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https://github.com/slowkow/cellguide

🧭 Navigate single-cell RNA-seq datasets in your web browser.
https://github.com/slowkow/cellguide

bioinformatics cellbrowser data-visualization javascript scrna-seq

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🧭 Navigate single-cell RNA-seq datasets in your web browser.

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# Cell Guide 🧭

[![DOI](https://zenodo.org/badge/268866845.svg)](https://zenodo.org/badge/latestdoi/268866845)

Navigate single-cell RNA-seq datasets in your web browser.

Try it at https://cell.guide

### Features

- Display metadata on the UMAP figure
- Display gene expression on the UMAP figure
- Aggregate statistics for cell clusters and metadata categories
- Find any gene, instantly
- Browse statistics for every gene


Flip through metadata variables and see them in low-dimensional space.











See which donors have similar proportions of different cell clusters.


Find any gene and see expression across clusters, faceted by metadata variables.





Demo scRNA-seq data from the research article by [Smillie et al. 2019][3].

[3]: https://doi.org/10.1016/j.cell.2019.06.029

## Quick Start

### Step 1. Install Cell Browser

We can install the Cell Browser Python package with pip (we need a specific version):

```
pip install cellbrowser==0.7.15
```

Or see the [Cell Browser installation instructions](https://cellbrowser.readthedocs.io/installation.html).

### Step 2. Create a browser for your data

See the [documentation][1] for how to create a Cell Browser for your data.

When your browser is working, it should look something like this:



### Step 3. Copy Cell Guide files

```bash
# Get the Cell Guide code
curl -s https://codeload.github.com/slowkow/cellguide/tar.gz/master | tar xvz

# Overwrite the Cell Browser files: index.html js/ css/ ext/ img/
command cp -rf cellguide-master/www/* public_html/
```

For a complete example, see the [demo.sh](demo.sh) script.

Make sure to refresh your web browser with ⌘+Shift+R (or Ctrl+Shift+R on Windows).

[1]: https://cellbrowser.readthedocs.io/basic_usage.html

## Dependencies

The current version of Cell Guide depends on [Cell Browser] by [Maximilian
Haeussler].

[Cell Browser]: https://github.com/maximilianh/cellBrowser
[Maximilian Haeussler]: https://github.com/maximilianh

Built with:

- [Bootstrap](https://getbootstrap.com)
- [CSS loaders](https://projects.lukehaas.me/css-loaders/)
- [d3-legend](https://github.com/susielu/d3-legend)
- [D3.js](https://d3js.org)
- [FastBitSet](https://github.com/lemire/FastBitSet.js/)
- [hcluster.js](https://github.com/cmpolis/hcluster.js/)
- [jQuery](https://jquery.com)
- [JSURL2](https://www.npmjs.com/package/@yaska-eu/jsurl2)
- [pako](https://github.com/nodeca/pako)
- [palette.js](https://github.com/google/palette.js/tree/master)
- [papaparse](https://www.papaparse.com/)
- [tablesort](https://github.com/tristen/tablesort)

Let me know what you think! [@slowkow](https://twitter.com/slowkow)