\u0026#x30C9;\u0026#x306B;\u0026#x6BD4;\u0026#x3079;\u0026#x3066;\u0026#x4F55;\u0026#x500D;\u0026#x5B58;\u0026#x5728;\u0026#x3059;\u0026#x308B;\u0026#x304B;\u0026#x3002;PCR 1 \u0026#x30B5;\u0026#x30A4;\u0026#x30AF;\u0026#x30EB;\u0026#x3067;\u0026#x3061;\u0026#x3087;\u0026#x3046;\u0026#x3069; 2 \u0026#x500D;\u0026#x306B;\u0026#x5897;\u0026#x3048;\u0026#x308B;\u0026#xFF08;\u003ccode\u003eEPCR\u003c/code\u003e = 1\u0026#xFF09;\u0026#x306A;\u0026#x3089;\u0026#x3070;\u0026#x3001;\u003ccode\u003escaleDNA\u003c/code\u003e = 0.5 \u0026#x306E;\u0026#x3068;\u0026#x304D; 1 \u0026#x30B5;\u0026#x30A4;\u0026#x30AF;\u0026#x30EB;\u0026#x306E; PCR \u0026#x3067;\u0026#x30B9;\u0026#x30EC;\u0026#x30C3;\u0026#x30B7;\u0026#x30E5;\u0026#x30DB;\u0026#x30FC;\u0026#x30EB;\u0026#x30C9;\u0026#x306B;\u0026#x9054;\u0026#x3059;\u0026#x308B;\u0026#x3002;2^{10} = 1024 \u0026#x304B;\u0026#x3089;\u0026#x985E;\u0026#x63A8;\u0026#x3067;\u0026#x304D;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x306B;\u0026#x3001;\u003ccode\u003escaleDNA\u003c/code\u003e = 10^{-6} \u0026#x3067;\u0026#x3042;\u0026#x308C;\u0026#x3070;\u0026#x7D04; 20 \u0026#x306E; Cq \u0026#x5024;\u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u003c/li\u003e\n\u003cli\u003e\u0026#x306A;\u0026#x304A; \u003ccode\u003eEPCR\u003c/code\u003e \u0026#x304C;\u0026#x6C7A;\u0026#x307E;\u0026#x308C;\u0026#x3070;\u0026#x81EA;\u0026#x305A;\u0026#x3068;\u0026#x56FA;\u0026#x5B9A;\u0026#x3059;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x3001;\u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x306E;\u0026#x5F15;\u0026#x6570;\u0026#x306B;\u0026#x306F;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x306A;\u0026#x3044;\u0026#x3002;\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003ebaseChange:\u003c/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eRED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x3092;\u0026#x884C;\u0026#x3046;\u0026#x524D;\u0026#x5F8C;\u0026#x3067;\u0026#x3001;\u0026#x5897;\u0026#x5E45;\u0026#x53EF;\u0026#x80FD;\u0026#x306A;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x306E;\u0026#x92F3;\u0026#x578B;\u0026#x91CF;\u0026#x304C;\u0026#x4F55;\u0026#x500D;\u0026#x306B;\u0026#x5909;\u0026#x5316;\u0026#x3059;\u0026#x308B;\u0026#x304B;\u0026#xFF08;\u0026#x5B9F;\u0026#x9A13;\u0026#x64CD;\u0026#x4F5C;\u0026#x306B;\u0026#x4F34;\u0026#x3046;\u0026#x4E0D;\u0026#x968F;\u0026#x610F;\u0026#x306A;\u0026#x5909;\u0026#x5316;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#xFF09;\u0026#x3002;\u0026#x5143;\u0026#x8AD6;\u0026#x6587;\u0026#x3067; \u0026#x3B4;_B \u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x8868;\u0026#x3055;\u0026#x308C;\u0026#x3066;\u0026#x3044;\u0026#x308B;\u0026#x5909;\u0026#x6570;\u0026#x3002;\u003c/p\u003e\n\u003c/li\u003e\n\u003cli\u003e\n\u003cp\u003eMaeoka et