https://github.com/emx77/ASI_MTF

Imagej macro to calculate the MTF based on a knife edge measurement
https://github.com/emx77/ASI_MTF

electron-microscopy image-analysis imagej imagej-macro imagej-plugins mtf point-spread-function x-ray-imaging

Last synced: 28 days ago
JSON representation

Imagej macro to calculate the MTF based on a knife edge measurement

• Host: GitHub
• URL: https://github.com/emx77/ASI_MTF
• Owner: emx77
• Created: 2018-03-15T23:52:49.000Z (over 6 years ago)
• Default Branch: master
• Last Pushed: 2023-03-17T07:05:50.000Z (over 1 year ago)
• Last Synced: 2024-08-04T00:12:39.088Z (4 months ago)
• Topics: electron-microscopy, image-analysis, imagej, imagej-macro, imagej-plugins, mtf, point-spread-function, x-ray-imaging
• Language: ImageJ Macro
• Size: 916 KB
• Stars: 14
• Watchers: 2
• Forks: 2
• Open Issues: 0
• Metadata Files:
  • Readme: README.md

Awesome Lists containing this project

• awesome-biological-image-analysis - ASI_MTF - ImageJ macro to calculate the modulation transfer function (MTF) based on a knife edge (or slanted edge) measurement. (Electron and super resolution microscopy)

README

        
# ASI_MTF

Imagej macro (ASI_MTF.ijm) to calculate the modulation transfer function (MTF) based on a knife edge (or slanted edge) measurement.

# Installation

In ImageJ, click on Plugins, Install... and open ASI_MTF.ijm to add this macro to the ImageJ plugin menu.

# Usage

Open an image file of a knife edge measurement. Select a rectangular area around the edge and the macro will calculate

the MTF as a function of pixel spatial frequency. A test image with several edges can be found in the data folder.

The edge does not have to be straight, however the macro needs one edge in the region of interest. The region of interest has to be sufficiently large to have enough data for the calculations.

The MTF will be calculated in two ways. The first one is based on a line spread histogram. The 2nd one is based on a Gaussian fit of the line spread data points for all pixels. Results representing the point spread function (PSF) and edge or line spread function (ESF or LSF) are also available.

ASI_NNPS.ijm is a macro under development to calculate the NNPS based on a stack of flat field (open beam) images. It runs in ImageJ 1.51.

# Author

Erik Maddox (erik.maddoxATamscins.com), Amsterdam Scientific Instruments B.V.

# Applications

J. Salido, ea., "MicroHikari3D: an automated DIY digital microscopy platform with deep learning capabilities", Biomed. Opt. Express 12, 7223-7243 (2021) (https://doi.org/10.1364/BOE.439014)

R. Peng, ea. "Characterizing the resolution and throughput of the Apollo direct electron detector", Journal of Structural Biology: X

Volume 7, 2023, 100080 (https://www.sciencedirect.com/science/article/pii/S2590152422000216)