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https://github.com/Bioconductor/UseBioconductor

Training material for intermediate R / Bioconductor courses
https://github.com/Bioconductor/UseBioconductor

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Training material for intermediate R / Bioconductor courses

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README

        

Use R / Bioconductor for Sequence Analysis
==========================================

Fred Hutchinson Cancer Research Center, Seattle, WA

6-7 April, 2015

Contact: Martin Morgan
([[email protected]](mailto:[email protected]))

This **INTERMEDIATE** course is designed for individuals comfortable
using _R_, and with some familiarity with _Bioconductor_. It consists
of approximately equal parts lecture and practical sessions addressing
use of _Bioconductor_ software for analysis and comprehension of
high-throughput sequence and related data. Specific topics include use
of central Bioconductor classes (e.g., _GRanges_,
_SummarizedExperiment_), RNASeq gene differential expression, ChIP-seq
and methylation work flows, approaches to management and integrative
analysis of diverse high-throughput data types, and strategies for
working with large data. Participants are required to bring a laptop
with wireless internet access and a modern version of the Chrome or
Safari web browser.

Registration
------------

Please register [online](https://register.bioconductor.org/Seattle-Apr-2015/).

Schedule (tentative)
--------------------

Day 1 (9:00 - 12:30; 1:30 - 5:00)

- [A. Introduction](vignettes/A_Introduction.Rmd). _Bioconductor_ and
sequencing work flows
- [B. Genomic Ranges](vignettes/B_GenomicRanges.Rmd). Working with Genomic
Ranges and other _Bioconductor_ data structures (e.g., in the
[GenomicRanges](http://bioconductor.org/packages/devel/bioc/html/GenomicRanges.html).
package).
- [C. Differential Gene Expression](vignettes/C_DifferentialExpression.Rmd). RNA-Seq
known gene differential expression with
[DESeq2](http://bioconductor.org/packages/devel/bioc/html/DESeq2.html)
and
[edgeR](http://bioconductor.org/packages/devel/bioc/html/edgeR.html).

Day 2 (9:00 - 12:30; 1:30 - 5:00)

- [D. Machine Learning](vignettes/D_MachineLearning.Rmd).
- [E. Gene Set Enrichment](vignettes/E_GeneSetEnrichment.Rmd).
- [F. ChIP-seq](vignettes/F_ChIPSeq.Rmd) ChIP-seq with
[csaw](http://bioconductor.org/packages/devel/bioc/html/csaw.html)
- [I. Large Data](vignettes/I_LargeData.Rmd) -- efficient, parallel, and cloud
programming with
[BiocParallel](http://bioconductor.org/packages/devel/bioc/html/BiocParallel.html),
[GenomicFiles](http://bioconductor.org/packages/devel/bioc/html/GenomicFiles.html),
and other resources.