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https://github.com/nf-core/modules

Repository to host tool-specific module files for the Nextflow DSL2 community!
https://github.com/nf-core/modules

dsl2 modules nextflow nf-core nf-test pipelines workflows

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Repository to host tool-specific module files for the Nextflow DSL2 community!

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README

        

# ![nf-core/modules](docs/images/nfcore-modules_logo.png)

[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)
[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)

![GitHub Actions Coda Linting](https://github.com/nf-core/modules/workflows/Code%20Linting/badge.svg)
[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23modules-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/modules)

[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)
[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)

> THIS REPOSITORY IS UNDER ACTIVE DEVELOPMENT. SYNTAX, ORGANISATION AND LAYOUT MAY CHANGE WITHOUT NOTICE!

A repository for hosting [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) module files containing tool-specific process definitions and their associated documentation.

## Table of contents

- [Using existing modules](#using-existing-modules)
- [Adding new modules](#adding-new-modules)
- [Help](#help)
- [Citation](#citation)

## Using existing modules

The module files hosted in this repository define a set of processes for software tools such as `fastqc`, `bwa`, `samtools` etc. This allows you to share and add common functionality across multiple pipelines in a modular fashion.

We have written a helper command in the `nf-core/tools` package that uses the GitHub API to obtain the relevant information for the module files present in the [`modules/`](modules/) directory of this repository. This includes using `git` commit hashes to track changes for reproducibility purposes, and to download and install all of the relevant module files.

1. Install the latest version of [`nf-core/tools`](https://github.com/nf-core/tools#installation) (`>=2.0`)
2. List the available modules:

```console
$ nf-core modules list remote

,--./,-.
___ __ __ __ ___ /,-._.--~\
|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'

nf-core/tools version 2.0

INFO Modules available from nf-core/modules (master): pipeline_modules.py:164

┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┓
┃ Module Name ┃
┡━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┩
│ bandage/image │
│ bcftools/consensus │
│ bcftools/filter │
│ bcftools/isec │
..truncated..
```

3. Install the module in your pipeline directory:

```console
$ nf-core modules install fastqc

,--./,-.
___ __ __ __ ___ /,-._.--~\
|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'

nf-core/tools version 2.0

INFO Installing fastqc pipeline_modules.py:213
INFO Downloaded 3 files to ./modules/nf-core/modules/fastqc pipeline_modules.py:236
```

4. Import the module in your Nextflow script:

```nextflow
#!/usr/bin/env nextflow

nextflow.enable.dsl = 2

include { FASTQC } from './modules/nf-core/modules/fastqc/main'
```

5. Remove the module from the pipeline repository if required:

```console
$ nf-core modules remove fastqc

,--./,-.
___ __ __ __ ___ /,-._.--~\
|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'

nf-core/tools version 2.0

INFO Removing fastqc pipeline_modules.py:271
INFO Successfully removed fastqc pipeline_modules.py:285
```

6. Check that a locally installed nf-core module is up-to-date compared to the one hosted in this repo:

```console
$ nf-core modules lint fastqc

,--./,-.
___ __ __ __ ___ /,-._.--~\
|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'

nf-core/tools version 2.0

INFO Linting pipeline: . lint.py:104
INFO Linting module: fastqc lint.py:106

╭─────────────────────────────────────────────────────────────────────────────────╮
│ [!] 1 Test Warning │
╰─────────────────────────────────────────────────────────────────────────────────╯
╭──────────────┬───────────────────────────────┬──────────────────────────────────╮
│ Module name │ Test message │ File path │
├──────────────┼───────────────────────────────┼──────────────────────────────────┤
│ fastqc │ Local copy of module outdated │ modules/nf-core/modules/fastqc/ │
╰──────────────┴────────────────────────────── ┴──────────────────────────────────╯
╭──────────────────────╮
│ LINT RESULTS SUMMARY │
├──────────────────────┤
│ [✔] 15 Tests Passed │
│ [!] 1 Test Warning │
│ [✗] 0 Test Failed │
╰──────────────────────╯
```

## Adding new modules

If you wish to contribute a new module, please see the documentation on the [nf-core website](https://nf-co.re/developers/modules#writing-a-new-module-reference).

> Please be kind to our code reviewers and submit one pull request per module :)

## Help

For further information or help, don't hesitate to get in touch on [Slack `#modules` channel](https://nfcore.slack.com/channels/modules) (you can join with [this invite](https://nf-co.re/join/slack)).

## Citation

If you use the module files in this repository for your analysis please you can cite the `nf-core` publication as follows:

> **The nf-core framework for community-curated bioinformatics pipelines.**
>
> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.
>
> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).