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https://github.com/jackhump/fus_intron_retention

Scripts written for the FUS NLS mutation comparison with FUS KO project
https://github.com/jackhump/fus_intron_retention

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Scripts written for the FUS NLS mutation comparison with FUS KO project

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# *FUS* ALS-causative mutations impact FUS autoregulation and the processing of RNA-binding proteins through intron retention

**Jack Humphrey**

[paper now published at Nucleic Acids Research](https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa410/5849905)

All code written in R and presented as Rmarkdown documents for readability.

Accompanying scripts:

#### 01_differential_expression/
- 01_DESeq_joint_modelling.Rmd
- 02_iCLIP_gene_expression.Rmd
#### 02_differential_splicing/
- 01_SGSeq_joint_modelling.Rmd
- 02_SGSeq_variant_function.Rmd
- 03_SGSeq_plotting.Rmd
- 04_compare_expression_splicing.Rmd
#### 03_autoregulation/
- 01_FUS_intron_retention_plotting.Rmd
- 02_Autoregulation_validation.Rmd
#### 04_comparing_other_data/
- 01_TDP_cross_regulation.Rmd
- 02_Luisier_VCP_cross_regulation.Rmd
#### 05_heterozygotes/
- Comparing_Hets.Rmd
#### 06_CLIPdb_FUS_introns/
- retained_intron_clip.R