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https://github.com/ssi-dk/ssi_analysis_utility

Package for the species specific analysis WP6 MicrobeSeq
https://github.com/ssi-dk/ssi_analysis_utility

Last synced: 7 months ago
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Package for the species specific analysis WP6 MicrobeSeq

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README

          

# Bioinformatics Snakemake Pipeline

## Overview

This Snakemake pipeline automates microbial genomic analyses,
integrating tools for resistance gene detection, virulence factor
identification, typing, and lineage determination.

## Installation

1. Clone the repository:

``` bash
git clone
cd
```

2. Install dependencies: \`\`\`bash conda env create -f
environment.yaml conda activate

## Running the Pipeline

1. Prepare the samplesheet.tsv:

- Include sample IDs, file paths for reads, and assembly files.
Customize config.yaml:

2. Define tools, databases, and analyses for your samples.

3. Execute Snakemake:
`bash cd workflow snakemake --configfile ../config/config.yaml --cores `

4. Results:

- Output files will be stored in the directory specified by `out_folder`

## Output Structure

Results are organized by species and sample:

results/
├── Species_1/
│ ├── sample1/
│ │ ├── analysis_1/
│ │ ├── analysis_2/
│ │ └── analysis_3/
│ └── sample2/
| ├── analysis_1/
| ├── analysis_2/
| ...
├── Species_1/
│ ├── sample1/
| ├── analysis_1/
| ...

└── logs/