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https://github.com/stuntspt/4pipe4_to_genotyping_array

A script to convert 4Pipe4 SNP output to a genotyping array in FASTA format
https://github.com/stuntspt/4pipe4_to_genotyping_array

Last synced: 12 months ago
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A script to convert 4Pipe4 SNP output to a genotyping array in FASTA format

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#4Pipe4_to_sequenom

###This program will transform a sorted and indexed bam file, plus a FASTA file generated by 4Pipe4 into SNPs and their flanking regions ready for a sequenom Mass Array (c).

###Usage:

python2 4Pipe4_to_sequenom.py infile.bam infile.fasta variation_treshold(float: 0-1) max_variations(int)

The "infile.bam.bai" has to be in the same directory as the "infile.bam".

###Requirements:

* Python 2.x (tested with 2.7)
* pysam (code.google.com/p/pysam/‎)

###License:
GPLv3.