https://github.com/schultzm/p2xkit

Primer-to-x kit, where x is 'isPCR', 'qPCR'
https://github.com/schultzm/p2xkit

Last synced: 9 days ago
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Primer-to-x kit, where x is 'isPCR', 'qPCR'

• Host: GitHub
• URL: https://github.com/schultzm/p2xkit
• Owner: schultzm
• License: agpl-3.0
• Created: 2020-04-01T03:05:19.000Z (about 6 years ago)
• Default Branch: master
• Last Pushed: 2021-02-10T10:14:23.000Z (over 5 years ago)
• Last Synced: 2026-06-14T20:33:35.725Z (9 days ago)
• Language: Python
• Homepage:
• Size: 14.3 MB
• Stars: 0
• Watchers: 0
• Forks: 0
• Open Issues: 1
• Metadata Files:
  • Readme: README.md
  • License: LICENSE

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README

          
# p2xkit

[![CircleCI](https://circleci.com/gh/schultzm/p2xkit.svg?style=svg&circle-token=6be92d4e84abcddc32721c2c507b07c08810e327)](https://app.circleci.com/pipelines/github/schultzm/p2xkit)

`ispcr` Uses `biopython`, `emboss:primersearch` to align primers to template to find amplimers (in-silico PCR).  

`qpcr` does same as `ispcr` but adds qPCR probes to the mix (in-silico qPCR).

To use:  

```{bash}

p2xkit

usage: p2xkit [-h]  ...

optional arguments:

  -h, --help  show this help message and exit

Sub-commands help:

  

    ispcr     Perform in-silico PCR.

    qpcr      Perform in-silico qPCR.

    test      Run p2xkit unittests

    version   Print version to stdout

```

Under development