https://github.com/crisprverse/tutorials
Tutorials for the crisprVerse
https://github.com/crisprverse/tutorials
bowtie cas12a cas13 crispr crispr-analysis crispr-cas crispr-design crispr-target genome-analysis grna grna-sequence grna-sequences
Last synced: 4 months ago
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Tutorials for the crisprVerse
- Host: GitHub
- URL: https://github.com/crisprverse/tutorials
- Owner: crisprVerse
- License: mit
- Created: 2022-07-08T18:23:39.000Z (almost 4 years ago)
- Default Branch: master
- Last Pushed: 2025-02-10T18:15:56.000Z (over 1 year ago)
- Last Synced: 2025-02-10T19:28:36.251Z (over 1 year ago)
- Topics: bowtie, cas12a, cas13, crispr, crispr-analysis, crispr-cas, crispr-design, crispr-target, genome-analysis, grna, grna-sequence, grna-sequences
- Language: TeX
- Homepage:
- Size: 5.12 MB
- Stars: 10
- Watchers: 1
- Forks: 0
- Open Issues: 2
-
Metadata Files:
- Readme: README.md
- License: LICENSE
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README
# Tutorials for the crisprVerse
### Getting started
1. [Installation](https://github.com/crisprVerse/Tutorials/tree/master/Installation)
2. [Building a genome index for off-target alignment](https://github.com/crisprVerse/Tutorials/tree/master/Building_Genome_Indices)
3. [Specifying a custom nuclease](https://github.com/crisprVerse/Tutorials/tree/master/Building_Custom_Nuclease)
4. [Building a gene annotation object](https://github.com/crisprVerse/Tutorials/tree/master/Building_Gene_Annotation)
### Common gRNA design workflows
5. [CRISPR knockout (CRISPRko) design with Cas9](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRko_Cas9)
6. [CRISPR knockout (CRISPRko) design with Cas12a](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRko_Cas12a)
7. [CRISPR knockdown (CRISPRkd) design with Cas13d](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRkd_CasRx)
8. [CRISPR knockdown (CRISPRkd) design with the Csm complex](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRkd_Csm)
9. [CRISPR base editing (CRISPRbe) design with BE4max](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRbe)
10. [CRISPR activation (CRISPRa) design](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRa)
11. [CRISPR interference (CRISPRi) design](https://github.com/crisprVerse/Tutorials/tree/master/Design_CRISPRi)
### Less common gRNA design workflows
12. [Paired gRNA design](https://github.com/crisprVerse/Tutorials/tree/master/Design_PairedGuides)
13. [Working with custom DNA sequences](https://github.com/crisprVerse/Tutorials/tree/master/Design_Custom_Sequence)
14. [Design for optical pooled screening (OPS)](https://github.com/crisprVerse/Tutorials/tree/master/Design_OPS)
### Miscellaneous
15. [Mapping gRNAs across species](https://github.com/crisprVerse/Tutorials/tree/master/Design_Cross_Reactivity)
16. [Working with minor and major alleles](https://github.com/crisprVerse/Tutorials/tree/master/Design_Minor_Major_Allele)
17. [Validating existing gRNA libraries](https://github.com/crisprVerse/Tutorials/tree/master/Validating_Existing_gRNA_Libraries)
18. [Building a gRNA database across all protein-coding genes](https://github.com/crisprVerse/Tutorials/tree/master/Building_Database_Human_Cas9)