al. (2020) \u0026#x306E;\u0026#x65B9;\u0026#x6CD5;\u0026#x3084;\u0026#x3001;\u0026#x305D;\u0026#x306E;\u0026#x4ED6;\u0026#x306E;\u0026#x3088;\u0026#x304F;\u0026#x3042;\u0026#x308B; \u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3067;\u0026#x306F;\u0026#x3001;\u0026#x3053;\u0026#x306E;\u0026#x5909;\u0026#x6570;\u0026#x306F;\u0026#x5B58;\u0026#x5728;\u0026#x3057;\u0026#x306A;\u0026#x3044;\u0026#xFF08;\u0026#x203B;\u0026#xFF09;\u0026#x3002;\u0026#x3044;\u0026#x305A;\u0026#x308C;\u0026#x306E;\u0026#x5834;\u0026#x5408;\u0026#x3067;\u0026#x3082; \u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x306E;\u0026#x5B9F;\u0026#x884C;\u0026#x306B;\u0026#x306F;\u0026#x4E0D;\u0026#x8981;\u0026#x3067;\u0026#x3042;\u0026#x308A;\u0026#x3001;\u0026#x5F15;\u0026#x6570;\u0026#x306B;\u0026#x542B;\u0026#x307E;\u0026#x308C;\u0026#x306A;\u0026#x3044;\u0026#xFF08;\u0026#x306A;\u0026#x305C;\u0026#x4E0D;\u0026#x8981;\u0026#x304B;\u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x3068;\u0026#x3001;\u003ccode\u003efreqpcr()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x306F; 4 \u0026#x3064;\u0026#x306E; Cq \u0026#x5024;\u0026#x3092; 2 \u0026#x3064;\u0026#x306E; \u0026#x394;Cq \u0026#x306B;\u0026#x63DB;\u0026#x7B97;\u0026#x3057;\u0026#x3066;\u0026#x304B;\u0026#x3089;\u0026#x5C24;\u0026#x5EA6;\u0026#x95A2;\u0026#x6570;\u0026#x306B;\u0026#x6295;\u0026#x5165;\u0026#x3059;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x3067;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x8A73;\u0026#x3057;\u0026#x304F;\u0026#x306F;\u003ca href=\"https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13554#men13554-disp-0014\"\u003e\u0026#x8AD6;\u0026#x6587;\u0026#x306E;\u0026#x6570;\u0026#x5F0F; 11\u003c/a\u003e \u0026#x3092;\u0026#x898B;\u0026#x3066;\u0026#x307B;\u0026#x3057;\u0026#x3044;\u0026#xFF09;\u0026#x3002;\u003c/p\u003e\n\u003c/li\u003e\n\u003c/ul\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u0026#x203B;\u0026#x3053;\u0026#x308C;\u0026#x306F;\u0026#x3001;RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x4EE5;\u0026#x5916;\u0026#x306E;\u0026#x3042;\u0026#x3089;\u0026#x3086;\u0026#x308B; \u0026#x394;\u0026#x394;Cq \u0026#x5206;\u0026#x6790;\u0026#x306B;\u0026#x304A;\u0026#x3044;\u0026#x3066; baseChange \u0026#x304C;\u0026#x5B58;\u0026#x5728;\u0026#x3057;\u0026#x306A;\u0026#x3044;\u0026#x3068;\u0026#x3044;\u0026#x3046;\u0026#x610F;\u0026#x5473;\u0026#x3067;\u0026#x306F;\u0026#x306A;\u0026#x3044;\u0026#x3002;\u0026#x5236;\u0026#x9650;\u0026#x9175;\u0026#x7D20;\u0026#x51E6;\u0026#x7406;\u0026#x3067;\u0026#x306A;\u0026#x304F;\u0026#x3066;\u0026#x3082;\u0026#x3001;\u0026#x5B9F;\u0026#x9A13;\u0026#x6C34;\u0026#x6E96;\u0026#x9593;\u0026#x3067;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x306E;\u0026#x92F3;\u0026#x578B; DNA \u0026#x91CF;\u0026#x306B;\u0026#x3001;\u0026#x4F55;\u0026#x3089;\u0026#x304B;\u0026#x306E;\u0026#x7CFB;\u0026#x7D71;\u0026#x8AA4;\u0026#x5DEE;\u0026#x3092;\u0026#x4E0E;\u0026#x3048;\u0026#x308B;\u0026#x3088;\u0026#x3046;\u0026#x306A;\u0026#x5B9F;\u0026#x9A13;\u0026#x64CD;\u0026#x4F5C;\u0026#x306F;\u0026#x3042;\u0026#x308A;\u0026#x5F97;\u0026#x308B;\u0026#x306E;\u0026#x3067;\u0026#x3001;\u0026#x305D;\u0026#x306E;\u0026#x3088;\u0026#x3046;\u0026#x306A;\u0026#x5B9F;\u0026#x9A13;\u0026#x7CFB;\u0026#x306E;\u0026#x4E2D;\u0026#x306B;\u0026#x306F; RED-\u0026#x394;\u0026#x394;Cq \u0026#x6CD5;\u0026#x3068;\u0026#x540C;\u0026#x69D8;\u0026#x306E;\u0026#x767A;\u0026#x60F3;\u0026#x3067; \u003ccode\u003eknownqpcr()\u003c/code\u003e \u0026#x304A;\u0026#x3088;\u0026#x3073; \u003ccode\u003eknownqpcr_unpaired()\u003c/code\u003e \u0026#x95A2;\u0026#x6570;\u0026#x3092;\u0026#x4F7F;\u0026#x3046;\u0026#x3079;\u0026#x304D;\u0026#x3082;\u0026#x306E;\u0026#x3082;\u0026#x3042;\u0026#x308B;\u0026#x3060;\u0026#x308D;\u0026#x3046;\u0026#x3002;\u003c/p\u003e\n\u003c/blockquote\u003e\n\u003chr\u003e\n\u003ch2 class=\"mume-header\" id=\"knownqpcr-%E3%81%AA%E3%81%84%E3%81%97-knownqpcr_unpaired-%E9%96%A2%E6%95%B0%E3%81%AB%E3%82%88%E3%82%8B%E5%AE%9F%E9%A8%93%E3%83%91%E3%83%A9%E3%83%A1%E3%83%BC%E3%82%BF%E3%81%AE%E6%8E%A8%E5%AE%9A\"\u003eknownqpcr() \u0026#x306A;\u0026#x3044;\u0026#x3057; knownqpcr_unpaired() \u0026#x95A2;\u0026#x6570;\u0026#x306B;\u0026#x3088;\u0026#x308B;\u0026#x5B9F;\u0026#x9A13;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x306E;\u0026#x63A8;\u0026#x5B9A;\u003c/h2\u003e\n\n\u003cp\u003e\u003ca href=\"https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13554#men13554-sec-0010-title\"\u003e\u0026#x539F;\u0026#x8457;\u0026#x8AD6;\u0026#x6587;\u0026#x306E;\u0026#x5B9F;\u0026#x9A13; 1\u003c/a\u003e \u0026#x306B;\u0026#x76F8;\u0026#x5F53;\u0026#x3059;\u0026#x308B;\u0026#x3002;\n\u0026#x7528;\u0026#x3044;\u0026#x308B;\u0026#x30B5;\u0026#x30FC;\u0026#x30DE;\u0026#x30EB;\u0026#x30B5;\u0026#x30A4;\u0026#x30AF;\u0026#x30E9;\u0026#x30FC;\u0026#x3084;\u0026#x5B9F;\u0026#x9A13;\u0026#x30D7;\u0026#x30ED;\u0026#x30C8;\u0026#x30B3;\u0026#x30EB;\u0026#x3054;\u0026#x3068;\u0026#x306B;\u0026#x30D1;\u0026#x30E9;\u0026#x30E1;\u0026#x30FC;\u0026#x30BF;\u0026#x306E;\u0026#x5024;\u0026#x306F;\u0026#x5909;\u0026#x308F;\u0026#x308B;\u0026#x305F;\u0026#x3081;\u0026#x3001;\u0026#x540C;\u0026#x3058;\u0026#x751F;\u0026#x7269;\u0026#x306E;\u0026#x540C;\u0026#x3058;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3092;\u0026#x691C;\u0026#x67FB;\u0026#x3059;\u0026#x308B;\u0026#x5834;\u0026#x5408;\u0026#x3082;\u0026#x5404;\u0026#x30E9;\u0026#x30DC;\u0026#x3067;\u0026#x4E88;\u0026#x3081;\u0026#x8ABF;\u0026#x3079;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x5FC5;\u0026#x8981;\u0026#x304C;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x307E;\u0026#x305A;\u0026#x300C;\u0026#x53B3;\u0026#x5BC6;\u0026#x306B;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#x306E;\u0026#x6DF7;\u0026#x5408;\u0026#x6BD4;\u0026#x304C;\u0026#x5206;\u0026#x304B;\u0026#x3063;\u0026#x3066;\u0026#x3044;\u0026#x308B; DNA \u0026#x6EB6;\u0026#x6DB2;\u0026#x300D;\u0026#x3092;\u0026#x8907;\u0026#x6570;\u0026#x306E;\u0026#x6DF7;\u0026#x5408;\u0026#x6BD4;\u0026#x3067;\u0026#x7528;\u0026#x610F;\u0026#x3059;\u0026#x308B;\u0026#x3002;\u0026#x5B9A;\u0026#x91CF; PCR \u0026#x306E;\u0026#x4E00;\u0026#x822C;\u0026#x7684;\u0026#x306A;\u0026#x30D7;\u0026#x30ED;\u0026#x30C8;\u0026#x30B3;\u0026#x30EB;\u0026#x306B;\u0026#x5F93;\u0026#x3044;\u0026#x3001;\u0026#x691C;\u0026#x51FA;\u0026#x3057;\u0026#x305F;\u0026#x3044;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#xFF08;R\u0026#xFF09;\u0026#x3092;\u0026#x3082;\u0026#x3046; 1 \u0026#x3064;\u0026#x306E;\u0026#x30A2;\u0026#x30EA;\u0026#x30EB;\u0026#xFF08;S\u0026#xFF09;\u0026#x3067;\u0026#x3001;\u0026#x60F3;\u0026#x5B9A;\u0026#x30EC;\u0026#x30F3;\u0026#x30B8;\u0026#x306E;\u0026#x4E0A;\u0026#x9650;\u0026#x304B;\u0026#x3089;\u0026#x4E0B;\u0026#x9650;\u0026#x306B;\u0026#x5411;\u0026#x3051;\u0026#x3066;\u0026#x6BB5;\u0026#x968E;\u0026#x5E0C;\u0026#x91C8;\u0026#x3057;\u0026#x305F;\u0026#x7CFB;\u0026#x5217;\u0026#x3092;\u0026#x4F5C;\u0026#x308B;\u0026#x3068;\u0026#x826F;\u0026#x3044;\u0026#x3002;\u0026#x306A;\u0026#x304A; R \u0026#x983B;\u0026#x5EA6; Y_R = 0 \u0026#x304A;\u0026#x3088;\u0026#x3073; Y_R = 1 \u0026#x306E;\u0026#x7D44;\u0026#x307F;\u0026#x5408;\u0026#x308F;\u0026#x305B;\u0026#x3092;\u0026#x542B;\u0026#x3081;\u0026#x3066;\u0026#x304A;\u0026#x304F;\u0026#x3068;\u0026#x3001;\u0026#x63A8;\u0026#x5B9A;\u0026#x7D50;\u0026#x679C;\u0026#x304C;\u0026#x5B89;\u0026#x5B9A;\u0026#x3057;\u0026#x3084;\u0026#x3059;\u0026#x3044;\u0026#x3002;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026#x6CE8;\u0026#xFF1A;\u0026#x3053;\u0026#x3053;\u0026#x3067;\u0026#x4F7F;\u0026#x3046;\u0026#x6DF7;\u0026#x5408; DNA \u0026#x6EB6;\u0026#x6DB2;\u0026#x306F;\u0026#x672C;\u0026#x756A;\u0026#x306E;\u0026#x30B5;\u0026#x30F3;\u0026#x30D7;\u0026#x30EB;\u0026#x3068;\u0026#x540C;\u0026#x69D8;\u0026#x3001;\u0026#x30BF;\u0026#x30FC;\u0026#x30B2;\u0026#x30C3;\u0026#x30C8;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3060;\u0026#x3051;\u0026#x3067;\u0026#x306A;\u0026#x304F;\u0026#x30CF;\u0026#x30A6;\u0026#x30B9;\u0026#x30AD;\u0026#x30FC;\u0026#x30D4;\u0026#x30F3;\u0026#x30B0;\u0026#x907A;\u0026#x4F1D;\u0026#x5B50;\u0026#x3092;\u0026#x542B;\u0026#x3093;\u0026#x3067;\u0026#x3044;\u0026#x308B;\u0026#x5FC5;\u0026#x8981;\u0026#x304C;\u0026#x3042;\u0026#x308B;\u0026#x3002;\u0026#x78BA;\u0026#x5B9F;\u0026#x306A;\u0026#x65B9;\u0026#x6CD5;\u0026#x3068;\u0026#x3057;\u0026#x3066;\u0026#x306F;\u0026#x305F;\u0026#x3068;\u0026#x3048;\u0026#x3070; R \u0026#x7CFB;\u0026#x7D71;\u0026#x3068; S \u0026#x7CFB;\u0026#x7D71;\u0026#x306E;\u0026#x5404;","project_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fsudoms%2Ffreqpcr","html_url":"https://awesome.ecosyste.ms/projects/github.com%2Fsudoms%2Ffreqpcr","lists_url":"https://awesome.ecosyste.ms/api/v1/projects/github.com%2Fsudoms%2Ffreqpcr/lists"}