Ecosyste.ms: Awesome

An open API service indexing awesome lists of open source software.

Awesome Lists | Featured Topics | Projects

https://github.com/jumitti/transfectionratioxpert

Just a Streamlit tool to calculate ratio of DNA for transfection
https://github.com/jumitti/transfectionratioxpert

bioinfor biology python science streamlit streamlit-application streamlit-webapp transfection

Last synced: 3 days ago
JSON representation

Just a Streamlit tool to calculate ratio of DNA for transfection

Awesome Lists containing this project

README 

        
# 🧬 Transfection Ratio Xpert



### ⚠ THE PROJECT HAS BEEN COMPILED IN [LABMASTER](https://github.com/Jumitti/labmaster)



**🌐 Application is accessible [HERE](https://labmaster.streamlit.app/)**



**🖥️ Standalone application for WINDOWS is accessible [HERE](https://github.com/Jumitti/TransfectionRatioXpert/releases)**



**📄 Full documentation [HERE](https://jumitti.notion.site/Transfection-Ratio-Xpert-c25f10537e454529a4397925fe9e68c5?pvs=74)**



Cellular transfection is a fundamental technique in molecular biology for introducing nucleic acids, such as DNA or RNA, 

into living cells. This method plays a crucial role in many areas of research, including cell biology, biotechnology and 

gene therapy. By enabling targeted genetic manipulation and the study of cellular mechanisms, cell transfection offers 

promising opportunities for the understanding of biological processes and the development of new therapies.



Transfection efficiency often depends on several factors, including the type of cells used, the transfection method, 

and optimal ratios of nucleic acids to transfection agents. Among the most commonly used transfection agents are 

Lipofectamine 2000, Lipofectamine 3000 from Invitrogen, and jetPRIME from Polyplus-transfection. These reagents are 

designed to enhance the uptake and delivery of nucleic acids into cells, thereby facilitating the transfection process.



## About Transfection Ratio Xpert

This application aims to simplify the process of calculating the necessary ratios between DNA and transfection agents 

during cell transfection. By providing a user-friendly interface and advanced calculation tools, it allows researchers 

and scientists to optimize their transfection experiments, explore different parameters and generate personalized 

experimental conditions. Please note that this application is intended for calculation assistance purposes only and 

may contain errors. We recommend that you independently verify the results obtained before making decisions based on them.



## Transfection Ratio Xpert Features

**Full documentation, protocols and troubleshooting [HERE](https://jumitti.notion.site/Transfection-Ratio-Xpert-c25f10537e454529a4397925fe9e68c5?pvs=74)**

- Instant preparation of multiple transfection conditions, without number restrictions.

- Support for co-transfection with multiple DNAs, without limits.

- Total flexibility to adjust the amount of DNA to transfect.

- Selection of the transfectant among Lipofectamine and jetPRIME, with ratio DNA/transfectant.

- Adaptation to a wide range of plate formats, from 96-well to 150 mm culture vessel.

- Efficient calculation of the number of wells required based on the specified transfection parameters.



### Credit

Created by [Minniti Julien](https://github.com/Jumitti) under [MIT licence](https://github.com/Jumitti/TransfectionRatioXpert/blob/main/LICENSE)