https://github.com/jayrbolton/wigglesworthia-glossinidia
Knowledge compendium on the bacterium _Wigglesworthia glossinidia_
https://github.com/jayrbolton/wigglesworthia-glossinidia
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Knowledge compendium on the bacterium _Wigglesworthia glossinidia_
- Host: GitHub
- URL: https://github.com/jayrbolton/wigglesworthia-glossinidia
- Owner: jayrbolton
- Created: 2019-09-14T21:44:12.000Z (over 5 years ago)
- Default Branch: master
- Last Pushed: 2019-09-25T23:02:36.000Z (over 5 years ago)
- Last Synced: 2025-01-04T23:41:21.306Z (4 months ago)
- Language: Jupyter Notebook
- Size: 156 KB
- Stars: 0
- Watchers: 1
- Forks: 0
- Open Issues: 0
-
Metadata Files:
- Readme: README.md
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README
# Wigglesworth Glossinidia
Knowledge compendium on the bacterium _Wigglesworth Glossinidia_, an obligate endosymbiont of the Tsetse fly.
Overview: this organism is an obligate endosymbiont for the tsetse fly Glossina brevipalpis, Glossina tachinoides, Glossina palpalis palpalis, and Glossina austeni. The tsetse fly is a vector for African trypanosomes, and is the main transmitter of deadly diseases in animals and humans in Africa. The fly feeds on a restricted diet, exclusively consisting of vertebrate blood, and lacks certain metabolic compounds needed for survival and reproduction. To complement this lack in nutrients, the tsetse fly relies mainly on the intracellular bacterial symbiont, Wigglesworthia glossinidia for its viability and fecundity.
_General attributes_
* [Gram-negative](https://en.wikipedia.org/wiki/Gram-negative_bacteria)
* [Rod-shaped](https://microbewiki.kenyon.edu/index.php/File:Wiggles3.gif)
_Taxonomy_
[NCBI Taxonomy:](https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=51229)
* Bacteria
* Proteobacteria
* Gammaproteobacteria
* Enterobacterales
* Erwiniaceae
* Wigglesworthia
* Wigglesworthia glossinidia
[GTDB Taxonomy:](https://gtdb.ecogenomic.org)
* Bacteria
* Proteobacteria
* Gammaproteobacteria
* Enterobacterales
* Enterobacteriaceae
* Wigglesworthia
* Wigglesworthia glossinidia
## Genbank sequences
Source:
### Endosymbiont of Glossina brevipalpis
_Genome attributes_
* Strain _W. brevipalpis_: https://www.ncbi.nlm.nih.gov/pubmed/12219091
* 697,742 kbp
* GC ratio: .22
* Protein and RNA coding regions: 89%
* CDSs - 621
* tRNAs - 34
* Genes unique to W. glossinidia - 3
* Chromosome source file: [./sequence_data/NC_004344/NC_004344.2.gb](./sequence_data/NC_004344/NC_004344.2.gb)
* GC content: 0.22478946 (TODO provenance)
* Skew diagram TODO
* Plasmid (pWb1) source file: [./sequence_data/NC_003425/NC_003425.1.gb](./sequence_data/NC_003425/NC_003425.1.gb)
* GC content: 0.21969697 (TODO provenance)
### Endosymbiont of Glossina morsitans morsitans (Yale colony), complete genome.
* Chromosome source file: [./sequence_data/NC_016893/NC_016893.1.gb](./sequence_data/NC_016893/NC_016893.1.gb)
* GC content: 0.25223652 (provenance TODO)
* Skew diagram TODO
## Endosymbiont variations
This bacterium can be an endosymbiont of:
* Glossina austeni
* Glossina brevipalpis
*
*
*
* Glossina fuscipes
* Glossina fuscipes fuscipes
* Organism modifiers:
* Glossina fuscipes martinii
* Organism modifiers:
* Glossina fuscipes quanzensis
* Organism modifiers:
* Glossina longipennis
* Glossina morsitans
* Glossina morsitans morsitans (Yale colony)
*
*
*
* Glossina pallidipes
* Glossina palpalis
* Glossina palpalis gambiensis
* Glossina palpalis palpalis
* Organism modifers:
* Glossina tachinoides
## Origin of replication
Possibly 2 origins of replication: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269012/
## Questions
* How different are sequences of the strains?
* Why the difference between GTDB and NCBI taxa names?
## References
### Publications
* [Insight into the transmission biology and species-specific functional capabilities of tsetse (Diptera: glossinidae) obligate symbiont Wigglesworthia.](https://www.ncbi.nlm.nih.gov/pubmed/22334516)
* Cryptic Diversity within the Major Trypanosomiasis Vector Glossina fuscipes Revealed by Molecular Markers
* [Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia.](https://www.ncbi.nlm.nih.gov/pubmed/12219091)
* Influence of host phylogeographic patterns and incomplete lineage sorting on within-species genetic variability in wigglesworthia species, obligate symbionts of tsetse flies
_Interesting attributes_
* Vital for the immune system of the Tsetse fly
* Heavily involved in synthesis of vitamins for its host
* Has eliminated genes in the host's fly's genome
* Very small genome at only ~700kbp
* Possibly two origins of replication
* [GC and AT skew diagrams](./gc-skew/skew.ipynb)
* https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269012/
* They have genese for motility, but are not actually motile
* Its A/T counts are each almost 3.5x that of the G/C counts
_Other notes_
* Cannot be cultivated in a laboratory [[1]](https://microbewiki.kenyon.edu/index.php/Wigglesworthia)
## Resources
* Bacmap genome atlas: http://wishart.biology.ualberta.ca/BacMap/cgi/getSpeciesCard.cgi?accession=NC_004344
* Microbe Wiki: https://microbewiki.kenyon.edu/index.php/Wigglesworthia
* BioCyc: https://biocyc.org/organism-summary?object=GCF_000008885