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https://github.com/AstraZeneca/skywalkR

code for Gogleva et al manuscript
https://github.com/AstraZeneca/skywalkR

drug-discovery knowledge-graph recommender-system shiny-apps ui

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code for Gogleva et al manuscript

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README 

        
![Maturity level-Prototype](https://img.shields.io/badge/Maturity%20Level-Prototype-red) [![DOI](https://zenodo.org/badge/371064106.svg)](https://zenodo.org/badge/latestdoi/371064106)



## skywalkR



This repository contains code and documentation to accompany Gogleva et al manuscript: **Knowledge Graph-based Recommendation Framework Identifies Novel Drivers of Resistance in EGFR mutant Non-small Cell Lung Cancer**. 



To read the paper please follow: [https://www.biorxiv.org/content/10.1101/2021.07.23.453506v1.full.pdf+html](https://www.nature.com/articles/s41467-022-29292-7)



The repository has the following structure:



```

.

├── DESCRIPTION

├── CONTRIBUTING.md

├── LICENSE

├── 🛠️ R --> helper functions for shiny apps

│   ├── server_utils.R

│   └── ui_utils.R

├── README.md

├── renv.lock

├── 💾 data --> necessary input data and sample data files

│   ├── app_data.csv

│   ├── heatmap_data.tsv

│   ├── mtcars.csv

│   ├── nlp_allowed.csv

│   └── sample_data.csv

├── 📄 docs --> within the app documentation

│   ├── intro.md

│   ├── intro_light.md

│   ├── pareto_bar_explained.md

│   ├── pareto_hist_explained.md

│   └── variables_explained.md

├── 🖼️ img --> readme plots

│   ├── skywalkR_interface.png

│   └── skywalkr_light_interface.png

├── 🧬 skywalkR_app.R --> main Shiny app

re-rank CRISPR hits to find promising genes driving drug

resistance in EGFR mutant lung cancer;

└── 🧪 skywalkR_light_app.R --> a demo shiny app,

input any table to test out how multi-objective optimisation works;

```



### SkywalkR app



This Shiny app is tailored to finding genes driving resistance in EGFR mutant Non-small Cell Line Cancer (NSCLC). The app will automatically load required data. To re-rank genes set directions of optimization by moving sliders and press ``rank!`` button.



![skywalkr_app](https://github.com/AstraZeneca/skywalkR/blob/master/img/skywalkR_interface.png)



### SkywalkR_light app



This is a generic app, bare bones app that allows users to apply the same idea of multi-objective optimization to their own data. Any tabular numeric data should work. To include categorical data, please represent it as numeric. By default we will treat the first column as labels. Columns will be automatically mapped to sliders.



![skywalkr_light_app](https://github.com/AstraZeneca/skywalkR/blob/master/img/skywalkR_light_interface.png)



### Running apps



To install all the packages required to run the apps, call ``renv::restore()`` as declared in the lockfile. Typically, it can take a few minutes to install the dependencies, depending on which ones are already installed in your environment.



After installing run the respective app, it should start immediately:



``R -e "shiny::runApp('skywalkR_light_app.R')"``



or



``R -e "shiny::runApp('skywalkR_app.R')"``



Typical start time for the app 10-20 seconds. Time estimates are provided for MacBook Pro, 6-Core Intel Core i7, 2.6 GHz, 6 cores, 32 GB, Big Sur.



### Citation 

```

@Article{Gogleva2022,

author={Gogleva, Anna

and Polychronopoulos, Dimitris

and Pfeifer, Matthias

and Poroshin, Vladimir

and Ughetto, Micha{\"e}l

and Martin, Matthew J.

and Thorpe, Hannah

and Bornot, Aurelie

and Smith, Paul D.

and Sidders, Ben

and Dry, Jonathan R.

and Ahdesm{\"a}ki, Miika

and McDermott, Ultan

and Papa, Eliseo

and Bulusu, Krishna C.},

title={Knowledge graph-based recommendation framework identifies drivers of resistance in EGFR mutant non-small cell lung cancer},

journal={Nature Communications},

year={2022},

month={Mar},

day={29},

volume={13},

number={1},

pages={1667},

abstract={Resistance to EGFR inhibitors (EGFRi) presents a major obstacle in treating non-small cell lung cancer (NSCLC). One of the most exciting new ways to find potential resistance markers involves running functional genetic screens, such as CRISPR, followed by manual triage of significantly enriched genes. This triage process to identify `high value' hits resulting from the CRISPR screen involves manual curation that requires specialized knowledge and can take even experts several months to comprehensively complete. To find key drivers of resistance faster we build a recommendation system on top of a heterogeneous biomedical knowledge graph integrating pre-clinical, clinical, and literature evidence. The recommender system ranks genes based on trade-offs between diverse types of evidence linking them to potential mechanisms of EGFRi resistance. This unbiased approach identifies 57 resistance markers from >3,000 genes, reducing hit identification time from months to minutes. In addition to reproducing known resistance markers, our method identifies previously unexplored resistance mechanisms that we prospectively validate.},

issn={2041-1723},

doi={10.1038/s41467-022-29292-7},

url={https://doi.org/10.1038/s41467-022-29292-7}

}

```