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https://github.com/jackhump/ftld-tdp_analysis

"All code written for Transcriptomic analysis of frontotemporal lobar degeneration with TDP-43 pathology reveals cellular alterations across multiple brain regions"
https://github.com/jackhump/ftld-tdp_analysis

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"All code written for Transcriptomic analysis of frontotemporal lobar degeneration with TDP-43 pathology reveals cellular alterations across multiple brain regions"

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README

          

## FTLD-TDP differential expression

Rahat Hasan 2021

All Rmarkdown workbooks written for the project.

Most workbooks are presented as raw .Rmd (in `scripts/` and knitted HTML files (in `html/`).

## Differential gene expression analysis
[Covariate selection and PCA](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_pca.html)

[FTLD-TDP vs control results](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_differential_expression.html)

[C9orf72 & non-C9orf72 vs control results](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_differential_expression_c9.html)

[Postmortem interval results](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_differential_expression_pmi.html)

## Cellular profiling of DEGs
[Gene-set enrichment analysis (GSEA)](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_gsea.html)

[Expression-weighted cell-type enrichment (EWCE)](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_ewce.html)

[VEN and MMP gene expression](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_vens_and_mmps.html)

[Building sets of marker genes from Mathys et al](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/scripts/FTLD_deconvolution_pipeline.rmd)

[Cell-type deconvolution](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_deconvolution_results.html)

## Differential transcript usage analysis
[DTU results & overlaps with TDP-43 knockdown genes](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_differential_transcript_usage.html)

[Enrichment results using DTU genes](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_differential_transcript_usage_gsea.html)

## Correlations with deconvolution estimates
[All atrophy correlations](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_atrophy_correlations.html)

[Microglial burden correlations](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_microglia_correlations.html)

[DNA methylation correlations](https://github.com/jackhump/FTLD-TDP_analysis/blob/main/html/FTLD_dna_methylation.html)