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https://github.com/bmascat/lfq-proteomics-pipeline

All code, bash scrypts and pipelines used to analyse and visualise proteomic data from my experiments with my first Crispr KO model.
https://github.com/bmascat/lfq-proteomics-pipeline

bioinformatics goterm over-representation-analysis proteomics proteomics-data-analysis

Last synced: 28 days ago
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All code, bash scrypts and pipelines used to analyse and visualise proteomic data from my experiments with my first Crispr KO model.

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# Proteomic analysis of CRISPR Hela KO Alms1 after TGF-B stimulation

All code and pipelines used to analyze and visualize proteomic data from my experiments with my first Crispr KO model


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Differenticial expression analysis was performed with DEP package:

>Zhang X, Smits A, van Tilburg G, Ovaa H, Huber W, Vermeulen M (2018). “Proteome-wide identification of ubiquitin interactions using UbIA-MS.” Nature Protocols, 13, 530–550.

Go-Terms analysis and data results visualizations was performed with Clusterprofiler and GOplot packages to R:

>Yu G, Wang L, Han Y, He Q (2012). “clusterProfiler: an R package for comparing biological themes among gene clusters.” OMICS: A Journal of Integrative Biology, 16(5), 284-287. doi: 10.1089/omi.2011.0118.

>Walter, Wencke, Fátima Sánchez-Cabo, and Mercedes Ricote. "GOplot: an R package for visually combining expression data with functional analysis." Bioinformatics (2015): btv300.