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https://github.com/timtreis/202504_workshop_gscn


https://github.com/timtreis/202504_workshop_gscn

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scverse workshop in collaboration with the GSCN — GSCN Workshop 2025




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scverse workshop in collaboration with the GSCN










scverse workshop in collaboration with the GSCN#


Welcome to the GSCN Workshop materials! This book contains the workshop materials organized by day:



Each day contains its own set of notebooks and materials for the workshop.

Before the event#

Tech setup#


In addition to the actual workshop, we offer two pre-workshop sessions designed to help participants with any issues installing software required for the workshop. During these, we also encourage participants to ask questions about basic Python/Jupyter Notebook usages (the notebook discussed in these sessions can be found here). The two sessions take place on:



  • Wednesday (02.04.2025), 15:00 - 17:00 CET


  • Thursday (03.04.2025), 15:30 - 17:30 CET



The Zoom links to these events can be found in the mail sent on Sunday, 30.03.2025. If you have any other questions, please direct them to the 2025-04: Workshop GSCN > pre-workshop support Zulip channel.

Syllabus#


Note: Schedule subject to minor adjustments.


Date


Time


Activity

Monday, 07.04.2025


09:00 – 09:15


Welcome & Introduction to the Workshop


09:15 – 10:00


Talk: “An in-depth look at AnnData” by Ilan Gold


10:00 – 11:30


1) Basic pre-processing of scRNA-seq data



2) Batch effect correction



3) Downstream analysis


11:30 - 12:00


Hands-on coding & Troubleshooting


12:00 – 13:00


Lunch


13:00 – 13:30


Intro to “Trajectory analysis”


13:30 – 13:40


Break


13:40 – 15:20


Trajectory analysis


15:20 – 15:40


Break


15:40 – 17:30


Trajectory analysis


19:00 - tbd


Dinner at Porta Nova (Robert-Koch-Platz 12)



Tuesday, 08.04.2025


09:00 – 09:30


Recap and Q&A, tech setup for spatial workshop


09:30 – 10:00


Talk: “Clonal dynamics and mitochondrial genetics through the
lens of single-cell multi-omics” by Leif Ludwig


10:00 – 11:30


1) Ligand-receptor interaction analysis



2) Differential cellular abundance


11:30 - 12:00


Hands-on coding & Troubleshooting


12:00 – 13:00


Lunch


13:00 – 13:30


Intro to “Spatial Omics”


13:30 – 13:40


Break


13:40 – 15:20


Spatial preprocessing & analysis


15:20 – 15:40


Break


15:40 – 17:30


Spatial preprocessing & analysis


20:00 - tbd


Dinner (self-organised)



Wednesday, 09.042025


09:00 – 09:30


Talk: “Spatiotemporal dynamics of tumor development in a novel
triple-negative breast cancer model” by Tancredi Massimo Pentimalli


09:30 – 11:30


Talk: “Foxa2 pioneers enteroendocrine lineage recruitment from
intestinal stem cells” by Michael Sterr


10:00 – 11:30


Option A) Reproduce published figures



Option B) Discuss your own data/analysis


11:30 – 12:00


Results / Feedback



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