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https://github.com/gear-genomics/tracy

Basecalling, alignment, assembly and deconvolution of Sanger Chromatogram trace files
https://github.com/gear-genomics/tracy

alignment chromatogram crispr-analysis crispr-cas9 genetic-engineering indel indel-discovery pcr pcr-products sanger-chromatograms sanger-sequencing sanger-trace-alignment sequencing tracy variant-calling

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Basecalling, alignment, assembly and deconvolution of Sanger Chromatogram trace files

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README

          

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## Tracy: basecalling, alignment, assembly and deconvolution of Sanger Chromatogram trace files

Tracy is available as a [Bioconda package](https://anaconda.org/bioconda/tracy), as a pre-compiled statically linked binary from [Tracy's github release page](https://github.com/gear-genomics/tracy/releases), as a singularity container [SIF file](https://github.com/gear-genomics/tracy/releases) or as a [Docker container](https://hub.docker.com/r/geargenomics/tracy/).

[Source Code](https://github.com/gear-genomics/tracy/)

[Web Applications](https://www.gear-genomics.com)

[Documentation](https://www.gear-genomics.com/docs/tracy/)

## Citation

Rausch, T., Fritz, M.H., Untergasser, A. and Benes, V.
Tracy: basecalling, alignment, assembly and deconvolution of sanger chromatogram trace files.
BMC Genomics 21, 230 (2020).
[https://doi.org/10.1186/s12864-020-6635-8](https://doi.org/10.1186/s12864-020-6635-8)

## License

Tracy is distributed under the BSD 3-Clause license. Consult the accompanying [LICENSE](https://github.com/gear-genomics/tracy/blob/master/LICENSE) file for more details.