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https://github.com/uclouvain-cbio/sager

Analyse sage results in R
https://github.com/uclouvain-cbio/sager

bioconductor mass-spectrometry proteomics

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Analyse sage results in R

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## `sager`: Analysing sage results with R

The `sager` package can be used to import results produced by the
[sage](https://lazear.github.io/sage/) search engine into R. Sage
produces tab-separated output files for identification and
quantitation results. In `sager`, we import these result files into
establised Bioconductor classes:

- Identification results are parsed and imported as
[PSM](https://rformassspectrometry.github.io/PSMatch/articles/PSM.html)
objects with
[sagePSM()](https://uclouvain-cbio.github.io/sager/reference/sagePSM.html).

- Quantitation (and identification) results are parsed, merged and
imported as
[QFeatures](https://rformassspectrometry.github.io/QFeatures/articles/QFeatures.html)
objects with
[sageQFeatures()](https://uclouvain-cbio.github.io/sager/reference/sageQFeatures.html).

These functions are described and demonstrated in their respective
manual pages using data generated from Yu *et al.* [Benchmarking the
Orbitrap Tribrid Eclipse for Next Generation Multiplexed
Proteomics](https://doi.org/10.1021/acs.analchem.9b05685), downloaded
from ProteomeXchange project
[PXD016766](https://www.ebi.ac.uk/pride/archive/projects/PXD016766). See
[sagerData()](https://uclouvain-cbio.github.io/sager/reference/sagerData.html)
for details.

If you want to install and try the package out:

```
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")

BiocManager::install("UCLouvain-CBIO/sager")
```

If you run into issues of have questions, please open [an
issue](https://github.com/UCLouvain-CBIO/sager/issues).