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https://github.com/luispedro/coelho2013_bioinformatics

Reproducible code archive for http://dx.doi.org/10.1093/bioinformatics/btt392
https://github.com/luispedro/coelho2013_bioinformatics

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Reproducible code archive for http://dx.doi.org/10.1093/bioinformatics/btt392

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README

          

Source code for paper *Determining the subcellular location of new proteins
from microscope images using local features* by Coelho et al. in
`Bioinformatics `__

This repository is **for reproduction of the results in the paper**. If you
want to apply the methods to your data, check out the `tutorial on doing so
`__.
It is a step by step manual on applying the methods to your data.

Dependencies
------------

::

sudo apt-get install python python-pip python-virtualenv
sudo apt-get install dvipng

Instructions
------------

1. For the Human Protein Atas data, please download from
http://murphylab.web.cmu.edu/software/2012_PLoS_ONE_Reannotation/

Edit the file ``sources/hpa.py`` to point to where you downloaded all the data.

2. Get the randtag data from
http://murphylab.web.cmu.edu/software/2013_Bioinformatics_LocalFeatures/ or
from `Data Dryad `__

3. The remaining data should be automatically downloaded when you run::

doitall.sh

This will also run the computation.

If you want to take advantage of multiple processors, edit the file
``doitall.sh`` and set the ``NR_CPUS`` variable. Note that the whole
computation (i) takes a very long time (days) on a single core and (ii) is
designed to take full advantage of multiples cores.

Citation
--------

For referring to this work, please cite:

*Determining the subcellular location of new proteins from microscope images
using local features* by Luis Pedro Coelho, Joshua D. Kangas, Armaghan Naik,
Elvira Osuna-Highley, Estelle Glory-Afshar, Margaret Fuhrman, Ramanuja
Simha, Peter B. Berget, Jonathan W. Jarvik, and Robert F. Murphy (2013).
Bioinformatics, [`DOI `__]