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https://github.com/Bioconductor/LearnBioconductor

Training material for introductory R / Bioconductor courses
https://github.com/Bioconductor/LearnBioconductor

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Training material for introductory R / Bioconductor courses

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README

        

Learning R / Bioconductor for Sequence Analysis
===============================================

Fred Hutchinson Cancer Research Center, Seattle, WA

October 27-29

Location: October 27 / 28: Arnold Building M1-A303; October 29:
Thomas D1-080

Contact: Martin Morgan (content,
[[email protected]](mailto:[email protected])); Melissa Alvendia
(administration, [[email protected]](mailto:[email protected]))

This course is directed at beginning and intermediate users who would
like an introduction to the analysis and comprehension of
high-throughput sequence data using _R_ and
_[Bioconductor](http://bioconductor.org)_. Day 1 focuses on learning
essential background: an introduction to the _R_ programming language;
central concepts for effective use of _Bioconductor_ software; and an
overview of high-throughput sequence analysis work flows. Day 2
emphasizes use of _Bioconductor_ for specific tasks: an RNA-seq
differential expression work flow; exploratory, machine learning, and
other statistical tasks; gene set enrichment; and annotation. Day 3
transitions to understanding effective approaches for managing larger
challenges: strategies for working with large data, writing re-usable
functions, developing reproducible reports and work flows, and
visualizing results. The course combines lectures with extensive
hands-on practicals; students are required to bring a laptop with
wireless internet access and a modern version of the Chrome or Safari
web browser.

Schedule (tentative)
--------------------

Day 1: Learn _R_ / _Bioconductor_

- 9:00 - 10:30 Introduction to _R_: objects, functions, help!
- 11:00 - 12:30 Introduction to _Bioconductor_: working with packages and classes
- 1:30 - 5:00 (break: 3:00 - 3:30) Introduction to sequence analysis:
typical work flow; data types and quality assessment; essential
_Bioconductor_ packages

Day 2: Use _R_ / _Bioconductor_

- 9:00 - 12:30 (break (10:30 - 11:00) An RNA-seq differential
expression work flow (detail)
- 1:30 - 2:00 Other work flows (survey): ChIP-seq, variants, copy
number, epigenomics
- 2:00 - 3:00 Machine learning; exploratory and other statistical
analysis
- 3:30 - 4:00 Annotating genes, genomes, and variants
- 4:00 - 5:00 Approaches to gene set enrichment

Day 3: Develop Skills and Best Practices

- 9:00 - 10:30 Working with large data
- 11:00 - 12:30 Organizing code in functions, files, and packages
- 1:30 - 3:00 Reproducible reports and work flows: markdown
- 3:30 - 4:30 Visualization
- 4:30 - 5:00 Summary