https://github.com/jayemscript/biosequence-analyzer-api
FastAPI-based REST API for DNA/RNA sequence analysis powered by Biopython. Supports sequence transformation, GC content, and FASTA parsing.
https://github.com/jayemscript/biosequence-analyzer-api
bioinformatics biopython computational-biology dna-analysis fasta fastapi genomics rest-api rna-seq-analysis scientific-computing
Last synced: about 20 hours ago
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FastAPI-based REST API for DNA/RNA sequence analysis powered by Biopython. Supports sequence transformation, GC content, and FASTA parsing.
- Host: GitHub
- URL: https://github.com/jayemscript/biosequence-analyzer-api
- Owner: jayemscript
- License: mit
- Created: 2026-04-22T03:11:00.000Z (about 2 months ago)
- Default Branch: main
- Last Pushed: 2026-04-22T04:12:20.000Z (about 2 months ago)
- Last Synced: 2026-04-22T05:19:23.127Z (about 2 months ago)
- Topics: bioinformatics, biopython, computational-biology, dna-analysis, fasta, fastapi, genomics, rest-api, rna-seq-analysis, scientific-computing
- Language: Python
- Homepage:
- Size: 10.7 KB
- Stars: 0
- Watchers: 0
- Forks: 0
- Open Issues: 0
-
Metadata Files:
- Readme: README.md
- Contributing: .github/CONTRIBUTING.md
- License: LICENSE.md
- Security: SECURITY.md
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README
# biosequence-analyzer-api
## Overview
biosequence-analyzer-api is a REST API built with FastAPI and Biopython for DNA and RNA sequence analysis. It provides core bioinformatics utilities such as sequence transformation, GC content calculation, reverse complement generation, transcription, translation, and FASTA parsing.
The project is designed as a backend-only service intended for integration into other applications, pipelines, or bioinformatics tools.
## Features
* DNA/RNA sequence analysis
* GC content calculation
* Reverse complement generation
* DNA transcription (DNA → RNA)
* RNA/DNA translation (RNA → protein)
* FASTA file parsing support
* RESTful API design for easy integration
## Tech Stack
* Python
* FastAPI
* Biopython
* Uvicorn (ASGI server)
## Project Structure
app/
main.py # Application entry point
routes/ # API route definitions
services/ # Core sequence processing logic
schemas/ # Request and response models
## Installation
### 1. Clone the repository
```
git clone https://github.com/your-username/biosequence-analyzer-api.git
cd biosequence-analyzer-api
```
### 2. Create virtual environment
```
python -m venv venv
source venv/bin/activate # Linux/Mac
venv\Scripts\activate # Windows
```
### 3. Install dependencies
```
pip install -r requirements.txt
```
## Running the API
Start the development server:
```
uvicorn app.main:app --reload
```
The API will be available at:
```
http://127.0.0.1:8000
```
Interactive API documentation:
```
http://127.0.0.1:8000/docs
```
## API Endpoints
### Analyze Sequence
**POST** `/analyze`
Request body:
```
{
"sequence": "ATGCATGC"
}
```
Response:
```
{
"gc_content": 50.0,
"reverse_complement": "GCATGCAT",
"rna": "AUGCAUGC",
"protein": "MHA"
}
```
## Planned Improvements
* FASTA file upload support
* Batch sequence processing
* Authentication layer (optional)
* Rate limiting
* Docker support
* Cloud deployment configuration
## Use Cases
* Bioinformatics research tools
* Educational platforms for molecular biology
* Backend service for genomics applications
* Integration into scientific data pipelines
## License
MIT License
## Notes
This project focuses on backend processing and does not include a frontend. It is intended to be consumed as a standalone API service.