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https://github.com/JoachimGoedhart/VolcaNoseR

Shiny app for making and annotating Volcano plots
https://github.com/JoachimGoedhart/VolcaNoseR

dataviz genomic-data-analysis ggplot2 proteomics-data-analysis shiny shinyapp volcano

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Shiny app for making and annotating Volcano plots

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README

        

# VolcaNoseR
A Shiny App for making Volcano plots

### Running the App

The web-tool runs from a shiny server, and can be accessed at:

o [https://huygens.science.uva.nl/VolcaNoseR/](https://huygens.science.uva.nl/VolcaNoseR/)

o [https://huygens.science.uva.nl/VolcaNoseR2/](https://huygens.science.uva.nl/VolcaNoseR2/)

o [https://amsterdamstudygroup.shinyapps.io/VolcaNoseR/](https://amsterdamstudygroup.shinyapps.io/VolcaNoseR/)

o [https://goedhart.shinyapps.io/VolcaNoseR/](https://goedhart.shinyapps.io/VolcaNoseR/), while bandwidth lasts

Alternatively, the app can run from R/Rstudio and this is perhaps the best option for loading large files. See instructions below

#### Preparations
Note that the app depends on several R packages that need to be installed (shiny, ggplot2, dplyr, magrittr, ggrepel, DT, shinycssloaders, RCurl, readxl).

Run this command in R/Rstudio to download and install all the packages (only needs to be done once):
```
install.packages("shiny", "tidyverse", "ggrepel", "DT", "shinycssloaders", "RCurl", "readxl", "ggiraph", "htmlwidgets")
```
o The first option is running it directly from Github. In the command line (in R or Rstudio) type:
```
shiny::runGitHub('VolcaNoseR', 'JoachimGoedhart')
```
o The second option is download the app and to use it offline:

-download the `app.R` and csv files (`Data-Vulcano-plot.csv` and `elife-45916-Cdc42QL_data.csv`) with example data.

-Run RStudio and load `app.R`

-Select 'Run All' (shortcut is command-option-R on a Mac) or click on "Run App" (upper right button on the window)

This should launch a web browser with the Shiny app.

### Credits

There are several Shiny apps for Volcano plots that have served as a source of inspiration:

-[VolcanoR](https://github.com/vovalive/volcanoR)

-[Volcanoshiny](https://github.com/hardingnj/volcanoshiny)

-[VolcanoPlot_shiny_app](https://github.com/stemicha/VolcanoPlot_shiny_app)

VolcaNoseR is created and maintained by Joachim Goedhart ([@joachimgoedhart](https://twitter.com/joachimgoedhart))

### Example output

Standard output generated with the example data:

![alt text](https://github.com/JoachimGoedhart/VolcaNoseR/blob/master/VolcaNoseR_example1.png "Output")

Output with user selected annotation of data:

![alt text](https://github.com/JoachimGoedhart/VolcaNoseR/blob/master/VolcaNoseR_example2.png "Output")