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https://github.com/smithsonian/fabaceae_phylogenomics_workflow

Target-enrichment data processing in legumes
https://github.com/smithsonian/fabaceae_phylogenomics_workflow

legumes molecular-evolution phylogenetics target-enrichment

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Target-enrichment data processing in legumes

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README

          

# Fabaceae phylogenomics workflow
This repository contains instructions on handling target-enrichment (bait-capture) data using [HybPiper](https://github.com/mossmatters/HybPiper) and performing subsequent phylogenetic analyses on the Smithsonian Institution HPC (Hydra cluster). If you don't have your own dataset, you can use tutorial data from [here](https://github.com/mossmatters/HybPiper/tree/master/test_dataset). If you don't have access to the cluster, install all [dependencies](https://github.com/mossmatters/HybPiper#dependencies) and programs used here such as MAFFT, TrimAl, RAxML on your system and run the main commands from the job files. Follow steps in the [Data_processing.md](https://github.com/Smithsonian/Fabaceae_Phylogenomics_workflow/blob/master/Data_processing.md).

Paper:

Vatanparast, M., A. Powell, J. J. Doyle, and A. N. Egan. 2018. Targeting legume loci: A comparison of three methods for target enrichment bait design in Leguminosae phylogenomics. [Applications in Plant Sciences 6(3): e01036](https://onlinelibrary.wiley.com/doi/full/10.1002/aps3.1036)

Datasets are available from Figshare: https://doi.org/10.6084/m9.figshare.c.4040372.v4

Example phylogenetic trees using different species tree reconstruction methods.

![example-tree](https://user-images.githubusercontent.com/13125143/35277516-f3342a4e-003e-11e8-8fa7-9bb5c513a2b0.jpg)