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https://github.com/complextissue/pyclustree

A Python alternative to `clustree` for assessing single-cell RNA-sequencing clusters.
https://github.com/complextissue/pyclustree

clustree pyclustree scverse single-cell

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A Python alternative to `clustree` for assessing single-cell RNA-sequencing clusters.

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# pyclustree

[![Version](https://img.shields.io/pypi/v/pyclustree)](https://pypi.org/project/pyclustree/)
[![License](https://img.shields.io/pypi/l/pyclustree)](https://img.shields.io/pypi/l/pyclustree)
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[![Python Version Required](https://img.shields.io/pypi/pyversions/pyclustree)](https://pypi.org/project/pyclustree/)
[![DOI](https://zenodo.org/badge/857752929.svg)](https://doi.org/10.5281/zenodo.13987570)

Visualize cluster assignments at different resolutions. Possbile applications include finding the optimal resolution for
single-cell RNA-sequencing clusterings.

`pyclustree` is inspired by the R package `clustree` ([Github repository](https://github.com/lazappi/clustree)), however, while we aim to provide the same functionality, the API
will differ between the implementations.

## Getting started

Please refer to the [documentation][link-docs].

## Installation

You need to have Python 3.10 or newer installed on your system. If you don't have
Python installed, we recommend installing [Mambaforge](https://github.com/conda-forge/miniforge#mambaforge).

There are several alternative options to install pyclustree:

1. Install the latest release of `pyclustree` from [PyPI][link-pypi]:

```bash
pip install pyclustree
```

2. Install the latest development version:

```bash
pip install git+https://github.com/complextissue/pyclustree.git@dev
```

## Contact

If you found a bug, please use the [issue tracker][issue-tracker].

## Authors

@maltekuehl
@harryhaller001

Unaffiliated with the creators of the R package `clustree`.

## License

Please refer to the [LICENSE][license] file.

## Citation

Please cite both the original R package as well as this implementation when using `pyclustree`. For example: Cluster resolution was determined based on visualization with pyclustree (Kuehl et al., 2024), a Python implementation of clustree (Zappia et al., 2018).

- pyclustree: Kuehl, M., Hellmig, M., & Puelles, V. G. (2024). pyclustree: Visualizing cluster resolution optimization for biomedical data (0.3.1). Zenodo. https://doi.org/10.5281/zenodo.13987570
- clustree: Zappia, L., & Oshlack, A. (2018). Clustering trees: a visualization for evaluating clusterings at multiple resolutions. Gigascience, 7(7), giy083.

[license]: https://github.com/complextissue/pyclustree/blob/main/LICENSE
[issue-tracker]: https://github.com/complextissue/pyclustree/issues
[changelog]: https://pyclustree.readthedocs.io/latest/changelog.html
[link-docs]: https://pyclustree.readthedocs.io
[link-pypi]: https://pypi.org/project/pyclustree