https://github.com/fabsta/hieranoid

https://github.com/fabsta/hieranoid

Last synced: about 5 hours ago
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• Host: GitHub
• URL: https://github.com/fabsta/hieranoid
• Owner: fabsta
• License: gpl-3.0
• Created: 2012-12-04T12:58:52.000Z (almost 12 years ago)
• Default Branch: master
• Last Pushed: 2013-08-15T21:43:34.000Z (over 11 years ago)
• Last Synced: 2023-10-20T19:47:18.284Z (about 1 year ago)
• Language: Perl
• Size: 14.2 MB
• Stars: 7
• Watchers: 2
• Forks: 5
• Open Issues: 2
• Metadata Files:
  • Readme: README
  • License: COPYING

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README

        
Hieranoid

Hierarchical orthology inference

This software is freely available at https://github.com/fabsta/Hieranoid 

Please read the COPYING file before using this software.  

If you use Hieranoid please cite:

Fabian Schreiber and Erik L.L. Sonnhammer

Hieranoid: Hierarchical orthology inference 

Journal of Molecular Biology (submitted)

Installation

Checking system requirements

-	Perl

-	BioPerl

-	Usearch or Blastall

-	HHSearch

-	Muscle or Kalign

Be advised

Perl, BioPerl, Blastall, Muscle and Kalign can be installed with 'apt-get install' command

HHsearch and Usearch need to be installed manually

Perl modules

Hieranoid uses Perl modules that are installed in the 'lib' sub-directory of the Hieranoid installation.

Please add that directory to your PERL5LIB enviromental variable. In bash you do that the following way:

	export PERL5LIB=$PERL5LIB:hieranoid_directory/lib

	

Be advised 

For cluster users, several Perl modules need to be install manually, possibly including:

Module::Implementation	Module::Runtime

Class:Load	Class:MOP

Sub::Exporter	Sub::Install	Sub::Name

Try::Tiny

Package::DeprecationManager	Package::Stash

Moose

Clone

Data::OptList

Params::Util

Configuring Hieranoid

Once you have downloaded Hieranoid, you have to tell Hieranoid where to find the required programs. 

This is done by adapting the settings in the Configuration file (Configurations/Configuration.pm).

All options should be explained in greater detail in the Configuration file

Starting hieranoid

Once, the configuration file has been adapted, the analysis can be started by typing:

	perl hieranoid.pl

Note that depending on the input size, the analysis can take some time

Command line arguments

Additional options:

	-c configurationFile

	-t guide tree file

Test dataset:

To test the installation, simply type “perl hieranoid.pl”.

This will run a quick analysis on the sequences in data/sequences

using species tree in data/tree.

Program output:

All program output is stored in a directory ‘project1’. For each inner node of the provided species tree, a folder will be created in ‘project1/nodes’.

Those folders contain several files: The input query files (e.g. Homo_sapiens.fa), Blast results (e.g. Homo_sapiens-Pan_troglodytes), the InParanoid output (sqltable.Homininae) and the orthology predictions (e.g. Homininae.OGTree.txt).

Where to put my files:

Hieranoid requires a set of sequences as well as a guide tree. By default the Hieranoid searches for the sequences in data/sequences and for the guide tree

in data/tree. These options can be changed in the Configuration file.

Output:

The following is an example of the Hieranoid output format:

HieranoidOG-Eukaryota3:((At1g50030.1:1.000,((ENSPTRP00000000280:1.000,ENSP00000354558:1.000)Homininae1:1.000,ENSMUSP00000099510:1.000)Euarchontoglires1:1.000)Eukaryota3:1.000)root;

The first is the name of the Hieranoid group followed by a newick string with inparalog scores