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https://github.com/jfjlaros/demultiplex

Versatile FASTA/FASTQ demultiplexer.
https://github.com/jfjlaros/demultiplex

demultiplex fasta fastq ngs

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Versatile FASTA/FASTQ demultiplexer.

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Demultiplex: FASTA/FASTQ demultiplexer
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Versatile NGS demultiplexer with the following features:

- Support for FASTA and FASTQ files.
- Support for gzip and bzip2 compressed files.
- Support for multiple reads per fragment, e.g., paired-end.
- Handles barcodes in the header and in the reads.
- Handles barcodes at *unknown* locations in reads (e.g., PacBio or Nanopore
barcodes).
- Support for selection of part of a barcode.
- Allows for mismatches, insertions and deletions.
- Barcode guessing by frequency or fixed amount.
- Handles large numbers (over one million) of barcodes.

Please see ReadTheDocs_ for the latest documentation.

.. _ReadTheDocs: https://demultiplex.readthedocs.io/en/latest/index.html