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https://github.com/keller-mark/vitessce-demo-sc-atac-seq-10x-genomics

Data processing for a demo of vitessce + higlass
https://github.com/keller-mark/vitessce-demo-sc-atac-seq-10x-genomics

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Data processing for a demo of vitessce + higlass

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README

        

Vitessce branch: [`keller-mark/sc-atac-demo-v2`](https://github.com/hubmapconsortium/vitessce/tree/keller-mark/sc-atac-demo-v2)

Demo: http://s3.amazonaws.com/vitessce-data/demos/2020-06-22/24f33f0/index.html?dataset=sc-atac-10x-pbmc&theme=light

Set up and activate conda environment:

```sh
conda env create -f environment.yml
conda activate vitessce-demo-sc-atac-seq-10x-genomics
```

Run snakemake:

```sh
snakemake --cores 1
```

Ingest the tilesets for all 10 clusters, using [higlass-server](https://github.com/higlass/higlass-server).
The script takes as an argument the relative path to the higlass server directory.

```sh
bash run_ingest.sh ../../higlass/higlass-server
```

Note: the above command may generate the following warnings `tilesets.models.DoesNotExist: Tileset matching query does not exist.`