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https://github.com/haradama/plasmidpicker

Software to identify plasmid sequence data from metagenome using logistic regression and Minhash
https://github.com/haradama/plasmidpicker

bioinformatics classification contigs fasta logistic-regression metagenome metagenomics minhash plasmids

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Software to identify plasmid sequence data from metagenome using logistic regression and Minhash

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![PlasmidPicker](./logo.png)

## Requirements
- numpy
- cython
- biopython
- click
- mmh3
- h5py

## Installation

```
git clone https://github.com/haradama/PlasmidPicker.git
cd PlasmidPicker
pip install -r requirements.txt
python setup.py install
```

## Usage

```
Usage: plasmidpicker pick [OPTIONS]

Detect plasmid sequence data in metagenome.

Options:
-i, --infile TEXT Specify FASTA input file
-t, --threshold INTEGER Threshold of probability (0 <= t <= 100) [70]
-l, --length INTEGER Threshold of contig's length [1000]
-d, --outdir TEXT Output directory [.]
--help Show this message and exit.
```

```
Usage: plasmidpicker classify [OPTIONS]

Perform similarity search of plasmids using MinHash.

Options:
-i, --infile TEXT Specify FASTA input file
-s, --sketch INTEGER Sketch size (1 <= s <=1000) [1000]
-l, --length INTEGER Threshold of contig's length [1000]
-hi, --hits INTEGER Number of hits to display [5]
-o, --outfile TEXT Output file [result.txt]
--help Show this message and exit.
```

## License