https://github.com/quantixed/p062p035
Data and code for manuscript p062p035 - Shelford, Burgess et al., 2025
https://github.com/quantixed/p062p035
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Data and code for manuscript p062p035 - Shelford, Burgess et al., 2025
- Host: GitHub
- URL: https://github.com/quantixed/p062p035
- Owner: quantixed
- License: gpl-3.0
- Created: 2023-03-15T10:32:54.000Z (almost 3 years ago)
- Default Branch: main
- Last Pushed: 2025-02-20T09:17:36.000Z (12 months ago)
- Last Synced: 2025-07-09T09:51:05.470Z (7 months ago)
- Topics: paper
- Language: HTML
- Homepage:
- Size: 4.44 MB
- Stars: 1
- Watchers: 1
- Forks: 0
- Open Issues: 0
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Metadata Files:
- Readme: README.md
- License: LICENSE
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README
# p062p035
Data and code for manuscript `p062p035`
**Structural characterization and inhibition of the interaction between ch-TOG and TACC3**
James Shelford\*, Selena G. Burgess\*, Elena Rostkova, Mark W. Richards, Gabrielle Larocque, Josephina Sampson, Christian Tiede, Alistair J. Fielding, Tina Daviter, Darren C. Tomlinson, Antonio N. Calabrese, Mark Pfuhl\*\*, Richard Bayliss\*\* and Stephen J. Royle\*\*
*J Cell Biol* (2025) 224: e202407002, doi: [10.1083/jcb.202407002](https://doi.org/10.1083/jcb.202407002).
## Code and data for recreation of plots
### Main Figures
- mCherry-Affimer IP quantification - `IP_Quantification`
- Spindle ch-TOG quantification - `Spindle_Quantification`
- Spindle tubulin quantification - `gl_tub`
- Pericentrin quantification - `Pericentrin_quant`
- Centrin-pericentrin quantification - `Centrin-pericentrin`
- ch-TOG mutant pericentrin - `chTOG_mut_pcnt`
- Sankey diagram - `Sankey`
- Mitotic timing - `MitoticTiming`
### Supplementary Figures
- 3D Mitotic spindle analytics - `SpindleAnalytics`
- Affimer knocksideways - `JS155`
- Clathrin/TACC3 quantification - `gl_clat`
- PCNT foci with nocodazole - `gl_noc`
### Notes
The R/Igor code can be executed with data placed into the `Data` folder of the RProject.
Fiji scripts for image analysis are included in the `Script` folder of the respective project.
Raw data (images) are not included for space reasons, only text outputs from image analysis steps.
## Development
Some code in this repo was developed elsewhere or is still under development.
The version in this repo reflects the state at the time the figures were assembled.
[`https://github.com/james-shelford/Image_Analysis_Projects`](https://github.com/james-shelford/Image_Analysis_Projects)
[`https://github.com/james-shelford/SpindleAnalytics`](https://github.com/james-shelford/SpindleAnalytics)
[`https://github.com/quantixed/MitoticTiming`](https://github.com/quantixed/MitoticTiming)
## Contributions
R Code and ImageJ Scripts written by James Shelford.
Code review and small changes for publication by Stephen Royle.
Igor code by Stephen Royle.