https://github.com/tuliren/telomere_length_analyzer
Excel VBA script for analysis of telomere length from telomere restriction fragmentation (TRF) Southern blot gel.
https://github.com/tuliren/telomere_length_analyzer
telomere-length telomere-restriction-fragmentation vba-script
Last synced: 8 months ago
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Excel VBA script for analysis of telomere length from telomere restriction fragmentation (TRF) Southern blot gel.
- Host: GitHub
- URL: https://github.com/tuliren/telomere_length_analyzer
- Owner: tuliren
- Created: 2013-11-26T18:25:02.000Z (almost 12 years ago)
- Default Branch: master
- Last Pushed: 2016-10-09T18:54:21.000Z (about 9 years ago)
- Last Synced: 2025-01-31T06:57:24.484Z (10 months ago)
- Topics: telomere-length, telomere-restriction-fragmentation, vba-script
- Homepage: https://github.com/tuliren/telomere_length_analyzer
- Size: 243 KB
- Stars: 0
- Watchers: 3
- Forks: 0
- Open Issues: 0
-
Metadata Files:
- Readme: README.md
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README
Telomere length analyzer
========================
## Application
This Excel script calculates the mean telomere length from a telomeric restriction fragmentation (TRF) Southern blot image. Currently, the maximum number of lanes it can process is **20**.
## Analysis setup
#### Step 0
Evenly partition each lane on the gel image to 30 parts, and measure the color / shade intensity from any gel image software.
#### Step 1
Determine the position of the size ladders / markers in terms of the partition. Set the position of each ladder / marker band in the "Ladders" input region.
#### Step 2
Combine all the intensity data into one column, and copy to the "Volumn" input region.
#### Step 3
Setup the sample information in the next input region.
#### Step 4
Run the script by clicking the button in the fourth section.
## Result
After running the script, three result sheets will be generated.
#### Section II
An overview of the ladder and sample information you have setup in Section I.
#### Section III
The detailed information about the telomere length calculation for each lane.
#### Section IV
A summary of the sample telomere length.
## Copyright
This Excel VBA script program was developed while I was working in laboratory of [Dr. David Gilley](http://genetics.medicine.iu.edu/faculty/david-p-gilley-ph-d/) in Indiana University, School of Medicine, Indianapolis. It was improved based on the algorithm from the script [TeloRun](http://www4.utsouthwestern.edu/cellbio/shay-wright/research/sw_lab_methods.htm) developed in the laboratory of [Dr. Jerry Shay and Dr. Woodring Wrigth](http://www4.utsouthwestern.edu/cellbio/shay-wright/) in University of Texas Southwestern Medical Center. Please consult these two institutions about their policy for intellectual properties.
## Contact
Please email any question or suggestion to [me](tuliren.git@outlook.com).