https://github.com/tuliren/telomere_length_analyzer

Excel VBA script for analysis of telomere length from telomere restriction fragmentation (TRF) Southern blot gel.
https://github.com/tuliren/telomere_length_analyzer

telomere-length telomere-restriction-fragmentation vba-script

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Excel VBA script for analysis of telomere length from telomere restriction fragmentation (TRF) Southern blot gel.

• Host: GitHub
• URL: https://github.com/tuliren/telomere_length_analyzer
• Owner: tuliren
• Created: 2013-11-26T18:25:02.000Z (about 11 years ago)
• Default Branch: master
• Last Pushed: 2016-10-09T18:54:21.000Z (over 8 years ago)
• Last Synced: 2023-10-20T23:33:54.686Z (over 1 year ago)
• Topics: telomere-length, telomere-restriction-fragmentation, vba-script
• Homepage: https://github.com/tuliren/telomere_length_analyzer
• Size: 243 KB
• Stars: 0
• Watchers: 2
• Forks: 0
• Open Issues: 0
• Metadata Files:
  • Readme: README.md

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README

        
Telomere length analyzer

========================

## Application

This Excel script calculates the mean telomere length from a telomeric restriction fragmentation (TRF) Southern blot image. Currently, the maximum number of lanes it can process is **20**.

## Analysis setup

#### Step 0

Evenly partition each lane on the gel image to 30 parts, and measure the color / shade intensity from any gel image software.

#### Step 1

Determine the position of the size ladders / markers in terms of the partition. Set the position of each ladder / marker band in the "Ladders" input region.



#### Step 2

Combine all the intensity data into one column, and copy to the "Volumn" input region.



#### Step 3

Setup the sample information in the next input region.



#### Step 4

Run the script by clicking the button in the fourth section.



## Result

After running the script, three result sheets will be generated.

#### Section II

An overview of the ladder and sample information you have setup in Section I.

#### Section III

The detailed information about the telomere length calculation for each lane.

#### Section IV

A summary of the sample telomere length.



## Copyright

This Excel VBA script program was developed while I was working in laboratory of [Dr. David Gilley](http://genetics.medicine.iu.edu/faculty/david-p-gilley-ph-d/) in Indiana University, School of Medicine, Indianapolis. It was improved based on the algorithm from the script [TeloRun](http://www4.utsouthwestern.edu/cellbio/shay-wright/research/sw_lab_methods.htm) developed in the laboratory of [Dr. Jerry Shay and Dr. Woodring Wrigth](http://www4.utsouthwestern.edu/cellbio/shay-wright/) in University of Texas Southwestern Medical Center. Please consult these two institutions about their policy for intellectual properties.

## Contact

Please email any question or suggestion to [me]([email protected]).