https://github.com/chrisnelsonlab/diy-genome-2021
https://github.com/chrisnelsonlab/diy-genome-2021
Last synced: 3 months ago
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- Host: GitHub
- URL: https://github.com/chrisnelsonlab/diy-genome-2021
- Owner: chrisnelsonlab
- Created: 2021-06-23T18:15:55.000Z (almost 4 years ago)
- Default Branch: main
- Last Pushed: 2021-07-16T11:14:30.000Z (almost 4 years ago)
- Last Synced: 2025-01-17T01:49:21.839Z (5 months ago)
- Size: 423 KB
- Stars: 0
- Watchers: 1
- Forks: 0
- Open Issues: 0
-
Metadata Files:
- Readme: README.md
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README
# DIY-Genome-2021
## Overview
Today we will examine the topic of CRISPR. It is our hope that you by going through the day, you will:
* Learn what CRISPR can do
* Understand where CRISPR came from
* Gain the confidence to perform a CRISPR edit yourself
* Discuss what is an ethical and unethical use of CRISPR
We will be available to answer questions and discuss throughout the day. We wish this could be in person so you could do a gene edit of your own!
## Schedule and Links
## 9:00 - Pre class survey and meet on Teams
Take this survey which will help us improve this course for next year!
https://forms.gle/wNdMFgJBy5Ht28Lo9
Please use our class microsoft teams link when you are done with the survey!
## 9:10 – 10:00 Welcome and Motivation - Live lecture
Please use our class microsoft teams link
## 10:00 – 11:30 - DNA Sequence Analysis + Python
We will be on the microsoft teams link if you have questions or get stuck!
Our Google colab notebook is accessible here:
https://colab.research.google.com/drive/1afweuVhFsarVHJT-e1wC-KXwu0SfplVn?usp=sharing
* 1.1 Python introduction/review
* 1.2 DNA and the Central Dogma of Biology
* 1.3 Strings and DNA
* 1.4 DNA Alignment (with advanced topics)
* 1.5 The human genome project and DNA Databases
* Challenge #1
## 11:30 – 12:30 Lunch Break and Microsoft Teams Q&A
Please use our class microsoft teams link
## 12:30 – 1:30 - DNA Damage and Repair
https://colab.research.google.com/drive/1od4vSYSv4-LWPk7NDoQH5VMc66bfpLpZ?usp=sharing
* 2.1 DNA Damage
* 2.2 DNA Mutations and genetic disease
* 2.3 Gene Therapy
* 2.4 Breaking DNA to fix DNA
## 1:30 – 2:30 - CRISPR, Immunity, and Evolution
https://colab.research.google.com/drive/1X3pTfCQONvxLAqCzvaypvRzh7NBQG5oc?usp=sharing
* 3.1 CRISPR Repeats
* 3.2 Adaptive Immunity
* Challenge #2
* 3.3 CRISPR for biotechnology
* 3.4 Make your own CRISPR edits!
* Challenge #3
## 2:30 – 3:30 - Applications and Ethics of Bioengineering with CRISPR
https://colab.research.google.com/drive/169TAfOqSEQZO9q4qkcgstpVisEUhY3yG?usp=sharing
* 4.1 Challenges for gene therapies
* 4.2 Applications for genetic disease
* 4.3 Applications for Cancer
* 4.4 Applications for diagnostics
* 4.5 Ethics
## 3:30 – 4:00 Wrap up discussion and Q&A.
The last thing we will do is meet to discuss the applications and ethics of CRISPR as a group. We will answer poll questions about ethical considerations and discuss future applications of gene editing.
If you have any questions that come up after the class, feel free to contact me at [email protected]. Thank you for participating in the class. I hope you enjoyed it.
If you have time, this post class survey is short and will help us improve the class for next year!
https://forms.gle/HVDekSqmLgXHBgv68