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https://github.com/mmasoud1/histojs

HistoJS: Web-Based Analytical Tool for Multiplexed Images. Limited Github Online Demo 👇
https://github.com/mmasoud1/histojs

cell-cell-interaction cell-segmentation digital-slide-archive image-processing mibiscope multichannel multiplexed-imaging multiplexed-imaging-analysis ome-tiff phenotyping proteomics proteomics-data-analysis single-cell spatial-biology spatial-data-analysis whole-slide-imaging

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HistoJS: Web-Based Analytical Tool for Multiplexed Images. Limited Github Online Demo 👇

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## HistoJS [![Version](https://img.shields.io/badge/Version-1.0.0-brightgreen)]() [![DOI](https://joss.theoj.org/papers/10.21105/joss.06317/status.svg)](https://joss.theoj.org/papers/10.21105/joss.06317)

[![HistoJS logo](https://lh3.googleusercontent.com/Q3eYJfpQMety3sUUTLKeZKLkJBzx-NmLZNn1NphdWs9hBNYmfHCzZTphddpXDcfUxjtaHTXTr89R57LieZflqUsGHM3TxtIbVNoQOKf3ZbPjB01C8gYANmezAzq4JgUU=w1823)]()

[![MIT-License ](https://img.shields.io/badge/license-MIT-green)](https://github.com/Mmasoud1/HistoJS/blob/main/LICENSE) [![Python ](https://img.shields.io/badge/python-3.5%20%7C%203.6%20%7C%203.7-blue)]()[![JS ](https://img.shields.io/badge/Types-JavaScript-blue)]()
[![HW ](https://img.shields.io/badge/HardWare-GPU-green)]()

**[Demo](https://mmasoud1.github.io/HistoJS)   [Updates](#Updates)   [Doc](https://github.com/mmasoud1/HistoJS/wiki/)   [News!](#News)**




HistoJS is a new web-based interactive tool designed to overcome the challenges of using highly-multiplexed immunofluorescence (HMIF) images for spatial biology research. HistoJS provides open-source and extensible tools for analyzing spatial-molecular patterns, offering a deeper understanding of single-cell spatial relationships. It also features machine learning algorithms in a user-friendly interactive interface for the biomedical community.


The tool can manage, store, and analyze the multi-channel OME-Tiff files using Digital Slide Archive as a backbone to update the image metadata remotely or locally.

## Live Demo

HistoJS [Demo](https://mmasoud1.github.io/HistoJS) hosted by Github pages shows most core functionalities.

The analysis mode needs running of the Flask RestAPIs as the steps described [here](https://github.com/Mmasoud1/HistoJS/wiki/Setup-Environment).

## News!

* HistoJS abstract is accepted for poster presentation in the SIAM 2024 Conference on Imaging Science ([IS24](https://www.siam.org/conferences/cm/conference/is24)) in Atlanta, GA.




* HistoJS [paper](https://joss.theoj.org/papers/10.21105/joss.06317) is published in the Journal of Open Source Software (JOSS) on Feb 07, 2024.




* HistoJS abstract is accepted for poster presentation in the [OHBM](https://www.humanbrainmapping.org/) 2024 Annual Meeting in Seoul, Korea.




## Citation

HistoJS [paper](https://joss.theoj.org/papers/10.21105/joss.06317) for v1.1.0 is published on Feb 07, 2024, in the Journal of Open Source Software (JOSS) [![DOI](https://joss.theoj.org/papers/10.21105/joss.06317/status.svg)](https://joss.theoj.org/papers/10.21105/joss.06317)


For **APA** style, the paper can be **cited** as:

> Masoud, M., Gutman, D., & Plis, S. (2024). HistoJS: Web-Based Analytical Tool for Advancing Multiplexed Images. Journal of Open Source Software, 9(94), 6317. https://doi.org/10.21105/joss.06317


For **BibTeX** format that is used by some publishers, please use:

```BibTeX:
@article{Masoud_HistoJS_Web-Based_Analytical_2024,
author = {Masoud, Mohamed and Gutman, David and Plis, Sergey},
doi = {10.21105/joss.06317},
journal = {Journal of Open Source Software},
month = feb,
number = {94},
pages = {6317},
title = {{HistoJS: Web-Based Analytical Tool for Advancing Multiplexed Images}},
url = {https://joss.theoj.org/papers/10.21105/joss.06317},
volume = {9},
year = {2024}
}
```


## Updates

https://github.com/Mmasoud1/HistoJS/assets/36082112/7598afb0-ef05-40e0-9d53-767fbc0e6d99

**HistoJS Design Mode (Data source: [Rashid et al](https://doi.org/10.7303/syn17865732))**


https://github.com/Mmasoud1/HistoJS/assets/36082112/7ba5c733-3b31-47e9-9cc4-e9fb606d42da

**HistoJS V1.0.0 Analysis Mode**


[![Google Doc](https://img.shields.io/badge/HistoJS-Feedback-blue)](https://docs.google.com/forms/d/e/1FAIpQLSdHuO--mG00sKydQpJ7sPpDmhcJ4ECdj-wAB1kwXQExh_nUSg/viewform?usp=sf_link) [![MIT-License ](https://img.shields.io/badge/license-MIT-green)](https://github.com/Mmasoud1/HistoJS/blob/main/LICENSE)

**Mohamed Masoud - 2023**